Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia
We have characterized in this study 10 PPMV-1 isolated from domestic pigeons and one PPMV-1 isolated from a feral pigeon in the period 2007–2012, using both classical methods (HI test and ICPI test) and molecular methods (RT-qPCR, RT-PCR, and nucleotide sequencing). Using phylogenetic analysis of pa...
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| Veröffentlicht in: | Avian diseases 2017-06, Vol.61 (2), p.146-152 |
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| creator | Dodovski, A Cvetkovikj, I Krstevski, K Naletoski, I Savić, Vladimir |
| description | We have characterized in this study 10 PPMV-1 isolated from domestic pigeons and one PPMV-1 isolated from a feral pigeon in the period 2007–2012, using both classical methods (HI test and ICPI test) and molecular methods (RT-qPCR, RT-PCR, and nucleotide sequencing). Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53. The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of different isolates in the same holdings was detected. This is the first study to extensively describe the molecular epidemiology of PPMV-1 isolated in Macedonia. |
| doi_str_mv | 10.1637/11517-101816-Reg.1 |
| format | Article |
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Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53. The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of different isolates in the same holdings was detected. 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Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53. The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of different isolates in the same holdings was detected. This is the first study to extensively describe the molecular epidemiology of PPMV-1 isolated in Macedonia.</description><subject>Animals</subject><subject>Bird Diseases - epidemiology</subject><subject>Bird Diseases - virology</subject><subject>characterization</subject><subject>Columbidae - virology</subject><subject>epidemiology</subject><subject>Genotype</subject><subject>Macedonia</subject><subject>Macedonia (Republic) - epidemiology</subject><subject>Paramyxoviridae - classification</subject><subject>Paramyxoviridae - genetics</subject><subject>Paramyxoviridae - isolation & purification</subject><subject>Paramyxoviridae Infections - epidemiology</subject><subject>Paramyxoviridae Infections - veterinary</subject><subject>Paramyxoviridae Infections - virology</subject><subject>Phylogeny</subject><subject>pigeon</subject><subject>pigeon paramyxovirus type 1</subject><subject>Regular s</subject><issn>0005-2086</issn><issn>1938-4351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9P3DAQxa0KVJZtv0ClVj7CwXQmTpzkWK1oQWLVVbXlajnJZGuUxFs7i1g-PYbw58ppNHrvzej9GPuCcIZK5t8RM8wFAhaoxB_anOEHNsNSFiKVGR6wGQBkIoFCHbHjEG4AMC8VfGRHSaFUlif5jG0W_4w39Uje3pvRuoGboeHnW9tQb13nNnvuWr6yG4rSKlr7_Z27tX4X-Hq_JYH8-nGhwE9Wq-W1wFN-GVxnRmq4HfjS1NS4wZpP7LA1XaDPz3PO_v48Xy8uxNXvX5eLH1eikkqNgmSJpkoLCSihSttYRGFClWmTDJWp66qODdKsggKAFJTQlqYxsaMkautUztnJdHfr3f8dhVH3NtTUdWYgtwsaS8xkWsr4Ys6SyVp7F4KnVm-97Y3fawT9CFg_AdYTYB0Ba4yhb8_3d1VPzWvkhWg0fJ0MN2F0_k1XqUywhKjDpFfWuYHe8_MBq8aOug</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Dodovski, A</creator><creator>Cvetkovikj, I</creator><creator>Krstevski, K</creator><creator>Naletoski, I</creator><creator>Savić, Vladimir</creator><general>American Association of Avian Pathologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia</title><author>Dodovski, A ; Cvetkovikj, I ; Krstevski, K ; Naletoski, I ; Savić, Vladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b366t-e391ab4830130b4f435612ebaf2516accbc96045b0800e6090f9ada2083eefc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bird Diseases - epidemiology</topic><topic>Bird Diseases - virology</topic><topic>characterization</topic><topic>Columbidae - virology</topic><topic>epidemiology</topic><topic>Genotype</topic><topic>Macedonia</topic><topic>Macedonia (Republic) - epidemiology</topic><topic>Paramyxoviridae - classification</topic><topic>Paramyxoviridae - genetics</topic><topic>Paramyxoviridae - isolation & purification</topic><topic>Paramyxoviridae Infections - epidemiology</topic><topic>Paramyxoviridae Infections - veterinary</topic><topic>Paramyxoviridae Infections - virology</topic><topic>Phylogeny</topic><topic>pigeon</topic><topic>pigeon paramyxovirus type 1</topic><topic>Regular s</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dodovski, A</creatorcontrib><creatorcontrib>Cvetkovikj, I</creatorcontrib><creatorcontrib>Krstevski, K</creatorcontrib><creatorcontrib>Naletoski, I</creatorcontrib><creatorcontrib>Savić, Vladimir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Avian diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dodovski, A</au><au>Cvetkovikj, I</au><au>Krstevski, K</au><au>Naletoski, I</au><au>Savić, Vladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia</atitle><jtitle>Avian diseases</jtitle><addtitle>Avian Dis</addtitle><date>2017-06</date><risdate>2017</risdate><volume>61</volume><issue>2</issue><spage>146</spage><epage>152</epage><pages>146-152</pages><issn>0005-2086</issn><eissn>1938-4351</eissn><abstract>We have characterized in this study 10 PPMV-1 isolated from domestic pigeons and one PPMV-1 isolated from a feral pigeon in the period 2007–2012, using both classical methods (HI test and ICPI test) and molecular methods (RT-qPCR, RT-PCR, and nucleotide sequencing). Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53. The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of different isolates in the same holdings was detected. This is the first study to extensively describe the molecular epidemiology of PPMV-1 isolated in Macedonia.</abstract><cop>United States</cop><pub>American Association of Avian Pathologists</pub><pmid>28665727</pmid><doi>10.1637/11517-101816-Reg.1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Avian diseases, 2017-06, Vol.61 (2), p.146-152 |
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| subjects | Animals Bird Diseases - epidemiology Bird Diseases - virology characterization Columbidae - virology epidemiology Genotype Macedonia Macedonia (Republic) - epidemiology Paramyxoviridae - classification Paramyxoviridae - genetics Paramyxoviridae - isolation & purification Paramyxoviridae Infections - epidemiology Paramyxoviridae Infections - veterinary Paramyxoviridae Infections - virology Phylogeny pigeon pigeon paramyxovirus type 1 Regular s |
| title | Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia |
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