Use of luciferase probes to measure ATP in living cells and animals

This protocol describes how to construct luciferase probes that are targeted to the mitochondrial matrix or the outer surface of the plasma membrane. These probes can be used to measure ATP concentrations in different cellular compartments. ATP, the energy exchange factor that connects anabolism and...

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Veröffentlicht in:	Nature protocols 2017-08, Vol.12 (8), p.1542-1562
Hauptverfasser:	Morciano, Giampaolo, Sarti, Alba Clara, Marchi, Saverio, Missiroli, Sonia, Falzoni, Simonetta, Raffaghello, Lizzia, Pistoia, Vito, Giorgi, Carlotta, Di Virgilio, Francesco, Pinton, Paolo
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888 631/1647/245/2222 631/1647/767 631/443/319/333 Active transport Adenosine Triphosphate - analysis Analysis Analytical Chemistry Animals Apoptosis ATP ATP (Adenosine triphosphate) Biological Techniques Bioluminescence Biosensing Techniques - methods Catabolism Cell growth Cell Line Cells (biology) Chimeras Computational Biology/Bioinformatics Cytological Techniques - methods Cytosol Energy measurement Enzymes Fluorescent indicators Humans Inflammation Life Sciences Light emission Localization Luciferase Luciferases - metabolism Luciferin Luminescence Luminescent Agents - metabolism Magnesium Measurement Membranes Metabolism Mice Microarrays Mitochondria Muscle contraction Muscles Muscular function Organic Chemistry Oxidation Phosphorylation Plasma Probes Proteins Protocol Risk factors Staining and Labeling - methods Substrates
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