Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold
ABSTRACT Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaff...
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| Veröffentlicht in: | The FASEB journal 2017-10, Vol.31 (10), p.4555-4565 |
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| creator | Mazzoni, Elisa D’Agostino, Antonio Manfrini, Marco Maniero, Stefania Puozzo, Andrea Bassi, Elena Marsico, Stefano Fortini, Cinzia Trevisiol, Lorenzo Patergnani, Simone Tognon, Mauro |
| description | ABSTRACT
Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up‐regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our in vitro data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate in vitro bone induction.—Mazzoni, E., D’Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold. FASEB J. 31, 4555–4565 (2017). www.fasebj.org |
| doi_str_mv | 10.1096/fj.201601384R |
| format | Article |
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Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up‐regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our in vitro data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate in vitro bone induction.—Mazzoni, E., D’Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold. FASEB J. 31, 4555–4565 (2017). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.201601384R</identifier><identifier>PMID: 28659417</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Adhesion ; Adipocytes - cytology ; Adipocytes - drug effects ; Adipocytes - metabolism ; Adipose Tissue - cytology ; Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; Biocompatibility ; Biocompatible Materials - pharmacology ; biomaterial ; Biomaterials ; Biomedical materials ; bone ; Bone and Bones - drug effects ; Bone and Bones - metabolism ; Bone biomaterials ; Cell Differentiation - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cells, Cultured ; Collagen ; Collagen - metabolism ; Differentiation ; Extracellular matrix ; Extracellular Matrix - metabolism ; Focal adhesion kinase ; Gene expression ; Genes ; Humans ; Hydroxyapatite ; Integrins ; Kinases ; matrix ; Osteoconduction ; Osteogenesis - drug effects ; Osteopontin ; Regrowth ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Studies ; Tissue Scaffolds</subject><ispartof>The FASEB journal, 2017-10, Vol.31 (10), p.4555-4565</ispartof><rights>FASEB</rights><rights>FASEB.</rights><rights>Copyright Federation of American Societies for Experimental Biology (FASEB) Oct 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417R-50175dae40c7db037213b06526e2efdb3ac0155d3fe6f2b75d4879da028672b73</citedby><cites>FETCH-LOGICAL-c417R-50175dae40c7db037213b06526e2efdb3ac0155d3fe6f2b75d4879da028672b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.201601384R$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.201601384R$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28659417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazzoni, Elisa</creatorcontrib><creatorcontrib>D’Agostino, Antonio</creatorcontrib><creatorcontrib>Manfrini, Marco</creatorcontrib><creatorcontrib>Maniero, Stefania</creatorcontrib><creatorcontrib>Puozzo, Andrea</creatorcontrib><creatorcontrib>Bassi, Elena</creatorcontrib><creatorcontrib>Marsico, Stefano</creatorcontrib><creatorcontrib>Fortini, Cinzia</creatorcontrib><creatorcontrib>Trevisiol, Lorenzo</creatorcontrib><creatorcontrib>Patergnani, Simone</creatorcontrib><creatorcontrib>Tognon, Mauro</creatorcontrib><title>Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>ABSTRACT
Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up‐regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our in vitro data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate in vitro bone induction.—Mazzoni, E., D’Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold. FASEB J. 31, 4555–4565 (2017). www.fasebj.org</description><subject>Adhesion</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - pharmacology</subject><subject>biomaterial</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>bone</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - metabolism</subject><subject>Bone biomaterials</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Differentiation</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Focal adhesion kinase</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Integrins</subject><subject>Kinases</subject><subject>matrix</subject><subject>Osteoconduction</subject><subject>Osteogenesis - drug effects</subject><subject>Osteopontin</subject><subject>Regrowth</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Studies</subject><subject>Tissue Scaffolds</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90UFvFSEQB3BiNPZZPXo1JF68bDvALsvGkzY-q2liUvW8YWGwvOzCE3Zr99tL86ptPHgiDD_-mckQ8pLBCYNOnrrdCQcmgQlVXz4iG9YIqKSS8JhsQHW8klKoI_Is5x0AsEKfkiOuZNPVrN2Qm_Nl0oFq6_cxI80zTtTgOGbqg10MWjpHGks5_sDgDbXeOUwYZq9nHwMdVlq--xDidSlcIzVxHHWxp1erTfFmHfW-PMxIr9YheUuz0c7F0T4nT5weM764O4_J9-2Hb2fn1cWXj5_O3l1UpvR3WTXA2sZqrMG0dgDRciYGkA2XyNHZQWgDrGmscCgdH4qtVdtZDWXEttzFMXlzyN2n-HPBPPeTz7cT6oBxyT3rWK1qWTddoa__obu4pFC6K0rVQgneqaKqgzIp5pzQ9fvkJ53WnkF_u5Le7fr7lRT_6i51GSa0f_WfHRTw9gB--RHX_6f126_v-fbzg_jfBPqZyg</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Mazzoni, Elisa</creator><creator>D’Agostino, Antonio</creator><creator>Manfrini, Marco</creator><creator>Maniero, Stefania</creator><creator>Puozzo, Andrea</creator><creator>Bassi, Elena</creator><creator>Marsico, Stefano</creator><creator>Fortini, Cinzia</creator><creator>Trevisiol, Lorenzo</creator><creator>Patergnani, Simone</creator><creator>Tognon, Mauro</creator><general>Federation of American Societies for Experimental Biology</general><general>Federation of American Societies for Experimental Biology (FASEB)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201710</creationdate><title>Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold</title><author>Mazzoni, Elisa ; 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Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up‐regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our in vitro data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate in vitro bone induction.—Mazzoni, E., D’Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold. FASEB J. 31, 4555–4565 (2017). www.fasebj.org</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>28659417</pmid><doi>10.1096/fj.201601384R</doi><tpages>11</tpages></addata></record> |
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| subjects | Adhesion Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Adipose Tissue - cytology Adipose Tissue - drug effects Adipose Tissue - metabolism Biocompatibility Biocompatible Materials - pharmacology biomaterial Biomaterials Biomedical materials bone Bone and Bones - drug effects Bone and Bones - metabolism Bone biomaterials Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Cells, Cultured Collagen Collagen - metabolism Differentiation Extracellular matrix Extracellular Matrix - metabolism Focal adhesion kinase Gene expression Genes Humans Hydroxyapatite Integrins Kinases matrix Osteoconduction Osteogenesis - drug effects Osteopontin Regrowth Stem cells Stem Cells - cytology Stem Cells - drug effects Studies Tissue Scaffolds |
| title | Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold |
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