Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution
Puumala virus (PUUV), carried by bank voles ( Myodes glareolus ), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed fo...
Gespeichert in:
| Veröffentlicht in: | Virus genes 2017-12, Vol.53 (6), p.913-917 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 917 |
|---|---|
| container_issue | 6 |
| container_start_page | 913 |
| container_title | Virus genes |
| container_volume | 53 |
| creator | Szabó, Róbert Radosa, Lukáš Ličková, Martina Sláviková, Monika Heroldová, Marta Stanko, Michal Pejčoch, Milan Osterberg, Anja Laenen, Lies Schex, Susanne Ulrich, Rainer G. Essbauer, Sandra Maes, Piet Klempa, Boris |
| description | Puumala virus (PUUV), carried by bank voles (
Myodes glareolus
), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from Šumava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases. |
| doi_str_mv | 10.1007/s11262-017-1484-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1915342318</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1966808091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-a1c8eaba7ade58b8e44e2d33edd1e6dcecb22930527c401b1f39591c6492dba63</originalsourceid><addsrcrecordid>eNp1kc2K3SAYQKW0dG6nfYBuitBNN2nVGKPLcpn-wEBnMQPdBWO-3DgYTTVeuO8wD10vmZZSmIWIeL4jeBB6S8lHSkj7KVHKBKsIbSvKJa-aZ2hHm5ZVSvGfz9GOKEYq2Qh1gV6ldE8IkZLxl-iCSSE4F-0OPdxMJxcO4GG1Bmuv3SnZhMOIb3KetdP4aGNOOK1RW5_wGMOM9-DL0eGrHMMCeLKHyZW1JrxOgD3AgMcQscZjdq4q8jADXiCmBcxqj4CDx5P2q97ccAwurzb41-jFqF2CN4_7Jbr7cnW7_1Zd__j6ff_5ujJ1y9ZKUyNB97rVAzSyl8A5sKGuYRgoiMGA6RlTNWlYazihPR1r1ShqBFds6LWoL9GHzbvE8CtDWrvZJgPOaQ8hp44q2tSc1VQW9P1_6H3IsXzTmRJCEkkULRTdKBNDShHGbol21vHUUdKdU3Vbqq6k6s6puqbMvHs0536G4e_EnzYFYBuQypU_QPzn6SetvwG3B6IZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1966808091</pqid></control><display><type>article</type><title>Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Szabó, Róbert ; Radosa, Lukáš ; Ličková, Martina ; Sláviková, Monika ; Heroldová, Marta ; Stanko, Michal ; Pejčoch, Milan ; Osterberg, Anja ; Laenen, Lies ; Schex, Susanne ; Ulrich, Rainer G. ; Essbauer, Sandra ; Maes, Piet ; Klempa, Boris</creator><creatorcontrib>Szabó, Róbert ; Radosa, Lukáš ; Ličková, Martina ; Sláviková, Monika ; Heroldová, Marta ; Stanko, Michal ; Pejčoch, Milan ; Osterberg, Anja ; Laenen, Lies ; Schex, Susanne ; Ulrich, Rainer G. ; Essbauer, Sandra ; Maes, Piet ; Klempa, Boris</creatorcontrib><description>Puumala virus (PUUV), carried by bank voles (
Myodes glareolus
), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from Šumava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-017-1484-5</identifier><identifier>PMID: 28664467</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Arvicolinae - virology ; Biomedical and Life Sciences ; Biomedicine ; Czech Republic ; Europe ; Evolution, Molecular ; Genomes ; Genotype ; Germany ; Hantavirus ; Hantavirus - genetics ; Hantavirus Infections - virology ; Homologous recombination ; Humans ; Medical Microbiology ; Outbreaks ; Phylogenetics ; Phylogeny ; Plant Sciences ; Puumala virus - genetics ; Ribonucleic acid ; RNA ; RNA, Viral - genetics ; Rodents ; Slovakia ; Strains (organisms) ; Virology</subject><ispartof>Virus genes, 2017-12, Vol.53 (6), p.913-917</ispartof><rights>Springer Science+Business Media, LLC 2017</rights><rights>Virus Genes is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-a1c8eaba7ade58b8e44e2d33edd1e6dcecb22930527c401b1f39591c6492dba63</citedby><cites>FETCH-LOGICAL-c372t-a1c8eaba7ade58b8e44e2d33edd1e6dcecb22930527c401b1f39591c6492dba63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-017-1484-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-017-1484-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27906,27907,41470,42539,51301</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28664467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Szabó, Róbert</creatorcontrib><creatorcontrib>Radosa, Lukáš</creatorcontrib><creatorcontrib>Ličková, Martina</creatorcontrib><creatorcontrib>Sláviková, Monika</creatorcontrib><creatorcontrib>Heroldová, Marta</creatorcontrib><creatorcontrib>Stanko, Michal</creatorcontrib><creatorcontrib>Pejčoch, Milan</creatorcontrib><creatorcontrib>Osterberg, Anja</creatorcontrib><creatorcontrib>Laenen, Lies</creatorcontrib><creatorcontrib>Schex, Susanne</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><creatorcontrib>Essbauer, Sandra</creatorcontrib><creatorcontrib>Maes, Piet</creatorcontrib><creatorcontrib>Klempa, Boris</creatorcontrib><title>Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Puumala virus (PUUV), carried by bank voles (
Myodes glareolus
), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from Šumava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases.</description><subject>Animals</subject><subject>Arvicolinae - virology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Czech Republic</subject><subject>Europe</subject><subject>Evolution, Molecular</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Germany</subject><subject>Hantavirus</subject><subject>Hantavirus - genetics</subject><subject>Hantavirus Infections - virology</subject><subject>Homologous recombination</subject><subject>Humans</subject><subject>Medical Microbiology</subject><subject>Outbreaks</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Puumala virus - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>Rodents</subject><subject>Slovakia</subject><subject>Strains (organisms)</subject><subject>Virology</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc2K3SAYQKW0dG6nfYBuitBNN2nVGKPLcpn-wEBnMQPdBWO-3DgYTTVeuO8wD10vmZZSmIWIeL4jeBB6S8lHSkj7KVHKBKsIbSvKJa-aZ2hHm5ZVSvGfz9GOKEYq2Qh1gV6ldE8IkZLxl-iCSSE4F-0OPdxMJxcO4GG1Bmuv3SnZhMOIb3KetdP4aGNOOK1RW5_wGMOM9-DL0eGrHMMCeLKHyZW1JrxOgD3AgMcQscZjdq4q8jADXiCmBcxqj4CDx5P2q97ccAwurzb41-jFqF2CN4_7Jbr7cnW7_1Zd__j6ff_5ujJ1y9ZKUyNB97rVAzSyl8A5sKGuYRgoiMGA6RlTNWlYazihPR1r1ShqBFds6LWoL9GHzbvE8CtDWrvZJgPOaQ8hp44q2tSc1VQW9P1_6H3IsXzTmRJCEkkULRTdKBNDShHGbol21vHUUdKdU3Vbqq6k6s6puqbMvHs0536G4e_EnzYFYBuQypU_QPzn6SetvwG3B6IZ</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Szabó, Róbert</creator><creator>Radosa, Lukáš</creator><creator>Ličková, Martina</creator><creator>Sláviková, Monika</creator><creator>Heroldová, Marta</creator><creator>Stanko, Michal</creator><creator>Pejčoch, Milan</creator><creator>Osterberg, Anja</creator><creator>Laenen, Lies</creator><creator>Schex, Susanne</creator><creator>Ulrich, Rainer G.</creator><creator>Essbauer, Sandra</creator><creator>Maes, Piet</creator><creator>Klempa, Boris</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution</title><author>Szabó, Róbert ; Radosa, Lukáš ; Ličková, Martina ; Sláviková, Monika ; Heroldová, Marta ; Stanko, Michal ; Pejčoch, Milan ; Osterberg, Anja ; Laenen, Lies ; Schex, Susanne ; Ulrich, Rainer G. ; Essbauer, Sandra ; Maes, Piet ; Klempa, Boris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-a1c8eaba7ade58b8e44e2d33edd1e6dcecb22930527c401b1f39591c6492dba63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Arvicolinae - virology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Czech Republic</topic><topic>Europe</topic><topic>Evolution, Molecular</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Germany</topic><topic>Hantavirus</topic><topic>Hantavirus - genetics</topic><topic>Hantavirus Infections - virology</topic><topic>Homologous recombination</topic><topic>Humans</topic><topic>Medical Microbiology</topic><topic>Outbreaks</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Puumala virus - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>Rodents</topic><topic>Slovakia</topic><topic>Strains (organisms)</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szabó, Róbert</creatorcontrib><creatorcontrib>Radosa, Lukáš</creatorcontrib><creatorcontrib>Ličková, Martina</creatorcontrib><creatorcontrib>Sláviková, Monika</creatorcontrib><creatorcontrib>Heroldová, Marta</creatorcontrib><creatorcontrib>Stanko, Michal</creatorcontrib><creatorcontrib>Pejčoch, Milan</creatorcontrib><creatorcontrib>Osterberg, Anja</creatorcontrib><creatorcontrib>Laenen, Lies</creatorcontrib><creatorcontrib>Schex, Susanne</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><creatorcontrib>Essbauer, Sandra</creatorcontrib><creatorcontrib>Maes, Piet</creatorcontrib><creatorcontrib>Klempa, Boris</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szabó, Róbert</au><au>Radosa, Lukáš</au><au>Ličková, Martina</au><au>Sláviková, Monika</au><au>Heroldová, Marta</au><au>Stanko, Michal</au><au>Pejčoch, Milan</au><au>Osterberg, Anja</au><au>Laenen, Lies</au><au>Schex, Susanne</au><au>Ulrich, Rainer G.</au><au>Essbauer, Sandra</au><au>Maes, Piet</au><au>Klempa, Boris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>53</volume><issue>6</issue><spage>913</spage><epage>917</epage><pages>913-917</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Puumala virus (PUUV), carried by bank voles (
Myodes glareolus
), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from Šumava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28664467</pmid><doi>10.1007/s11262-017-1484-5</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-8569 |
| ispartof | Virus genes, 2017-12, Vol.53 (6), p.913-917 |
| issn | 0920-8569 1572-994X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1915342318 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Arvicolinae - virology Biomedical and Life Sciences Biomedicine Czech Republic Europe Evolution, Molecular Genomes Genotype Germany Hantavirus Hantavirus - genetics Hantavirus Infections - virology Homologous recombination Humans Medical Microbiology Outbreaks Phylogenetics Phylogeny Plant Sciences Puumala virus - genetics Ribonucleic acid RNA RNA, Viral - genetics Rodents Slovakia Strains (organisms) Virology |
| title | Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T09%3A19%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phylogenetic%20analysis%20of%20Puumala%20virus%20strains%20from%20Central%20Europe%20highlights%20the%20need%20for%20a%20full-genome%20perspective%20on%20hantavirus%20evolution&rft.jtitle=Virus%20genes&rft.au=Szab%C3%B3,%20R%C3%B3bert&rft.date=2017-12-01&rft.volume=53&rft.issue=6&rft.spage=913&rft.epage=917&rft.pages=913-917&rft.issn=0920-8569&rft.eissn=1572-994X&rft_id=info:doi/10.1007/s11262-017-1484-5&rft_dat=%3Cproquest_cross%3E1966808091%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1966808091&rft_id=info:pmid/28664467&rfr_iscdi=true |