Proteomic analysis of host cell protein dynamics in the supernatant of Fc‐fusion protein‐producing CHO DG44 and DUKX‐B11 cell lines in batch and fed‐batch cultures

ABSTRACT Chinese hamster ovary (CHO) cells are the most widely used host cell lines for the commercial production of therapeutic proteins including Fc‐fusion proteins. During the culture of recombinant CHO (rCHO) cells, host cell proteins (HCPs), secreted from viable cells and released from dead cel...

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Veröffentlicht in:	Biotechnology and bioengineering 2017-10, Vol.114 (10), p.2267-2278
Hauptverfasser:	Park, Jin Hyoung, Jin, Jong Hwa, Ji, In Jung, An, Hyun Joo, Kim, Jong Won, Lee, Gyun Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Batch Cell Culture Techniques - methods Batch culture Biotechnology Cell culture Cell fusion Cell lines Chinese hamster ovary cells CHO Cells - classification CHO Cells - physiology Cluster analysis Clustering Cricetulus Culture Media Fc receptors Fc‐fusion protein Fed batch Fed-batch culture Functional analysis Fusion protein Gene Expression Profiling - methods Genetic Engineering - methods host cell proteins Immunoglobulin Fc Fragments - chemistry Immunoglobulin Fc Fragments - metabolism Liquid chromatography Mass spectrometry Mass spectroscopy Peptide Mapping - methods product quality Protein purification Proteins Proteome - chemistry Proteome - metabolism proteomics Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry
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