Discovery and Characterization of Histidine Oxidation Initiated Cross-links in an IgG1 Monoclonal Antibody

Novel cross-links between an oxidized histidine and intact histidine, lysine, or cysteine residues were discovered and characterized from high-molecular weight (HMW) fractions of an IgG1 monoclonal antibody (mAb). The mAb HMW fractions were collected using preparative size-exclusion chromatography (...

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Veröffentlicht in:	Analytical chemistry (Washington) 2017-08, Vol.89 (15), p.7915-7923
Hauptverfasser:	Xu, Chong-Feng, Chen, Yunqiu, Yi, Linda, Brantley, Tim, Stanley, Brad, Sosic, Zoran, Zang, Li
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Carbonyls Chains Chemistry Chromatography Crosslinking Cysteine Fractions Histidine Immunoglobulin G Intermediates Links Lysine Mass spectrometry Mass spectroscopy Molecular weight Monoclonal antibodies Oxidation Peptide mapping Residues Size exclusion chromatography
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