Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells
The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle–cell interactions. In this study, pure human serum alb...
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| Veröffentlicht in: | Bioconjugate chemistry 2017-08, Vol.28 (8), p.2062-2068 |
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| container_title | Bioconjugate chemistry |
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| creator | Zyuzin, Mikhail V Yan, Yan Hartmann, Raimo Gause, Katelyn T Nazarenus, Moritz Cui, Jiwei Caruso, Frank Parak, Wolfgang J |
| description | The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle–cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. The ability to control the cellular recognition of these organic particles is expected to aid the advancement of HSA-based materials for intravenous drug delivery. |
| doi_str_mv | 10.1021/acs.bioconjchem.7b00231 |
| format | Article |
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However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle–cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. 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However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle–cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. The ability to control the cellular recognition of these organic particles is expected to aid the advancement of HSA-based materials for intravenous drug delivery.</description><subject>Albumin</subject><subject>Blood plasma</subject><subject>Cell interactions</subject><subject>Cells</subject><subject>Drug delivery</subject><subject>Drug delivery systems</subject><subject>Endosomes</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>Exposure</subject><subject>Human serum albumin</subject><subject>Human Umbilical Vein Endothelial Cells - metabolism</subject><subject>Humans</subject><subject>Intravenous administration</subject><subject>Lysosomes - metabolism</subject><subject>Nanomaterials</subject><subject>Nanopores</subject><subject>Nanotechnology</subject><subject>Particulates</subject><subject>Plasma</subject><subject>Plasmas (physics)</subject><subject>Protein Corona - chemistry</subject><subject>Protein Corona - metabolism</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Serum albumin</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - metabolism</subject><subject>Serum proteins</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Stability</subject><subject>Umbilical vein</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMozjj6Chpw46ba3Kqrajm0ozMwaONlXZwkJ0w1ubRJleJ7-MCm7faCGyFwsvj-7xz4CXnG2YozwV-CKSs9JZPiztxhWHWaMSH5PXLOW8Ea1XNxv_6Zkg3vmTgjj0rZMcYG3ouH5Ez0a6XWXJ2T7--TR5ocne-QbnOacYp0k3KKQF9hnr6gpS6nQK-XAJFuPZQA9MCg94uHTG_ijBnMPKVYqMb5K2KkbyGmfbUs5RT8gHkJ9NLrJdTwFvI8GY-FQrT0KtpU1_sJ_E9teUweOPAFn5zmBfn0-urj5rq5fffmZnN524CSam5a2zvGejMI3SmutbbScKYtFy20Sg8WUHVOA-Ncub6-wQG6wXImWy1hkBfkxdG7z-nzgmUew1RMvQAi1tNHPnApB9V1oqLP_0F3acmxXlcp2YpWifWB6o6UyamUjG7c5ylA_jZyNh6KG2tx41_FjafiavLpyb_ogPZ37ldTFZBH4GD4s_s_2h_8eaxc</recordid><startdate>20170816</startdate><enddate>20170816</enddate><creator>Zyuzin, Mikhail V</creator><creator>Yan, Yan</creator><creator>Hartmann, Raimo</creator><creator>Gause, Katelyn T</creator><creator>Nazarenus, Moritz</creator><creator>Cui, Jiwei</creator><creator>Caruso, Frank</creator><creator>Parak, Wolfgang J</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0197-497X</orcidid><orcidid>https://orcid.org/0000-0003-2938-4063</orcidid><orcidid>https://orcid.org/0000-0003-1672-6650</orcidid></search><sort><creationdate>20170816</creationdate><title>Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells</title><author>Zyuzin, Mikhail V ; 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Albumin Blood plasma Cell interactions Cells Drug delivery Drug delivery systems Endosomes Endothelial cells Endothelium Exposure Human serum albumin Human Umbilical Vein Endothelial Cells - metabolism Humans Intravenous administration Lysosomes - metabolism Nanomaterials Nanopores Nanotechnology Particulates Plasma Plasmas (physics) Protein Corona - chemistry Protein Corona - metabolism Protein Transport Proteins Serum albumin Serum Albumin - chemistry Serum Albumin - metabolism Serum proteins Silica Silicon dioxide Stability Umbilical vein |
| title | Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells |
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