Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method
Background We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin...
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| Veröffentlicht in: | Annals of clinical biochemistry 2018-03, Vol.55 (2), p.276-280 |
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| creator | Okada, Hitoshi Kawada, Kou Itoh, Susumu Ozaki, Miyo Kakutani, Isami Arai, Takeshi Koyano, Kosuke Yasuda, Saneyuki Iwase, Takashi Murao, Koji Kusaka, Takashi |
| description | Background
We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light.
Methods
Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24 h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography.
Results
Direct bilirubin increased significantly from 0.61 to 2.36 mg/dL. The respective ratios of bilirubin photoisomers before and after exposure were as follows: cyclobilirubin (0.007 to 0.29) and (EZ)-bilirubin (0.018 to 0.041) increased significantly, (ZZ)-bilirubin decreased 0.84 to 0.55 significantly. The difference of the cyclobilirubin concentration was most closely associated with those of the direct bilirubin concentration.
Conclusion
Direct bilirubin value was increased after exposure to the room light, and increase in direct bilirubin was significantly correlated by cyclobilirubin increase in the serum samples from neonates with indirect hyperbilirubinemia. |
| doi_str_mv | 10.1177/0004563217716474 |
| format | Article |
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We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light.
Methods
Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24 h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography.
Results
Direct bilirubin increased significantly from 0.61 to 2.36 mg/dL. The respective ratios of bilirubin photoisomers before and after exposure were as follows: cyclobilirubin (0.007 to 0.29) and (EZ)-bilirubin (0.018 to 0.041) increased significantly, (ZZ)-bilirubin decreased 0.84 to 0.55 significantly. The difference of the cyclobilirubin concentration was most closely associated with those of the direct bilirubin concentration.
Conclusion
Direct bilirubin value was increased after exposure to the room light, and increase in direct bilirubin was significantly correlated by cyclobilirubin increase in the serum samples from neonates with indirect hyperbilirubinemia.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/0004563217716474</identifier><identifier>PMID: 28656812</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Bilirubin - blood ; Chromatography, High Pressure Liquid ; Humans ; Hyperbilirubinemia, Neonatal - blood ; Hyperbilirubinemia, Neonatal - therapy ; Infant, Newborn ; Isomerism ; Light ; Oxidoreductases Acting on CH-CH Group Donors - metabolism</subject><ispartof>Annals of clinical biochemistry, 2018-03, Vol.55 (2), p.276-280</ispartof><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-32936069e2dce10520e94979f9684f35c6a211afec0d4d4e07f3a2410bd44bf13</citedby><cites>FETCH-LOGICAL-c403t-32936069e2dce10520e94979f9684f35c6a211afec0d4d4e07f3a2410bd44bf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0004563217716474$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0004563217716474$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,777,781,21801,27906,27907,43603,43604</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28656812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okada, Hitoshi</creatorcontrib><creatorcontrib>Kawada, Kou</creatorcontrib><creatorcontrib>Itoh, Susumu</creatorcontrib><creatorcontrib>Ozaki, Miyo</creatorcontrib><creatorcontrib>Kakutani, Isami</creatorcontrib><creatorcontrib>Arai, Takeshi</creatorcontrib><creatorcontrib>Koyano, Kosuke</creatorcontrib><creatorcontrib>Yasuda, Saneyuki</creatorcontrib><creatorcontrib>Iwase, Takashi</creatorcontrib><creatorcontrib>Murao, Koji</creatorcontrib><creatorcontrib>Kusaka, Takashi</creatorcontrib><title>Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method</title><title>Annals of clinical biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>Background
We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light.
Methods
Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24 h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography.
Results
Direct bilirubin increased significantly from 0.61 to 2.36 mg/dL. The respective ratios of bilirubin photoisomers before and after exposure were as follows: cyclobilirubin (0.007 to 0.29) and (EZ)-bilirubin (0.018 to 0.041) increased significantly, (ZZ)-bilirubin decreased 0.84 to 0.55 significantly. The difference of the cyclobilirubin concentration was most closely associated with those of the direct bilirubin concentration.
Conclusion
Direct bilirubin value was increased after exposure to the room light, and increase in direct bilirubin was significantly correlated by cyclobilirubin increase in the serum samples from neonates with indirect hyperbilirubinemia.</description><subject>Bilirubin - blood</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Humans</subject><subject>Hyperbilirubinemia, Neonatal - blood</subject><subject>Hyperbilirubinemia, Neonatal - therapy</subject><subject>Infant, Newborn</subject><subject>Isomerism</subject><subject>Light</subject><subject>Oxidoreductases Acting on CH-CH Group Donors - metabolism</subject><issn>0004-5632</issn><issn>1758-1001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1PhDAQxRujcdfVuyfD0Qs6U0qBo9n4lWziRc-kwFTYAF1bSNz_3uKuHzHx1Gnf7710HmPnCFeISXINACKWEfczSpGIAzbHJE5DBMBDNp_kcNJn7MS5tb_yBOCYzXgqY5kin7P6VmsqBxcYHRRN29ixaPpgU5vBNM50ZL3SB0NNQUfKjZY66ocJrhrrfb88xfYT-3kw702l3GQcalOdsiOtWkdn-3PBXu5un5cP4erp_nF5swpLAdEQRjyLJMiMeFUSQsyBMpElmc5kKnQUl1JxROX_DJWoBEGiI8UFQlEJUWiMFuxyl7ux5m0kN-Rd40pqW9WTGV2OGYo4xRiFR2GHltY4Z0nnG9t0ym5zhHzqN__br7dc7NPHoqPq2_BVqAfCHeDUK-VrM9reb_t_4AdDzYLc</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Okada, Hitoshi</creator><creator>Kawada, Kou</creator><creator>Itoh, Susumu</creator><creator>Ozaki, Miyo</creator><creator>Kakutani, Isami</creator><creator>Arai, Takeshi</creator><creator>Koyano, Kosuke</creator><creator>Yasuda, Saneyuki</creator><creator>Iwase, Takashi</creator><creator>Murao, Koji</creator><creator>Kusaka, Takashi</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180301</creationdate><title>Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method</title><author>Okada, Hitoshi ; Kawada, Kou ; Itoh, Susumu ; Ozaki, Miyo ; Kakutani, Isami ; Arai, Takeshi ; Koyano, Kosuke ; Yasuda, Saneyuki ; Iwase, Takashi ; Murao, Koji ; Kusaka, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-32936069e2dce10520e94979f9684f35c6a211afec0d4d4e07f3a2410bd44bf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bilirubin - blood</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Humans</topic><topic>Hyperbilirubinemia, Neonatal - blood</topic><topic>Hyperbilirubinemia, Neonatal - therapy</topic><topic>Infant, Newborn</topic><topic>Isomerism</topic><topic>Light</topic><topic>Oxidoreductases Acting on CH-CH Group Donors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okada, Hitoshi</creatorcontrib><creatorcontrib>Kawada, Kou</creatorcontrib><creatorcontrib>Itoh, Susumu</creatorcontrib><creatorcontrib>Ozaki, Miyo</creatorcontrib><creatorcontrib>Kakutani, Isami</creatorcontrib><creatorcontrib>Arai, Takeshi</creatorcontrib><creatorcontrib>Koyano, Kosuke</creatorcontrib><creatorcontrib>Yasuda, Saneyuki</creatorcontrib><creatorcontrib>Iwase, Takashi</creatorcontrib><creatorcontrib>Murao, Koji</creatorcontrib><creatorcontrib>Kusaka, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okada, Hitoshi</au><au>Kawada, Kou</au><au>Itoh, Susumu</au><au>Ozaki, Miyo</au><au>Kakutani, Isami</au><au>Arai, Takeshi</au><au>Koyano, Kosuke</au><au>Yasuda, Saneyuki</au><au>Iwase, Takashi</au><au>Murao, Koji</au><au>Kusaka, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method</atitle><jtitle>Annals of clinical biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>55</volume><issue>2</issue><spage>276</spage><epage>280</epage><pages>276-280</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><abstract>Background
We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light.
Methods
Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24 h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography.
Results
Direct bilirubin increased significantly from 0.61 to 2.36 mg/dL. The respective ratios of bilirubin photoisomers before and after exposure were as follows: cyclobilirubin (0.007 to 0.29) and (EZ)-bilirubin (0.018 to 0.041) increased significantly, (ZZ)-bilirubin decreased 0.84 to 0.55 significantly. The difference of the cyclobilirubin concentration was most closely associated with those of the direct bilirubin concentration.
Conclusion
Direct bilirubin value was increased after exposure to the room light, and increase in direct bilirubin was significantly correlated by cyclobilirubin increase in the serum samples from neonates with indirect hyperbilirubinemia.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>28656812</pmid><doi>10.1177/0004563217716474</doi><tpages>5</tpages></addata></record> |
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| ispartof | Annals of clinical biochemistry, 2018-03, Vol.55 (2), p.276-280 |
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| subjects | Bilirubin - blood Chromatography, High Pressure Liquid Humans Hyperbilirubinemia, Neonatal - blood Hyperbilirubinemia, Neonatal - therapy Infant, Newborn Isomerism Light Oxidoreductases Acting on CH-CH Group Donors - metabolism |
| title | Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method |
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