Chromatographic HPV‐16 E6/E7 plasmid vaccine purification employing L‐histidine and 1‐benzyl‐L‐histidine affinity ligands

Affinity chromatography based on amino acids as interacting ligands was already indicated as an alternative compared to ion exchange or hydrophobic interaction for plasmid DNA purification. Understanding the recognition mechanisms occurring between histidine‐based ligands and nucleic acids enables m...

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Veröffentlicht in:	Electrophoresis 2017-11, Vol.38 (22-23), p.2975-2980
Hauptverfasser:	Amorim, Lúcia F. A., Gaspar, Rita, Pereira, Patrícia, Černigoj, Urh, Sousa, Fani, Queiroz, João António, Sousa, Ângela
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Amino acids Aromatic compounds Binding Chromatography Deoxyribonucleic acid DNA Elution Histidine Histidine‐based ligands Hydrophobicity Ions Ligands Monoliths Nucleic acids Phase transitions Plasmid DNA purification Plasmids Purification Ribonucleic acid RNA RNA removal Sample displacement chromatography Selectivity Vaccines
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