A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms
We have exploited a compact and facile microfluidic droplet creation device consisting of a poly(dimethylsiloxane) microfluidic chip possessing T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir, and a piezoelectric (PZT) diaphragm micropump with controller. Air was evacuate...
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| Veröffentlicht in: | Electrophoresis 2017-10, Vol.38 (20), p.2666-2672 |
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| container_title | Electrophoresis |
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| creator | Okura, Naoaki Nakashoji, Yuta Koshirogane, Toshihiro Kondo, Masaki Tanaka, Yugo Inoue, Kohei Hashimoto, Masahiko |
| description | We have exploited a compact and facile microfluidic droplet creation device consisting of a poly(dimethylsiloxane) microfluidic chip possessing T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir, and a piezoelectric (PZT) diaphragm micropump with controller. Air was evacuated from the outlet reservoir using the PZT pump, reducing the pressure inside. The reduced pressure within the outlet reservoir pulled oil and aqueous solution preloaded in the inlet reservoirs into the microchannels, which then merged at the T‐junction, successfully forming water‐in‐oil emulsion droplets at a rate of ∼1000 per second with minimal sample loss. We confirmed that the onset of droplet formation occurred immediately after turning on the pump ( |
| doi_str_mv | 10.1002/elps.201700039 |
| format | Article |
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Air was evacuated from the outlet reservoir using the PZT pump, reducing the pressure inside. The reduced pressure within the outlet reservoir pulled oil and aqueous solution preloaded in the inlet reservoirs into the microchannels, which then merged at the T‐junction, successfully forming water‐in‐oil emulsion droplets at a rate of ∼1000 per second with minimal sample loss. We confirmed that the onset of droplet formation occurred immediately after turning on the pump (<1 s). Over repeated runs, droplet formation was highly reproducible, with droplet size purity (polydispersity, <4%) comparable to that achieved using other microfluidic droplet preparation techniques. We also demonstrated single‐molecule PCR amplification in the created droplets, suggesting that the device could be used for effective droplet digital PCR platforms in most laboratories without requiring great expense, space, or time for acquiring technical skills.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.201700039</identifier><identifier>PMID: 28657130</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Diaphragms ; Dimethylpolysiloxanes - chemistry ; Droplet digital PCR ; Droplets ; Emulsion ; Emulsions ; Equipment Design ; Infusion Pumps ; Lab-On-A-Chip Devices ; Lead zirconate titanates ; Microchannels ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Microfluidic device ; Micropumps ; Particle Size ; Piezoelectric diaphragm micropump ; Piezoelectricity ; Platforms ; Poly(dimethylsiloxane) ; Polydimethylsiloxane ; Polydispersity ; Polymerase Chain Reaction - instrumentation ; Polymerase Chain Reaction - methods ; Reservoirs ; Water</subject><ispartof>Electrophoresis, 2017-10, Vol.38 (20), p.2666-2672</ispartof><rights>2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4715-b586cb51fdc30f1ae8e2accb74e21e87ca6ef46b7d0f824bb12537cbc077effd3</citedby><cites>FETCH-LOGICAL-c4715-b586cb51fdc30f1ae8e2accb74e21e87ca6ef46b7d0f824bb12537cbc077effd3</cites><orcidid>0000-0002-1355-8738</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.201700039$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.201700039$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28657130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okura, Naoaki</creatorcontrib><creatorcontrib>Nakashoji, Yuta</creatorcontrib><creatorcontrib>Koshirogane, Toshihiro</creatorcontrib><creatorcontrib>Kondo, Masaki</creatorcontrib><creatorcontrib>Tanaka, Yugo</creatorcontrib><creatorcontrib>Inoue, Kohei</creatorcontrib><creatorcontrib>Hashimoto, Masahiko</creatorcontrib><title>A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms</title><title>Electrophoresis</title><addtitle>Electrophoresis</addtitle><description>We have exploited a compact and facile microfluidic droplet creation device consisting of a poly(dimethylsiloxane) microfluidic chip possessing T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir, and a piezoelectric (PZT) diaphragm micropump with controller. Air was evacuated from the outlet reservoir using the PZT pump, reducing the pressure inside. The reduced pressure within the outlet reservoir pulled oil and aqueous solution preloaded in the inlet reservoirs into the microchannels, which then merged at the T‐junction, successfully forming water‐in‐oil emulsion droplets at a rate of ∼1000 per second with minimal sample loss. We confirmed that the onset of droplet formation occurred immediately after turning on the pump (<1 s). Over repeated runs, droplet formation was highly reproducible, with droplet size purity (polydispersity, <4%) comparable to that achieved using other microfluidic droplet preparation techniques. We also demonstrated single‐molecule PCR amplification in the created droplets, suggesting that the device could be used for effective droplet digital PCR platforms in most laboratories without requiring great expense, space, or time for acquiring technical skills.</description><subject>Diaphragms</subject><subject>Dimethylpolysiloxanes - chemistry</subject><subject>Droplet digital PCR</subject><subject>Droplets</subject><subject>Emulsion</subject><subject>Emulsions</subject><subject>Equipment Design</subject><subject>Infusion Pumps</subject><subject>Lab-On-A-Chip Devices</subject><subject>Lead zirconate titanates</subject><subject>Microchannels</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidic device</subject><subject>Micropumps</subject><subject>Particle Size</subject><subject>Piezoelectric diaphragm micropump</subject><subject>Piezoelectricity</subject><subject>Platforms</subject><subject>Poly(dimethylsiloxane)</subject><subject>Polydimethylsiloxane</subject><subject>Polydispersity</subject><subject>Polymerase Chain Reaction - instrumentation</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reservoirs</subject><subject>Water</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEYQINY7Fq9epSAl15mzc9J9liWWoUFS9XzkEm-rCmZmZjMVOrd_9ssW_fgxVPgy8vjCw-hN5SsKSHsPcRU1oxQRQjhm2doRSVjDWs1f45Wdcwbork8Ry9Lua-I2AjxAp0z3UpFOVmh31fYTkMydsZmdNgbGyLgIdg8-bgEFyx2eUoRZmwzmDlMI3bwECzgpYRxjw1OAX5NEMHO-UAHk75nsx-OkrQMCfspnywu7MNsIr7d3uEUzVzvhvIKnXkTC7x-Oi_Qtw_XX7cfm93nm0_bq11jhaKy6aVubS-pd5YTTw1oYMbaXglgFLSypgUv2l454jUTfU-Z5Mr2ligF3jt-gS6P3pSnHwuUuRtCsRCjGWFaSkc3VEhNRSsq-u4f9H5a8li3q5QklAqhD9T6SNWvlpLBdymHweTHjpLuEKg7BOpOgeqDt0_apR_AnfC_RSogjsDPGuLxP7ruenf7pWVc8j_7xp8C</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Okura, Naoaki</creator><creator>Nakashoji, Yuta</creator><creator>Koshirogane, Toshihiro</creator><creator>Kondo, Masaki</creator><creator>Tanaka, Yugo</creator><creator>Inoue, Kohei</creator><creator>Hashimoto, Masahiko</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1355-8738</orcidid></search><sort><creationdate>201710</creationdate><title>A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms</title><author>Okura, Naoaki ; Nakashoji, Yuta ; Koshirogane, Toshihiro ; Kondo, Masaki ; Tanaka, Yugo ; Inoue, Kohei ; Hashimoto, Masahiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4715-b586cb51fdc30f1ae8e2accb74e21e87ca6ef46b7d0f824bb12537cbc077effd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Diaphragms</topic><topic>Dimethylpolysiloxanes - chemistry</topic><topic>Droplet digital PCR</topic><topic>Droplets</topic><topic>Emulsion</topic><topic>Emulsions</topic><topic>Equipment Design</topic><topic>Infusion Pumps</topic><topic>Lab-On-A-Chip Devices</topic><topic>Lead zirconate titanates</topic><topic>Microchannels</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidic device</topic><topic>Micropumps</topic><topic>Particle Size</topic><topic>Piezoelectric diaphragm micropump</topic><topic>Piezoelectricity</topic><topic>Platforms</topic><topic>Poly(dimethylsiloxane)</topic><topic>Polydimethylsiloxane</topic><topic>Polydispersity</topic><topic>Polymerase Chain Reaction - instrumentation</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reservoirs</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okura, Naoaki</creatorcontrib><creatorcontrib>Nakashoji, Yuta</creatorcontrib><creatorcontrib>Koshirogane, Toshihiro</creatorcontrib><creatorcontrib>Kondo, Masaki</creatorcontrib><creatorcontrib>Tanaka, Yugo</creatorcontrib><creatorcontrib>Inoue, Kohei</creatorcontrib><creatorcontrib>Hashimoto, Masahiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okura, Naoaki</au><au>Nakashoji, Yuta</au><au>Koshirogane, Toshihiro</au><au>Kondo, Masaki</au><au>Tanaka, Yugo</au><au>Inoue, Kohei</au><au>Hashimoto, Masahiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms</atitle><jtitle>Electrophoresis</jtitle><addtitle>Electrophoresis</addtitle><date>2017-10</date><risdate>2017</risdate><volume>38</volume><issue>20</issue><spage>2666</spage><epage>2672</epage><pages>2666-2672</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>We have exploited a compact and facile microfluidic droplet creation device consisting of a poly(dimethylsiloxane) microfluidic chip possessing T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir, and a piezoelectric (PZT) diaphragm micropump with controller. Air was evacuated from the outlet reservoir using the PZT pump, reducing the pressure inside. The reduced pressure within the outlet reservoir pulled oil and aqueous solution preloaded in the inlet reservoirs into the microchannels, which then merged at the T‐junction, successfully forming water‐in‐oil emulsion droplets at a rate of ∼1000 per second with minimal sample loss. We confirmed that the onset of droplet formation occurred immediately after turning on the pump (<1 s). Over repeated runs, droplet formation was highly reproducible, with droplet size purity (polydispersity, <4%) comparable to that achieved using other microfluidic droplet preparation techniques. We also demonstrated single‐molecule PCR amplification in the created droplets, suggesting that the device could be used for effective droplet digital PCR platforms in most laboratories without requiring great expense, space, or time for acquiring technical skills.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28657130</pmid><doi>10.1002/elps.201700039</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1355-8738</orcidid></addata></record> |
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| subjects | Diaphragms Dimethylpolysiloxanes - chemistry Droplet digital PCR Droplets Emulsion Emulsions Equipment Design Infusion Pumps Lab-On-A-Chip Devices Lead zirconate titanates Microchannels Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidic device Micropumps Particle Size Piezoelectric diaphragm micropump Piezoelectricity Platforms Poly(dimethylsiloxane) Polydimethylsiloxane Polydispersity Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Reservoirs Water |
| title | A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms |
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