Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells
•Mesenchymal stem cells from human tooth germs (hTGSCs) were successfully isolated.•HTGSCs showed a high osteogenic capacity.•STRO-1+ cells were selected to obtain a homogenous subpopulation of hTGSCs.•STRO-1+ subpopulation did not show a superior behavior for mineralization. Mesenchymal stem cells...
Gespeichert in:
| Veröffentlicht in: | Archives of oral biology 2017-10, Vol.82, p.293-301 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 301 |
|---|---|
| container_issue | |
| container_start_page | 293 |
| container_title | Archives of oral biology |
| container_volume | 82 |
| creator | Ercal, Pinar Pekozer, Gorke G. Gumru, Osman Z. Kose, Gamze T. Ramazanoglu, Mustafa |
| description | •Mesenchymal stem cells from human tooth germs (hTGSCs) were successfully isolated.•HTGSCs showed a high osteogenic capacity.•STRO-1+ cells were selected to obtain a homogenous subpopulation of hTGSCs.•STRO-1+ subpopulation did not show a superior behavior for mineralization.
Mesenchymal stem cells derived from the human tooth germ (hTGSCs) are a heterogeneous cell population that can differentiate into osteogenic, neurogenic, and adipogenic lineages. The aim of this study was to compare the osteogenic differentiation capacity of STRO-1 positive (STRO-1+) hTGSCs and unsorted heterogeneous hTGSCs and to establish if STRO-1+ cells are more committed to osteogenic differentiation. HTGSCs were isolated from impacted third molar tooth germ tissues of adolescents, and a subpopulation of STRO-1+ hTGSCs was obtained by fluorescence-activated cell sorting. STRO-1+, STRO-1 negative (STRO-1−), and unsorted cells were cultured in osteogenic and standard culture media to compare their capacity to differentiate towards osteoblastic lineage. Cells were tested for proliferation rates, alkaline phosphatase activity, and amounts of accumulated calcium. Gene expression levels of the RUNX2, osteocalcin, and osteonectin genes were analyzed with real time PCR. Mineralization and osteogenic protein expression were examined by using von Kossa staining and confocal microscopy. Our results indicated that osteogenically induced cell populations showed greater mineralization capacity than non-induced cells. However, expression levels of early and late osteogenic markers were not significantly different between STRO-1+ and unsorted cells. In conclusion, the selection by STRO-1 expression does not yield cells with osteogenic capacity higher than that of the heterogeneous hTGSC population. Cell sorting using osteogenic markers other than STRO-1 might be beneficial in obtaining a more sensitive osteogenic sub-population from unsorted heterogenous hTGSCs. |
| doi_str_mv | 10.1016/j.archoralbio.2017.06.028 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1917362995</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003996917302054</els_id><sourcerecordid>1917362995</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-4ae87eba5e7b164ff93fefe4ad2afa5eb54c2dd6f57324cc685be89d572c8d7a3</originalsourceid><addsrcrecordid>eNqNkFtL5TAURoM46PHyF4b45ktr0ksuj3IYZwRBUOc5pMmOzaFtziSt4L835TjDPAqBTcL69s5eCF1RUlJC2c2u1NH0Ieqh86GsCOUlYSWpxBHaUMFlQVvCjtGGEFIXUjJ5is5S2uVryxg9QaeVYIJJ0WxQfz-5YYHJAA4OP788PRYUJxjAzD5MeD1phvAKkzd4H2aYZq-Hle2XUU94DmHu8SvEEVuI_g0sHiHlfv37mLmcHbGBYUgX6JvTQ4LLz3qOft_9eNn-Kh4ef95vbx8KU3M-F40GwaHTLfCOssY5WTtw0GhbaZdfu7YxlbXMtbyuGmOYaDsQ0ra8MsJyXZ-j60PffQx_FkizGn1af6AnCEtSVFJes0rKNqPygJoYUorg1D76Ucd3RYlaRaud-k-0WkUrwlQWnbPfP8cs3Qj2X_Kv2QxsDwDkZd88RJWMXz1bH7NcZYP_wpgPmj6X3Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1917362995</pqid></control><display><type>article</type><title>Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Ercal, Pinar ; Pekozer, Gorke G. ; Gumru, Osman Z. ; Kose, Gamze T. ; Ramazanoglu, Mustafa</creator><creatorcontrib>Ercal, Pinar ; Pekozer, Gorke G. ; Gumru, Osman Z. ; Kose, Gamze T. ; Ramazanoglu, Mustafa</creatorcontrib><description>•Mesenchymal stem cells from human tooth germs (hTGSCs) were successfully isolated.•HTGSCs showed a high osteogenic capacity.•STRO-1+ cells were selected to obtain a homogenous subpopulation of hTGSCs.•STRO-1+ subpopulation did not show a superior behavior for mineralization.
Mesenchymal stem cells derived from the human tooth germ (hTGSCs) are a heterogeneous cell population that can differentiate into osteogenic, neurogenic, and adipogenic lineages. The aim of this study was to compare the osteogenic differentiation capacity of STRO-1 positive (STRO-1+) hTGSCs and unsorted heterogeneous hTGSCs and to establish if STRO-1+ cells are more committed to osteogenic differentiation. HTGSCs were isolated from impacted third molar tooth germ tissues of adolescents, and a subpopulation of STRO-1+ hTGSCs was obtained by fluorescence-activated cell sorting. STRO-1+, STRO-1 negative (STRO-1−), and unsorted cells were cultured in osteogenic and standard culture media to compare their capacity to differentiate towards osteoblastic lineage. Cells were tested for proliferation rates, alkaline phosphatase activity, and amounts of accumulated calcium. Gene expression levels of the RUNX2, osteocalcin, and osteonectin genes were analyzed with real time PCR. Mineralization and osteogenic protein expression were examined by using von Kossa staining and confocal microscopy. Our results indicated that osteogenically induced cell populations showed greater mineralization capacity than non-induced cells. However, expression levels of early and late osteogenic markers were not significantly different between STRO-1+ and unsorted cells. In conclusion, the selection by STRO-1 expression does not yield cells with osteogenic capacity higher than that of the heterogeneous hTGSC population. Cell sorting using osteogenic markers other than STRO-1 might be beneficial in obtaining a more sensitive osteogenic sub-population from unsorted heterogenous hTGSCs.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2017.06.028</identifier><identifier>PMID: 28686984</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Alkaline Phosphatase - analysis ; Antigens, Surface - physiology ; Calcium - analysis ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dentistry ; Female ; Flow Cytometry ; Humans ; Male ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Microscopy, Confocal ; Molar, Third ; Osteogenesis ; Osteogenesis - physiology ; Real-Time Polymerase Chain Reaction ; STRO-1 ; Tooth germ ; Tooth Germ - cytology ; Young Adult</subject><ispartof>Archives of oral biology, 2017-10, Vol.82, p.293-301</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-4ae87eba5e7b164ff93fefe4ad2afa5eb54c2dd6f57324cc685be89d572c8d7a3</citedby><cites>FETCH-LOGICAL-c377t-4ae87eba5e7b164ff93fefe4ad2afa5eb54c2dd6f57324cc685be89d572c8d7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003996917302054$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28686984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ercal, Pinar</creatorcontrib><creatorcontrib>Pekozer, Gorke G.</creatorcontrib><creatorcontrib>Gumru, Osman Z.</creatorcontrib><creatorcontrib>Kose, Gamze T.</creatorcontrib><creatorcontrib>Ramazanoglu, Mustafa</creatorcontrib><title>Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>•Mesenchymal stem cells from human tooth germs (hTGSCs) were successfully isolated.•HTGSCs showed a high osteogenic capacity.•STRO-1+ cells were selected to obtain a homogenous subpopulation of hTGSCs.•STRO-1+ subpopulation did not show a superior behavior for mineralization.
Mesenchymal stem cells derived from the human tooth germ (hTGSCs) are a heterogeneous cell population that can differentiate into osteogenic, neurogenic, and adipogenic lineages. The aim of this study was to compare the osteogenic differentiation capacity of STRO-1 positive (STRO-1+) hTGSCs and unsorted heterogeneous hTGSCs and to establish if STRO-1+ cells are more committed to osteogenic differentiation. HTGSCs were isolated from impacted third molar tooth germ tissues of adolescents, and a subpopulation of STRO-1+ hTGSCs was obtained by fluorescence-activated cell sorting. STRO-1+, STRO-1 negative (STRO-1−), and unsorted cells were cultured in osteogenic and standard culture media to compare their capacity to differentiate towards osteoblastic lineage. Cells were tested for proliferation rates, alkaline phosphatase activity, and amounts of accumulated calcium. Gene expression levels of the RUNX2, osteocalcin, and osteonectin genes were analyzed with real time PCR. Mineralization and osteogenic protein expression were examined by using von Kossa staining and confocal microscopy. Our results indicated that osteogenically induced cell populations showed greater mineralization capacity than non-induced cells. However, expression levels of early and late osteogenic markers were not significantly different between STRO-1+ and unsorted cells. In conclusion, the selection by STRO-1 expression does not yield cells with osteogenic capacity higher than that of the heterogeneous hTGSC population. Cell sorting using osteogenic markers other than STRO-1 might be beneficial in obtaining a more sensitive osteogenic sub-population from unsorted heterogenous hTGSCs.</description><subject>Adolescent</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Antigens, Surface - physiology</subject><subject>Calcium - analysis</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Male</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Microscopy, Confocal</subject><subject>Molar, Third</subject><subject>Osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>STRO-1</subject><subject>Tooth germ</subject><subject>Tooth Germ - cytology</subject><subject>Young Adult</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFtL5TAURoM46PHyF4b45ktr0ksuj3IYZwRBUOc5pMmOzaFtziSt4L835TjDPAqBTcL69s5eCF1RUlJC2c2u1NH0Ieqh86GsCOUlYSWpxBHaUMFlQVvCjtGGEFIXUjJ5is5S2uVryxg9QaeVYIJJ0WxQfz-5YYHJAA4OP788PRYUJxjAzD5MeD1phvAKkzd4H2aYZq-Hle2XUU94DmHu8SvEEVuI_g0sHiHlfv37mLmcHbGBYUgX6JvTQ4LLz3qOft_9eNn-Kh4ef95vbx8KU3M-F40GwaHTLfCOssY5WTtw0GhbaZdfu7YxlbXMtbyuGmOYaDsQ0ra8MsJyXZ-j60PffQx_FkizGn1af6AnCEtSVFJes0rKNqPygJoYUorg1D76Ucd3RYlaRaud-k-0WkUrwlQWnbPfP8cs3Qj2X_Kv2QxsDwDkZd88RJWMXz1bH7NcZYP_wpgPmj6X3Q</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Ercal, Pinar</creator><creator>Pekozer, Gorke G.</creator><creator>Gumru, Osman Z.</creator><creator>Kose, Gamze T.</creator><creator>Ramazanoglu, Mustafa</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201710</creationdate><title>Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells</title><author>Ercal, Pinar ; Pekozer, Gorke G. ; Gumru, Osman Z. ; Kose, Gamze T. ; Ramazanoglu, Mustafa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-4ae87eba5e7b164ff93fefe4ad2afa5eb54c2dd6f57324cc685be89d572c8d7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Antigens, Surface - physiology</topic><topic>Calcium - analysis</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Male</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Microscopy, Confocal</topic><topic>Molar, Third</topic><topic>Osteogenesis</topic><topic>Osteogenesis - physiology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>STRO-1</topic><topic>Tooth germ</topic><topic>Tooth Germ - cytology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ercal, Pinar</creatorcontrib><creatorcontrib>Pekozer, Gorke G.</creatorcontrib><creatorcontrib>Gumru, Osman Z.</creatorcontrib><creatorcontrib>Kose, Gamze T.</creatorcontrib><creatorcontrib>Ramazanoglu, Mustafa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ercal, Pinar</au><au>Pekozer, Gorke G.</au><au>Gumru, Osman Z.</au><au>Kose, Gamze T.</au><au>Ramazanoglu, Mustafa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2017-10</date><risdate>2017</risdate><volume>82</volume><spage>293</spage><epage>301</epage><pages>293-301</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>•Mesenchymal stem cells from human tooth germs (hTGSCs) were successfully isolated.•HTGSCs showed a high osteogenic capacity.•STRO-1+ cells were selected to obtain a homogenous subpopulation of hTGSCs.•STRO-1+ subpopulation did not show a superior behavior for mineralization.
Mesenchymal stem cells derived from the human tooth germ (hTGSCs) are a heterogeneous cell population that can differentiate into osteogenic, neurogenic, and adipogenic lineages. The aim of this study was to compare the osteogenic differentiation capacity of STRO-1 positive (STRO-1+) hTGSCs and unsorted heterogeneous hTGSCs and to establish if STRO-1+ cells are more committed to osteogenic differentiation. HTGSCs were isolated from impacted third molar tooth germ tissues of adolescents, and a subpopulation of STRO-1+ hTGSCs was obtained by fluorescence-activated cell sorting. STRO-1+, STRO-1 negative (STRO-1−), and unsorted cells were cultured in osteogenic and standard culture media to compare their capacity to differentiate towards osteoblastic lineage. Cells were tested for proliferation rates, alkaline phosphatase activity, and amounts of accumulated calcium. Gene expression levels of the RUNX2, osteocalcin, and osteonectin genes were analyzed with real time PCR. Mineralization and osteogenic protein expression were examined by using von Kossa staining and confocal microscopy. Our results indicated that osteogenically induced cell populations showed greater mineralization capacity than non-induced cells. However, expression levels of early and late osteogenic markers were not significantly different between STRO-1+ and unsorted cells. In conclusion, the selection by STRO-1 expression does not yield cells with osteogenic capacity higher than that of the heterogeneous hTGSC population. Cell sorting using osteogenic markers other than STRO-1 might be beneficial in obtaining a more sensitive osteogenic sub-population from unsorted heterogenous hTGSCs.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28686984</pmid><doi>10.1016/j.archoralbio.2017.06.028</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9969 |
| ispartof | Archives of oral biology, 2017-10, Vol.82, p.293-301 |
| issn | 0003-9969 1879-1506 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1917362995 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adolescent Alkaline Phosphatase - analysis Antigens, Surface - physiology Calcium - analysis Cell Differentiation Cell Proliferation Cells, Cultured Dentistry Female Flow Cytometry Humans Male Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Microscopy, Confocal Molar, Third Osteogenesis Osteogenesis - physiology Real-Time Polymerase Chain Reaction STRO-1 Tooth germ Tooth Germ - cytology Young Adult |
| title | Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T03%3A10%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20STRO-1%20selection%20on%20osteogenic%20potential%20of%20human%20tooth%20germ%20derived%20mesenchymal%20stem%20cells&rft.jtitle=Archives%20of%20oral%20biology&rft.au=Ercal,%20Pinar&rft.date=2017-10&rft.volume=82&rft.spage=293&rft.epage=301&rft.pages=293-301&rft.issn=0003-9969&rft.eissn=1879-1506&rft_id=info:doi/10.1016/j.archoralbio.2017.06.028&rft_dat=%3Cproquest_cross%3E1917362995%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1917362995&rft_id=info:pmid/28686984&rft_els_id=S0003996917302054&rfr_iscdi=true |