Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies
A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV...
Gespeichert in:
| Veröffentlicht in: | Biomedical chromatography 2018-02, Vol.32 (2), p.n/a |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 2 |
| container_start_page | |
| container_title | Biomedical chromatography |
| container_volume | 32 |
| creator | Hidau, Mahendra Kumar Kolluru, Srikanth Palakurthi, Srinath |
| description | A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile (65:35) as the precipitating solvent. The method proved to be sensitive and reproducible over a 100–2000 ng/mL linearity range with a lower limit of quantification (LLQC) of 100 ng/mL. TZP recovery was found to be >85%. Following analytical method development and validation, it was successfully employed to determine the plasma protein binding of TZP. TZP has a high level of protein binding in rat plasma (95.76 ± 0.38%) as determined by dialysis method. |
| doi_str_mv | 10.1002/bmc.4046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1916380337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1916380337</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3876-fcb9a4f60941cda4d2d83fb85b2e47df12cb1789c3d062656a1bc432312eb3253</originalsourceid><addsrcrecordid>eNp1kctu1DAUQC1ERYeCxBcgL9mk9SPjxOzKUChSKzawjq5fEyMnzthO0bDiE_otfBJfQkqnZcXqSldHR_fqIPSKklNKCDtTgz6tSS2eoBUlUlakJfQpWhEmZMXbRh6j5zl_I4RIwZpn6Ji1omlaRlfo13t7Y0OcBjsWDKPBNxC8geLjiKPDgHu_7X__vJ1scjENMGqLg9_N3mDdpzhAidsEU7_Hgy19NHihcOkt3s0wFu-8fnQVCPAjTpC8wn7ECQqeAuQB3uLzaQoPZImHNZ5SLHYhlR-NH7c4l9l4m1-gIwch25eHeYK-frj4srmsrj5__LQ5v6r08rKonFYSaieIrKk2UBtmWu5Uu1bM1o1xlGlFm1ZqbohgYi2AKl1zximzirM1P0Fv7r3LHbvZ5tINPmsbAow2zrmjkgreEs6bf6hOMedkXTclP0Dad5R0d4W6pVB3V2hBXx-ssxqseQQfkixAdQ9898Hu_yvq3l1v_gr_AB6Bn8A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1916380337</pqid></control><display><type>article</type><title>Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Hidau, Mahendra Kumar ; Kolluru, Srikanth ; Palakurthi, Srinath</creator><creatorcontrib>Hidau, Mahendra Kumar ; Kolluru, Srikanth ; Palakurthi, Srinath</creatorcontrib><description>A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile (65:35) as the precipitating solvent. The method proved to be sensitive and reproducible over a 100–2000 ng/mL linearity range with a lower limit of quantification (LLQC) of 100 ng/mL. TZP recovery was found to be >85%. Following analytical method development and validation, it was successfully employed to determine the plasma protein binding of TZP. TZP has a high level of protein binding in rat plasma (95.76 ± 0.38%) as determined by dialysis method.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.4046</identifier><identifier>PMID: 28677821</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Blood Proteins - analysis ; Blood Proteins - chemistry ; Blood Proteins - metabolism ; chromatography ; Chromatography, High Pressure Liquid - methods ; Chromatography, Reverse-Phase ; Drug Stability ; Linear Models ; PARP inhibitor ; Phthalazines - blood ; Phthalazines - chemistry ; Phthalazines - metabolism ; Protein Binding ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Biomedical chromatography, 2018-02, Vol.32 (2), p.n/a</ispartof><rights>Copyright © 2017 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3876-fcb9a4f60941cda4d2d83fb85b2e47df12cb1789c3d062656a1bc432312eb3253</citedby><cites>FETCH-LOGICAL-c3876-fcb9a4f60941cda4d2d83fb85b2e47df12cb1789c3d062656a1bc432312eb3253</cites><orcidid>0000-0002-9509-9511 ; 0000-0003-1455-5392</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.4046$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.4046$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28677821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hidau, Mahendra Kumar</creatorcontrib><creatorcontrib>Kolluru, Srikanth</creatorcontrib><creatorcontrib>Palakurthi, Srinath</creatorcontrib><title>Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies</title><title>Biomedical chromatography</title><addtitle>Biomed Chromatogr</addtitle><description>A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile (65:35) as the precipitating solvent. The method proved to be sensitive and reproducible over a 100–2000 ng/mL linearity range with a lower limit of quantification (LLQC) of 100 ng/mL. TZP recovery was found to be >85%. Following analytical method development and validation, it was successfully employed to determine the plasma protein binding of TZP. TZP has a high level of protein binding in rat plasma (95.76 ± 0.38%) as determined by dialysis method.</description><subject>Animals</subject><subject>Blood Proteins - analysis</subject><subject>Blood Proteins - chemistry</subject><subject>Blood Proteins - metabolism</subject><subject>chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Reverse-Phase</subject><subject>Drug Stability</subject><subject>Linear Models</subject><subject>PARP inhibitor</subject><subject>Phthalazines - blood</subject><subject>Phthalazines - chemistry</subject><subject>Phthalazines - metabolism</subject><subject>Protein Binding</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUQC1ERYeCxBcgL9mk9SPjxOzKUChSKzawjq5fEyMnzthO0bDiE_otfBJfQkqnZcXqSldHR_fqIPSKklNKCDtTgz6tSS2eoBUlUlakJfQpWhEmZMXbRh6j5zl_I4RIwZpn6Ji1omlaRlfo13t7Y0OcBjsWDKPBNxC8geLjiKPDgHu_7X__vJ1scjENMGqLg9_N3mDdpzhAidsEU7_Hgy19NHihcOkt3s0wFu-8fnQVCPAjTpC8wn7ECQqeAuQB3uLzaQoPZImHNZ5SLHYhlR-NH7c4l9l4m1-gIwch25eHeYK-frj4srmsrj5__LQ5v6r08rKonFYSaieIrKk2UBtmWu5Uu1bM1o1xlGlFm1ZqbohgYi2AKl1zximzirM1P0Fv7r3LHbvZ5tINPmsbAow2zrmjkgreEs6bf6hOMedkXTclP0Dad5R0d4W6pVB3V2hBXx-ssxqseQQfkixAdQ9898Hu_yvq3l1v_gr_AB6Bn8A</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Hidau, Mahendra Kumar</creator><creator>Kolluru, Srikanth</creator><creator>Palakurthi, Srinath</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9509-9511</orcidid><orcidid>https://orcid.org/0000-0003-1455-5392</orcidid></search><sort><creationdate>201802</creationdate><title>Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies</title><author>Hidau, Mahendra Kumar ; Kolluru, Srikanth ; Palakurthi, Srinath</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3876-fcb9a4f60941cda4d2d83fb85b2e47df12cb1789c3d062656a1bc432312eb3253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Blood Proteins - analysis</topic><topic>Blood Proteins - chemistry</topic><topic>Blood Proteins - metabolism</topic><topic>chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Reverse-Phase</topic><topic>Drug Stability</topic><topic>Linear Models</topic><topic>PARP inhibitor</topic><topic>Phthalazines - blood</topic><topic>Phthalazines - chemistry</topic><topic>Phthalazines - metabolism</topic><topic>Protein Binding</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hidau, Mahendra Kumar</creatorcontrib><creatorcontrib>Kolluru, Srikanth</creatorcontrib><creatorcontrib>Palakurthi, Srinath</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hidau, Mahendra Kumar</au><au>Kolluru, Srikanth</au><au>Palakurthi, Srinath</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed Chromatogr</addtitle><date>2018-02</date><risdate>2018</risdate><volume>32</volume><issue>2</issue><epage>n/a</epage><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile (65:35) as the precipitating solvent. The method proved to be sensitive and reproducible over a 100–2000 ng/mL linearity range with a lower limit of quantification (LLQC) of 100 ng/mL. TZP recovery was found to be >85%. Following analytical method development and validation, it was successfully employed to determine the plasma protein binding of TZP. TZP has a high level of protein binding in rat plasma (95.76 ± 0.38%) as determined by dialysis method.</abstract><cop>England</cop><pmid>28677821</pmid><doi>10.1002/bmc.4046</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9509-9511</orcidid><orcidid>https://orcid.org/0000-0003-1455-5392</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-3879 |
| ispartof | Biomedical chromatography, 2018-02, Vol.32 (2), p.n/a |
| issn | 0269-3879 1099-0801 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1916380337 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Blood Proteins - analysis Blood Proteins - chemistry Blood Proteins - metabolism chromatography Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase Drug Stability Linear Models PARP inhibitor Phthalazines - blood Phthalazines - chemistry Phthalazines - metabolism Protein Binding Rats Rats, Sprague-Dawley Reproducibility of Results Sensitivity and Specificity |
| title | Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T10%3A26%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20validation%20of%20a%20high%E2%80%90performance%20liquid%20chromatography%20method%20for%20the%20quantification%20of%20talazoparib%20in%20rat%20plasma:%20Application%20to%20plasma%20protein%20binding%20studies&rft.jtitle=Biomedical%20chromatography&rft.au=Hidau,%20Mahendra%20Kumar&rft.date=2018-02&rft.volume=32&rft.issue=2&rft.epage=n/a&rft.issn=0269-3879&rft.eissn=1099-0801&rft_id=info:doi/10.1002/bmc.4046&rft_dat=%3Cproquest_cross%3E1916380337%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1916380337&rft_id=info:pmid/28677821&rfr_iscdi=true |