Three-dimensional fine structure of the organization of microtubules in neurite varicosities by ultra-high voltage electron microscope tomography
Neurite varicosities are highly specialized compartments that are involved in neurotransmitter/ neuromodulator release and provide a physiological platform for neural functions. However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three...
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| Veröffentlicht in: | Cell and tissue research 2017-09, Vol.369 (3), p.467-476 |
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| creator | Nishida, Tomoki Yoshimura, Ryoichi Endo, Yasuhisa |
| description | Neurite varicosities are highly specialized compartments that are involved in neurotransmitter/ neuromodulator release and provide a physiological platform for neural functions. However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three-dimensional structure of microtubules in varicosities of a differentiated PC12 neural cell line using ultra-high voltage electron microscope tomography. Three-dimensional imaging showed that a part of the varicosities contained an accumulation of organelles that were separated from parallel microtubule arrays. Further detailed analysis using serial sections and whole-mount tomography revealed microtubules running in a spindle shape of swelling in some other types of varicosities. These electron tomographic results showed that the structural diversity and heterogeneity of microtubule organization supported the form of varicosities, suggesting that a different distribution pattern of microtubules in varicosities is crucial to the regulation of varicosities development. |
| doi_str_mv | 10.1007/s00441-017-2645-5 |
| format | Article |
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However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three-dimensional structure of microtubules in varicosities of a differentiated PC12 neural cell line using ultra-high voltage electron microscope tomography. Three-dimensional imaging showed that a part of the varicosities contained an accumulation of organelles that were separated from parallel microtubule arrays. Further detailed analysis using serial sections and whole-mount tomography revealed microtubules running in a spindle shape of swelling in some other types of varicosities. 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ultrastructure</subject><subject>Neuromodulation</subject><subject>Neurons</subject><subject>Organelles</subject><subject>PC12 Cells</subject><subject>Proteomics</subject><subject>Rats</subject><subject>Regular Article</subject><subject>Tomography</subject><subject>Ultrastructure</subject><subject>Voltage</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kt1qFTEUhQdR7LH6AN5IQBBvpuZvMnMuS7FVKHhTwbuQyeyZSckkx_wUTt_CNzbTU0srSggbdr61YO-sqnpL8AnBuP0UMeac1Ji0NRW8qZtn1YZwRmvctd3zaoMZpnUrxI-j6lWM1xgTLsT2ZXVEO8EFw2xT_bqaA0A9mAVcNN4pi0bjAMUUsk45APIjSnMpYVLO3KpUoLW3GB18yn22EJFxyEEOJgG6UcFoH00ypd_vUbYpqHo204xuvE1qAgQWdArF5s4jar8DlPzip6B28_519WJUNsKb-3pcfT__fHX2pb78dvH17PSy1rzFqR5G4KLH26EjhDSCUrIlPVNMs0brnjMClCpoGhg4CKxwq7pewTCqdmz12LTsuPp48N0F_zNDTHIxUYO1yoHPURY_xrZcUF7Q93-h1z6HsquVYpyIu_tATcqCNG70ZXK9msrTBtMWc0KbQp38gypngLIO72A0pf9E8OGRYAZl0xy9zetHxKcgOYDrUmOAUe6CWVTYS4Llmhd5yIsseZFrXuSqeXc_We4XGB4UfwJSAHoAYnlyE4RHo__X9TeYKsxl</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Nishida, Tomoki</creator><creator>Yoshimura, Ryoichi</creator><creator>Endo, Yasuhisa</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170901</creationdate><title>Three-dimensional fine structure of the organization of microtubules in neurite varicosities by ultra-high voltage electron microscope tomography</title><author>Nishida, Tomoki ; Yoshimura, Ryoichi ; Endo, Yasuhisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-dfe46b09d81115622191b3a3c35ccb431e22ae55ed4e60a07a8baedfa7f7cf573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cellular biology</topic><topic>Compartments</topic><topic>Detergents - pharmacology</topic><topic>Electron Microscope Tomography</topic><topic>Fine structure</topic><topic>Heterogeneity</topic><topic>High voltage</topic><topic>Human Genetics</topic><topic>Imaging, Three-Dimensional</topic><topic>Medical imaging</topic><topic>Microtubules</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - ultrastructure</topic><topic>Molecular Medicine</topic><topic>Neurites - drug effects</topic><topic>Neurites - ultrastructure</topic><topic>Neuromodulation</topic><topic>Neurons</topic><topic>Organelles</topic><topic>PC12 Cells</topic><topic>Proteomics</topic><topic>Rats</topic><topic>Regular Article</topic><topic>Tomography</topic><topic>Ultrastructure</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishida, Tomoki</creatorcontrib><creatorcontrib>Yoshimura, Ryoichi</creatorcontrib><creatorcontrib>Endo, Yasuhisa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishida, Tomoki</au><au>Yoshimura, Ryoichi</au><au>Endo, Yasuhisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three-dimensional fine structure of the organization of microtubules in neurite varicosities by ultra-high voltage electron microscope tomography</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>369</volume><issue>3</issue><spage>467</spage><epage>476</epage><pages>467-476</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Neurite varicosities are highly specialized compartments that are involved in neurotransmitter/ neuromodulator release and provide a physiological platform for neural functions. However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three-dimensional structure of microtubules in varicosities of a differentiated PC12 neural cell line using ultra-high voltage electron microscope tomography. Three-dimensional imaging showed that a part of the varicosities contained an accumulation of organelles that were separated from parallel microtubule arrays. Further detailed analysis using serial sections and whole-mount tomography revealed microtubules running in a spindle shape of swelling in some other types of varicosities. 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| subjects | Animals Biomedical and Life Sciences Biomedicine Cellular biology Compartments Detergents - pharmacology Electron Microscope Tomography Fine structure Heterogeneity High voltage Human Genetics Imaging, Three-Dimensional Medical imaging Microtubules Microtubules - drug effects Microtubules - ultrastructure Molecular Medicine Neurites - drug effects Neurites - ultrastructure Neuromodulation Neurons Organelles PC12 Cells Proteomics Rats Regular Article Tomography Ultrastructure Voltage |
| title | Three-dimensional fine structure of the organization of microtubules in neurite varicosities by ultra-high voltage electron microscope tomography |
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