ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding
The cytogenetic characteristics of Eigenmannia aff. trilineata were analyzed by basic and molecular cytogenetics, applying fluorescent in situ hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (...
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| Veröffentlicht in: | Zebrafish 2017-10, Vol.14 (5), p.464-470 |
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| creator | Araya-Jaime, Cristian Mateussi, Nadayca T. Bonani Utsunomia, Ricardo Costa-Silva, Guilherme J. Oliveira, Claudio Foresti, Fausto |
| description | The cytogenetic characteristics of
Eigenmannia
aff.
trilineata
were analyzed by basic and molecular cytogenetics, applying fluorescent
in situ
hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11. A site for rDNA 5S was synthetic with a cluster of rDNA 18S near the centromere on the long arm of only one homologue of pair 11. Other clusters for 5S rDNA were sited on pairs 7, 10, 12, 13, and 16. Further, 5S rDNA was co-located with U2 cluster in the pericentromeric region of pair 12. Joint analysis of DNA barcoding from cytochrome c oxidase subunit I (COI) sequences, generated from the karyotyped samples of
E.
aff.
trilineata
, and sequences of other Gymnotiforms recognized
E
. aff.
trilineata
as an Operational Taxonomic Unit. Results foreground the hypothesis that cytotypes are independent evolution units as cryptic species with a low morphological differentiation level, although with high genetic/karyotype differentiation rates. |
| doi_str_mv | 10.1089/zeb.2017.1422 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1914581032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1914581032</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-a51a37472766bf71f1588f5ee24e665d1b2a088b7a1eaa7db6b7583eaf1a00923</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhiMEoqVw5IoscSmHbO3YjpO9laUUpFIOu1z2Ek2S8dZVYi-2V5C-Ei-Joy0cuHCa0eibf0b6suw1owtGq_riAdtFQZlaMFEUT7JTJqXKKymKp3Mv5Nyrk-xFCPeUcl5z8Tw7KapSCi7ZafZru73Y0iXZ3CG5xR9kPYWII3GarPEnWd15N7rgRgzEWHJldmhHsNYAAa0XJHozGIsQgZxvcECXls2SXE-jddFo59Pikqwjeuv20870gO9SKHjo0sw8YE_aiXxxA3aHATxZTdGlExhNFwjYnny4vSTvwXeuN3b3MnumYQj46rGeZd8-Xm1Wn_Kbr9efV5c3ecdLGXOQDLgSqlBl2WrFNJNVpSViIbAsZc_aAmhVtQoYAqi-LVslK46gGVBaF_wsOz_m7r37fsAQm9GEDocBLLpDaFjNhKwY5TP69h_03h28Td8lSigmWF2rROVHqvMuBI-62Xszgp8aRpvZYpMsNrPFZraY-DePqYd2xP4v_UdbAvgRmMfJx2CwRR__E_sbgQCqSw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1947141997</pqid></control><display><type>article</type><title>ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Araya-Jaime, Cristian ; Mateussi, Nadayca T. Bonani ; Utsunomia, Ricardo ; Costa-Silva, Guilherme J. ; Oliveira, Claudio ; Foresti, Fausto</creator><creatorcontrib>Araya-Jaime, Cristian ; Mateussi, Nadayca T. Bonani ; Utsunomia, Ricardo ; Costa-Silva, Guilherme J. ; Oliveira, Claudio ; Foresti, Fausto</creatorcontrib><description>The cytogenetic characteristics of
Eigenmannia
aff.
trilineata
were analyzed by basic and molecular cytogenetics, applying fluorescent
in situ
hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11. A site for rDNA 5S was synthetic with a cluster of rDNA 18S near the centromere on the long arm of only one homologue of pair 11. Other clusters for 5S rDNA were sited on pairs 7, 10, 12, 13, and 16. Further, 5S rDNA was co-located with U2 cluster in the pericentromeric region of pair 12. Joint analysis of DNA barcoding from cytochrome c oxidase subunit I (COI) sequences, generated from the karyotyped samples of
E.
aff.
trilineata
, and sequences of other Gymnotiforms recognized
E
. aff.
trilineata
as an Operational Taxonomic Unit. Results foreground the hypothesis that cytotypes are independent evolution units as cryptic species with a low morphological differentiation level, although with high genetic/karyotype differentiation rates.</description><identifier>ISSN: 1545-8547</identifier><identifier>EISSN: 1557-8542</identifier><identifier>DOI: 10.1089/zeb.2017.1422</identifier><identifier>PMID: 28654351</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Banding ; Biological evolution ; Chromosome Mapping ; Chromosomes ; Clusters ; Cryptic species ; Cytochrome ; Cytochrome-c oxidase ; Cytogenetics ; Deoxyribonucleic acid ; Differentiation ; DNA ; DNA barcoding ; DNA Barcoding, Taxonomic - methods ; DNA probes ; Eigenmannia ; Eigenmannia trilineata ; EvoFish ; Females ; Fluorescence ; Fluorescence in situ hybridization ; Gene sequencing ; Gymnotiformes ; Gymnotiformes - classification ; Gymnotiformes - genetics ; Heterochromatin ; Homology ; Karyotypes ; Karyotyping ; Males ; Nucleoli ; Nucleotide sequence ; Oxidase ; Polymorphism ; Probes ; Repetitive Sequences, Nucleic Acid ; RNA ; Sex ; Sex Chromosomes ; snRNA ; Sternopygidae ; Teleostei</subject><ispartof>Zebrafish, 2017-10, Vol.14 (5), p.464-470</ispartof><rights>2017, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2017, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-a51a37472766bf71f1588f5ee24e665d1b2a088b7a1eaa7db6b7583eaf1a00923</citedby><cites>FETCH-LOGICAL-c365t-a51a37472766bf71f1588f5ee24e665d1b2a088b7a1eaa7db6b7583eaf1a00923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28654351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araya-Jaime, Cristian</creatorcontrib><creatorcontrib>Mateussi, Nadayca T. Bonani</creatorcontrib><creatorcontrib>Utsunomia, Ricardo</creatorcontrib><creatorcontrib>Costa-Silva, Guilherme J.</creatorcontrib><creatorcontrib>Oliveira, Claudio</creatorcontrib><creatorcontrib>Foresti, Fausto</creatorcontrib><title>ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding</title><title>Zebrafish</title><addtitle>Zebrafish</addtitle><description>The cytogenetic characteristics of
Eigenmannia
aff.
trilineata
were analyzed by basic and molecular cytogenetics, applying fluorescent
in situ
hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11. A site for rDNA 5S was synthetic with a cluster of rDNA 18S near the centromere on the long arm of only one homologue of pair 11. Other clusters for 5S rDNA were sited on pairs 7, 10, 12, 13, and 16. Further, 5S rDNA was co-located with U2 cluster in the pericentromeric region of pair 12. Joint analysis of DNA barcoding from cytochrome c oxidase subunit I (COI) sequences, generated from the karyotyped samples of
E.
aff.
trilineata
, and sequences of other Gymnotiforms recognized
E
. aff.
trilineata
as an Operational Taxonomic Unit. Results foreground the hypothesis that cytotypes are independent evolution units as cryptic species with a low morphological differentiation level, although with high genetic/karyotype differentiation rates.</description><subject>Animals</subject><subject>Banding</subject><subject>Biological evolution</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Clusters</subject><subject>Cryptic species</subject><subject>Cytochrome</subject><subject>Cytochrome-c oxidase</subject><subject>Cytogenetics</subject><subject>Deoxyribonucleic acid</subject><subject>Differentiation</subject><subject>DNA</subject><subject>DNA barcoding</subject><subject>DNA Barcoding, Taxonomic - methods</subject><subject>DNA probes</subject><subject>Eigenmannia</subject><subject>Eigenmannia trilineata</subject><subject>EvoFish</subject><subject>Females</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Gene sequencing</subject><subject>Gymnotiformes</subject><subject>Gymnotiformes - classification</subject><subject>Gymnotiformes - genetics</subject><subject>Heterochromatin</subject><subject>Homology</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Males</subject><subject>Nucleoli</subject><subject>Nucleotide sequence</subject><subject>Oxidase</subject><subject>Polymorphism</subject><subject>Probes</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA</subject><subject>Sex</subject><subject>Sex Chromosomes</subject><subject>snRNA</subject><subject>Sternopygidae</subject><subject>Teleostei</subject><issn>1545-8547</issn><issn>1557-8542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEoqVw5IoscSmHbO3YjpO9laUUpFIOu1z2Ek2S8dZVYi-2V5C-Ei-Joy0cuHCa0eibf0b6suw1owtGq_riAdtFQZlaMFEUT7JTJqXKKymKp3Mv5Nyrk-xFCPeUcl5z8Tw7KapSCi7ZafZru73Y0iXZ3CG5xR9kPYWII3GarPEnWd15N7rgRgzEWHJldmhHsNYAAa0XJHozGIsQgZxvcECXls2SXE-jddFo59Pikqwjeuv20870gO9SKHjo0sw8YE_aiXxxA3aHATxZTdGlExhNFwjYnny4vSTvwXeuN3b3MnumYQj46rGeZd8-Xm1Wn_Kbr9efV5c3ecdLGXOQDLgSqlBl2WrFNJNVpSViIbAsZc_aAmhVtQoYAqi-LVslK46gGVBaF_wsOz_m7r37fsAQm9GEDocBLLpDaFjNhKwY5TP69h_03h28Td8lSigmWF2rROVHqvMuBI-62Xszgp8aRpvZYpMsNrPFZraY-DePqYd2xP4v_UdbAvgRmMfJx2CwRR__E_sbgQCqSw</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Araya-Jaime, Cristian</creator><creator>Mateussi, Nadayca T. Bonani</creator><creator>Utsunomia, Ricardo</creator><creator>Costa-Silva, Guilherme J.</creator><creator>Oliveira, Claudio</creator><creator>Foresti, Fausto</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171001</creationdate><title>ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding</title><author>Araya-Jaime, Cristian ; Mateussi, Nadayca T. 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Bonani</creatorcontrib><creatorcontrib>Utsunomia, Ricardo</creatorcontrib><creatorcontrib>Costa-Silva, Guilherme J.</creatorcontrib><creatorcontrib>Oliveira, Claudio</creatorcontrib><creatorcontrib>Foresti, Fausto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Zebrafish</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araya-Jaime, Cristian</au><au>Mateussi, Nadayca T. Bonani</au><au>Utsunomia, Ricardo</au><au>Costa-Silva, Guilherme J.</au><au>Oliveira, Claudio</au><au>Foresti, Fausto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding</atitle><jtitle>Zebrafish</jtitle><addtitle>Zebrafish</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>14</volume><issue>5</issue><spage>464</spage><epage>470</epage><pages>464-470</pages><issn>1545-8547</issn><eissn>1557-8542</eissn><abstract>The cytogenetic characteristics of
Eigenmannia
aff.
trilineata
were analyzed by basic and molecular cytogenetics, applying fluorescent
in situ
hybridization, with 18S and 5S rDNA and U2 snRNA probes. The species revealed a kind of polymorphism associated to ZZ/Z0 type sex chromosomes, with 2n = 32 (8m+2sm+22a, NF = 42) in all males under analysis, whereas females evidenced 2n = 31 (8m+1sm+22a, NF = 40). C-banding showed constitutive heterochromatin restricted to the pericentromeric region of all chromosomes and single-nucleolus organized regions on pair 11. A site for rDNA 5S was synthetic with a cluster of rDNA 18S near the centromere on the long arm of only one homologue of pair 11. Other clusters for 5S rDNA were sited on pairs 7, 10, 12, 13, and 16. Further, 5S rDNA was co-located with U2 cluster in the pericentromeric region of pair 12. Joint analysis of DNA barcoding from cytochrome c oxidase subunit I (COI) sequences, generated from the karyotyped samples of
E.
aff.
trilineata
, and sequences of other Gymnotiforms recognized
E
. aff.
trilineata
as an Operational Taxonomic Unit. Results foreground the hypothesis that cytotypes are independent evolution units as cryptic species with a low morphological differentiation level, although with high genetic/karyotype differentiation rates.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>28654351</pmid><doi>10.1089/zeb.2017.1422</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Zebrafish, 2017-10, Vol.14 (5), p.464-470 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_1914581032 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Banding Biological evolution Chromosome Mapping Chromosomes Clusters Cryptic species Cytochrome Cytochrome-c oxidase Cytogenetics Deoxyribonucleic acid Differentiation DNA DNA barcoding DNA Barcoding, Taxonomic - methods DNA probes Eigenmannia Eigenmannia trilineata EvoFish Females Fluorescence Fluorescence in situ hybridization Gene sequencing Gymnotiformes Gymnotiformes - classification Gymnotiformes - genetics Heterochromatin Homology Karyotypes Karyotyping Males Nucleoli Nucleotide sequence Oxidase Polymorphism Probes Repetitive Sequences, Nucleic Acid RNA Sex Sex Chromosomes snRNA Sternopygidae Teleostei |
| title | ZZ/Z0: The New System of Sex Chromosomes in Eigenmannia aff. trilineata (Teleostei: Gymnotiformes: Sternopygidae) Characterized by Molecular Cytogenetics and DNA Barcoding |
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