Influence of knockout of At4g20990 gene encoding α-CA4 on photosystem II light-harvesting antenna in plants grown under different light intensities and day lengths

Effect of knockout of the At4g20990 gene encoding α-carbonic anhydrase 4 (α-CA4) in Arabidopsis thaliana in plants grown in low light (LL, 80 μmol photons m −2  s −1 ) or in high light (HL, 400 μmol photons m −2  s −1 ) under long (LD, 16 h) or short (SD, 8 h) day length was studied. In α-CA4 knocko...

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Veröffentlicht in:Protoplasma 2018, Vol.255 (1), p.69-78
Hauptverfasser: Rudenko, Natalia N., Fedorchuk, Tatyana P., Vetoshkina, Daria V., Zhurikova, Elena M., Ignatova, Lyudmila K., Ivanov, Boris N.
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container_issue 1
container_start_page 69
container_title Protoplasma
container_volume 255
creator Rudenko, Natalia N.
Fedorchuk, Tatyana P.
Vetoshkina, Daria V.
Zhurikova, Elena M.
Ignatova, Lyudmila K.
Ivanov, Boris N.
description Effect of knockout of the At4g20990 gene encoding α-carbonic anhydrase 4 (α-CA4) in Arabidopsis thaliana in plants grown in low light (LL, 80 μmol photons m −2  s −1 ) or in high light (HL, 400 μmol photons m −2  s −1 ) under long (LD, 16 h) or short (SD, 8 h) day length was studied. In α-CA4 knockout plants, under all studied conditions, the non-photochemical quenching was lower; the decrease was more pronounced under HL. This pointed to α-CA4 implication in the processes leading to energy dissipation in PSII antenna. In this context the content of major antenna proteins Lhcb1 and Lhcb2 was lower in α-CA4 knockouts than in wild-type (WT) plants under all growth conditions. The expression level of lhcb2 gene was also lower in mutants grown under LD, LL and HL in comparison to WT. At the same time, this level was higher in mutants grown under SD, LL and it was the same under SD, HL. Overall, the data showed that the knockout of the At4g20990 gene affected both the contents of proteins of PSII light-harvesting complex and the expression level of genes encoding these proteins, with peculiarities dependent on day length. These data together with the fact of a decrease of non-photochemical quenching of leaf chlorophyll a fluorescence in α-CA4-mut as compared with that in WT plants implied that α-CA4 participates in acclimation of photosynthetic apparatus to light intensity, possibly playing important role in the photoprotection. The role of this CA can be especially important in plants growing under high illumination conditions.
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In α-CA4 knockout plants, under all studied conditions, the non-photochemical quenching was lower; the decrease was more pronounced under HL. This pointed to α-CA4 implication in the processes leading to energy dissipation in PSII antenna. In this context the content of major antenna proteins Lhcb1 and Lhcb2 was lower in α-CA4 knockouts than in wild-type (WT) plants under all growth conditions. The expression level of lhcb2 gene was also lower in mutants grown under LD, LL and HL in comparison to WT. At the same time, this level was higher in mutants grown under SD, LL and it was the same under SD, HL. Overall, the data showed that the knockout of the At4g20990 gene affected both the contents of proteins of PSII light-harvesting complex and the expression level of genes encoding these proteins, with peculiarities dependent on day length. These data together with the fact of a decrease of non-photochemical quenching of leaf chlorophyll a fluorescence in α-CA4-mut as compared with that in WT plants implied that α-CA4 participates in acclimation of photosynthetic apparatus to light intensity, possibly playing important role in the photoprotection. 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subjects Acclimation
Biomedical and Life Sciences
Carbonic anhydrases
Cell Biology
Chlorophyll
Growth conditions
Life Sciences
Light
Light intensity
Light-harvesting complex
Original Article
Photons
Photosynthetic apparatus
Photosystem II
Plant Sciences
Proteins
Zoology
title Influence of knockout of At4g20990 gene encoding α-CA4 on photosystem II light-harvesting antenna in plants grown under different light intensities and day lengths
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