Mononuclear cells of umbilical cord blood differentiation to granulocyte cell in vitro
To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells. Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chose...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2017-06, Vol.38 (6), p.532 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Chen, L Xie, X Y Nie, J Q Chen, D L Huang, A P Fang, F Qu, M Y Nan, X He, L J Fan, Z Yue, W Pei, X T |
| description | To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells.
Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well.
Compared to fetal bovine serum (FBS) treatment group, cell viability, counts and differentiation rate of granulocytes induced by X-VIVO(TM) 15 combined with TPO, SCF, G-CSF but without FBS were superior. And X-VIVO(TM)15 medium was better than SCGM medium at effectiveness and cost. Using two-stage mode of hematopoietic stem cell expansion followed by granulocyte induction with X-VIVO(TM)15 combining TPO, SCF and G-CSF, cell proliferation was nearly 132 times at day 21. Flow c |
| doi_str_mv | 10.3760/cma.j.issn.0253-2727.2017.06.013 |
| format | Article |
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Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well.
Compared to fetal bovine serum (FBS) treatment group, cell viability, counts and differentiation rate of granulocytes induced by X-VIVO(TM) 15 combined with TPO, SCF, G-CSF but without FBS were superior. And X-VIVO(TM)15 medium was better than SCGM medium at effectiveness and cost. Using two-stage mode of hematopoietic stem cell expansion followed by granulocyte induction with X-VIVO(TM)15 combining TPO, SCF and G-CSF, cell proliferation was nearly 132 times at day 21. Flow c</description><identifier>ISSN: 0253-2727</identifier><identifier>DOI: 10.3760/cma.j.issn.0253-2727.2017.06.013</identifier><identifier>PMID: 28655099</identifier><language>chi</language><publisher>China</publisher><subject>Cell Differentiation ; Cells, Cultured ; Fetal Blood ; Flow Cytometry ; Granulocyte Colony-Stimulating Factor ; Granulocytes ; Hematopoiesis ; Hematopoietic Stem Cells ; Humans ; Neutrophils ; Umbilical Cord</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2017-06, Vol.38 (6), p.532</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28655099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Xie, X Y</creatorcontrib><creatorcontrib>Nie, J Q</creatorcontrib><creatorcontrib>Chen, D L</creatorcontrib><creatorcontrib>Huang, A P</creatorcontrib><creatorcontrib>Fang, F</creatorcontrib><creatorcontrib>Qu, M Y</creatorcontrib><creatorcontrib>Nan, X</creatorcontrib><creatorcontrib>He, L J</creatorcontrib><creatorcontrib>Fan, Z</creatorcontrib><creatorcontrib>Yue, W</creatorcontrib><creatorcontrib>Pei, X T</creatorcontrib><title>Mononuclear cells of umbilical cord blood differentiation to granulocyte cell in vitro</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells.
Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well.
Compared to fetal bovine serum (FBS) treatment group, cell viability, counts and differentiation rate of granulocytes induced by X-VIVO(TM) 15 combined with TPO, SCF, G-CSF but without FBS were superior. And X-VIVO(TM)15 medium was better than SCGM medium at effectiveness and cost. Using two-stage mode of hematopoietic stem cell expansion followed by granulocyte induction with X-VIVO(TM)15 combining TPO, SCF and G-CSF, cell proliferation was nearly 132 times at day 21. Flow c</description><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Fetal Blood</subject><subject>Flow Cytometry</subject><subject>Granulocyte Colony-Stimulating Factor</subject><subject>Granulocytes</subject><subject>Hematopoiesis</subject><subject>Hematopoietic Stem Cells</subject><subject>Humans</subject><subject>Neutrophils</subject><subject>Umbilical Cord</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UMtOAyEA5KCxTe0vGI697MpjgXI0jY8mNV7U6wZYMBgWKrAm_XurVk-TTGYmMwPACqOWCo6uzaja99aXEltEGG2IIKIlCIsW8RZhegbm__wMLEvxGjFM-ZpSdAFmZM0ZQ1LOwetjiilOJliVobEhFJgcnEbtgzcqQJPyAHVIaYCDd85mG6tX1acIa4JvWcUpJHOo9scMfYSfvuZ0Cc6dCsUuT7gAL3e3z5uHZvd0v93c7Jo9Jrw2jlGhOsk7xDuhhGVMWMUNpsclg3QaM4IZdlI4ISTTmtjBGW0HbDmWUiK6AKvf3H1OH5MttR99-W6iok1T6bHEHVtjRLqj9OoknfRoh36f_ajyof_7gn4BrhBk5A</recordid><startdate>20170614</startdate><enddate>20170614</enddate><creator>Chen, L</creator><creator>Xie, X Y</creator><creator>Nie, J Q</creator><creator>Chen, D L</creator><creator>Huang, A P</creator><creator>Fang, F</creator><creator>Qu, M Y</creator><creator>Nan, X</creator><creator>He, L J</creator><creator>Fan, Z</creator><creator>Yue, W</creator><creator>Pei, X T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20170614</creationdate><title>Mononuclear cells of umbilical cord blood differentiation to granulocyte cell in vitro</title><author>Chen, L ; Xie, X Y ; Nie, J Q ; Chen, D L ; Huang, A P ; Fang, F ; Qu, M Y ; Nan, X ; He, L J ; Fan, Z ; Yue, W ; Pei, X T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-f537a49640647a7e557ea6c13017d9fb152151f97f7795bb2edfcbed1e6199903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2017</creationdate><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Fetal Blood</topic><topic>Flow Cytometry</topic><topic>Granulocyte Colony-Stimulating Factor</topic><topic>Granulocytes</topic><topic>Hematopoiesis</topic><topic>Hematopoietic Stem Cells</topic><topic>Humans</topic><topic>Neutrophils</topic><topic>Umbilical Cord</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Xie, X Y</creatorcontrib><creatorcontrib>Nie, J Q</creatorcontrib><creatorcontrib>Chen, D L</creatorcontrib><creatorcontrib>Huang, A P</creatorcontrib><creatorcontrib>Fang, F</creatorcontrib><creatorcontrib>Qu, M Y</creatorcontrib><creatorcontrib>Nan, X</creatorcontrib><creatorcontrib>He, L J</creatorcontrib><creatorcontrib>Fan, Z</creatorcontrib><creatorcontrib>Yue, W</creatorcontrib><creatorcontrib>Pei, X T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, L</au><au>Xie, X Y</au><au>Nie, J Q</au><au>Chen, D L</au><au>Huang, A P</au><au>Fang, F</au><au>Qu, M Y</au><au>Nan, X</au><au>He, L J</au><au>Fan, Z</au><au>Yue, W</au><au>Pei, X T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mononuclear cells of umbilical cord blood differentiation to granulocyte cell in vitro</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2017-06-14</date><risdate>2017</risdate><volume>38</volume><issue>6</issue><spage>532</spage><pages>532-</pages><issn>0253-2727</issn><abstract>To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells.
Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well.
Compared to fetal bovine serum (FBS) treatment group, cell viability, counts and differentiation rate of granulocytes induced by X-VIVO(TM) 15 combined with TPO, SCF, G-CSF but without FBS were superior. And X-VIVO(TM)15 medium was better than SCGM medium at effectiveness and cost. Using two-stage mode of hematopoietic stem cell expansion followed by granulocyte induction with X-VIVO(TM)15 combining TPO, SCF and G-CSF, cell proliferation was nearly 132 times at day 21. Flow c</abstract><cop>China</cop><pmid>28655099</pmid><doi>10.3760/cma.j.issn.0253-2727.2017.06.013</doi></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2017-06, Vol.38 (6), p.532 |
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| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cell Differentiation Cells, Cultured Fetal Blood Flow Cytometry Granulocyte Colony-Stimulating Factor Granulocytes Hematopoiesis Hematopoietic Stem Cells Humans Neutrophils Umbilical Cord |
| title | Mononuclear cells of umbilical cord blood differentiation to granulocyte cell in vitro |
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