A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip
[Display omitted] •A quantitative carbapenemase assay was developed using LDI-MS with a parylene-matrix chip.•Reproducibility (spot-to-spot and shot-to-shot) of LDI-MS was presented with carbapenems.•A carbapenem-susceptibility test was carried out using 51 bacterial strains.•LDI-MS presented more s...
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| Veröffentlicht in: | Enzyme and microbial technology 2017-09, Vol.104, p.56-68 |
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| container_title | Enzyme and microbial technology |
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| creator | Park, Jong-Min Kim, Jo-Il Noh, Joo-Yoon Kim, Mira Kang, Min-Jung Pyun, Jae-Chul |
| description | [Display omitted]
•A quantitative carbapenemase assay was developed using LDI-MS with a parylene-matrix chip.•Reproducibility (spot-to-spot and shot-to-shot) of LDI-MS was presented with carbapenems.•A carbapenem-susceptibility test was carried out using 51 bacterial strains.•LDI-MS presented more sensitive and selective results than conventional MALDI-TOF MS.
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix. |
| doi_str_mv | 10.1016/j.enzmictec.2017.05.010 |
| format | Article |
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•A quantitative carbapenemase assay was developed using LDI-MS with a parylene-matrix chip.•Reproducibility (spot-to-spot and shot-to-shot) of LDI-MS was presented with carbapenems.•A carbapenem-susceptibility test was carried out using 51 bacterial strains.•LDI-MS presented more sensitive and selective results than conventional MALDI-TOF MS.
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/j.enzmictec.2017.05.010</identifier><identifier>PMID: 28648181</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibiotic-susceptibility test ; Bacteria - drug effects ; Bacteria - enzymology ; Bacterial Proteins - analysis ; beta-Lactam Resistance ; beta-Lactamases - analysis ; Carbapenem ; Carbapenems - metabolism ; Carbapenems - pharmacology ; Gentisates ; Laser desorption/ionization mass spectrometry (LDI–MS) ; MALDI-TOF MS ; Microbial Sensitivity Tests ; Parylene-matrix chip ; Polymers ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - statistics & numerical data ; Xylenes</subject><ispartof>Enzyme and microbial technology, 2017-09, Vol.104, p.56-68</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-7d52771afa7a81d01c910d7f214790f36bb94035680c41a7151bf03dabbaf5c33</citedby><cites>FETCH-LOGICAL-c408t-7d52771afa7a81d01c910d7f214790f36bb94035680c41a7151bf03dabbaf5c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.enzmictec.2017.05.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28648181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Jong-Min</creatorcontrib><creatorcontrib>Kim, Jo-Il</creatorcontrib><creatorcontrib>Noh, Joo-Yoon</creatorcontrib><creatorcontrib>Kim, Mira</creatorcontrib><creatorcontrib>Kang, Min-Jung</creatorcontrib><creatorcontrib>Pyun, Jae-Chul</creatorcontrib><title>A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip</title><title>Enzyme and microbial technology</title><addtitle>Enzyme Microb Technol</addtitle><description>[Display omitted]
•A quantitative carbapenemase assay was developed using LDI-MS with a parylene-matrix chip.•Reproducibility (spot-to-spot and shot-to-shot) of LDI-MS was presented with carbapenems.•A carbapenem-susceptibility test was carried out using 51 bacterial strains.•LDI-MS presented more sensitive and selective results than conventional MALDI-TOF MS.
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.</description><subject>Antibiotic-susceptibility test</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - enzymology</subject><subject>Bacterial Proteins - analysis</subject><subject>beta-Lactam Resistance</subject><subject>beta-Lactamases - analysis</subject><subject>Carbapenem</subject><subject>Carbapenems - metabolism</subject><subject>Carbapenems - pharmacology</subject><subject>Gentisates</subject><subject>Laser desorption/ionization mass spectrometry (LDI–MS)</subject><subject>MALDI-TOF MS</subject><subject>Microbial Sensitivity Tests</subject><subject>Parylene-matrix chip</subject><subject>Polymers</subject><subject>Reproducibility of Results</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - statistics & numerical data</subject><subject>Xylenes</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv2zAMgIViQ5t2_QudjrvYJf2IrGNQrNuAArtsZ0GW6EaBXxOdYsmvn4J0vfZAiKA-ktInxGeEHAHX97ucxuMQ3EIuLwBVDnUOCBdihY3SGWjQH8QKsMIMikJfiWvmHUAqVHApropmXTXY4EocN3Ibnrf9QTKNHJbwQtLZ2NqZRhosk7TM9iD3HMZn2adClJ54ivMSpvE-RTjaUyoTzJJnckucBlriQbaJ9jJdWTnbeOjTxGywSwx_pduG-ZP42Nme6fb1vBG_H7_-eviePf389uNh85S5CpolU74ulELbWWUb9IBOI3jVFVgpDV25bltdQVmvG3AVWoU1th2U3rat7WpXljfiy3nuHKc_e-LFDIEd9b0dadqzQY1lU5aIOqHqjLo4MUfqzBzDkN5uEMxJvNmZN_HmJN5AbZL41Hn3umTfDuTf-v6bTsDmDFD66kugaNgFGh35EJMz46fw7pJ_7x2bfQ</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Park, Jong-Min</creator><creator>Kim, Jo-Il</creator><creator>Noh, Joo-Yoon</creator><creator>Kim, Mira</creator><creator>Kang, Min-Jung</creator><creator>Pyun, Jae-Chul</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170901</creationdate><title>A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip</title><author>Park, Jong-Min ; Kim, Jo-Il ; Noh, Joo-Yoon ; Kim, Mira ; Kang, Min-Jung ; Pyun, Jae-Chul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-7d52771afa7a81d01c910d7f214790f36bb94035680c41a7151bf03dabbaf5c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antibiotic-susceptibility test</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - enzymology</topic><topic>Bacterial Proteins - analysis</topic><topic>beta-Lactam Resistance</topic><topic>beta-Lactamases - analysis</topic><topic>Carbapenem</topic><topic>Carbapenems - metabolism</topic><topic>Carbapenems - pharmacology</topic><topic>Gentisates</topic><topic>Laser desorption/ionization mass spectrometry (LDI–MS)</topic><topic>MALDI-TOF MS</topic><topic>Microbial Sensitivity Tests</topic><topic>Parylene-matrix chip</topic><topic>Polymers</topic><topic>Reproducibility of Results</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - statistics & numerical data</topic><topic>Xylenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Jong-Min</creatorcontrib><creatorcontrib>Kim, Jo-Il</creatorcontrib><creatorcontrib>Noh, Joo-Yoon</creatorcontrib><creatorcontrib>Kim, Mira</creatorcontrib><creatorcontrib>Kang, Min-Jung</creatorcontrib><creatorcontrib>Pyun, Jae-Chul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Jong-Min</au><au>Kim, Jo-Il</au><au>Noh, Joo-Yoon</au><au>Kim, Mira</au><au>Kang, Min-Jung</au><au>Pyun, Jae-Chul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip</atitle><jtitle>Enzyme and microbial technology</jtitle><addtitle>Enzyme Microb Technol</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>104</volume><spage>56</spage><epage>68</epage><pages>56-68</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><abstract>[Display omitted]
•A quantitative carbapenemase assay was developed using LDI-MS with a parylene-matrix chip.•Reproducibility (spot-to-spot and shot-to-shot) of LDI-MS was presented with carbapenems.•A carbapenem-susceptibility test was carried out using 51 bacterial strains.•LDI-MS presented more sensitive and selective results than conventional MALDI-TOF MS.
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28648181</pmid><doi>10.1016/j.enzmictec.2017.05.010</doi><tpages>13</tpages></addata></record> |
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| subjects | Antibiotic-susceptibility test Bacteria - drug effects Bacteria - enzymology Bacterial Proteins - analysis beta-Lactam Resistance beta-Lactamases - analysis Carbapenem Carbapenems - metabolism Carbapenems - pharmacology Gentisates Laser desorption/ionization mass spectrometry (LDI–MS) MALDI-TOF MS Microbial Sensitivity Tests Parylene-matrix chip Polymers Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - statistics & numerical data Xylenes |
| title | A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip |
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