SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection
Abstract Infections with the protozoan parasite Toxoplasma gondii , which are common around the world, can lead to congenital infections in humans. T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antige...
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| Veröffentlicht in: | Parasitology international 2017-10, Vol.66 (5), p.596-602 |
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| creator | Lu, Gang Zhou, Jian Zhou, Aihua Han, Yali Guo, Jingjing Song, Pengxia Zhou, Huaiyu Cong, Hua Hou, Ming Wang, Lin He, Shenyi |
| description | Abstract Infections with the protozoan parasite Toxoplasma gondii , which are common around the world, can lead to congenital infections in humans. T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antigenic DNA vaccine constructed to express T. gondii SAG5B and SAG5C proteins simultaneously was used to immunize BALB/c mice to evaluate the protective efficacy of the vaccine. IgG antibody and gamma interferon (IFN-γ) cytokine production in the pSAG5B/SAG5C DNA vaccine group were significantly higher (0.853 ± 0.103 and 915.2 ± 106.9, respectively) than in the single DNA vaccine groups (pSAG5B, 0.667 ± 0.109 and 598.3 ± 74.9, respectively; pSAG5C, 0.696 ± 0.092 and 623.7 ± 95.5, respectively). After a lethal challenge with 1 × 104 RH strain tachyzoites, the survival time of the mice (17 days) immunized with pSAG5B/SAG5C was longer than that of the single-gene-immunized mice (12 days) or the control mice (6 days). Moreover, after intragastric infection with 20 T. gondii PRU (low virulence) strain cysts, the number of brain cysts in the pSAG5B/SAG5C-vaccinated mice was only 25% of the number for the PBS-injected mice. Our findings indicate that, in comparison with the other mouse groups, the multi-antigenic DNA vaccine (pSAG5B/SAG5C) significantly induced immune responses and improved the protection against challenge with T. gondii in the host animals. |
| doi_str_mv | 10.1016/j.parint.2017.06.002 |
| format | Article |
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T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antigenic DNA vaccine constructed to express T. gondii SAG5B and SAG5C proteins simultaneously was used to immunize BALB/c mice to evaluate the protective efficacy of the vaccine. IgG antibody and gamma interferon (IFN-γ) cytokine production in the pSAG5B/SAG5C DNA vaccine group were significantly higher (0.853 ± 0.103 and 915.2 ± 106.9, respectively) than in the single DNA vaccine groups (pSAG5B, 0.667 ± 0.109 and 598.3 ± 74.9, respectively; pSAG5C, 0.696 ± 0.092 and 623.7 ± 95.5, respectively). After a lethal challenge with 1 × 104 RH strain tachyzoites, the survival time of the mice (17 days) immunized with pSAG5B/SAG5C was longer than that of the single-gene-immunized mice (12 days) or the control mice (6 days). Moreover, after intragastric infection with 20 T. gondii PRU (low virulence) strain cysts, the number of brain cysts in the pSAG5B/SAG5C-vaccinated mice was only 25% of the number for the PBS-injected mice. Our findings indicate that, in comparison with the other mouse groups, the multi-antigenic DNA vaccine (pSAG5B/SAG5C) significantly induced immune responses and improved the protection against challenge with T. gondii in the host animals.</description><identifier>ISSN: 1383-5769</identifier><identifier>EISSN: 1873-0329</identifier><identifier>DOI: 10.1016/j.parint.2017.06.002</identifier><identifier>PMID: 28602862</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Constructed plasmids ; Cytokines - biosynthesis ; Cytokines - blood ; Enzyme-Linked Immunosorbent Assay ; Gastroenterology and Hepatology ; Humans ; Immunity, Cellular ; Immunoglobulin G - blood ; Infectious Disease ; Interferon-gamma - biosynthesis ; Interferon-gamma - blood ; Mice ; Mice, Inbred BALB C ; Protozoan Proteins - administration & dosage ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Protozoan Vaccines - administration & dosage ; Protozoan Vaccines - immunology ; SAG5 ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis, Animal - prevention & control ; Vaccination ; Vaccine ; Vaccines, Combined - administration & dosage ; Vaccines, Combined - immunology ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology</subject><ispartof>Parasitology international, 2017-10, Vol.66 (5), p.596-602</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-bed1812980cd7a8f0e334603b3ae5ec41ad3d1e78f384e7e33031832fa568f163</citedby><cites>FETCH-LOGICAL-c507t-bed1812980cd7a8f0e334603b3ae5ec41ad3d1e78f384e7e33031832fa568f163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.parint.2017.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28602862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Gang</creatorcontrib><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Zhou, Aihua</creatorcontrib><creatorcontrib>Han, Yali</creatorcontrib><creatorcontrib>Guo, Jingjing</creatorcontrib><creatorcontrib>Song, Pengxia</creatorcontrib><creatorcontrib>Zhou, Huaiyu</creatorcontrib><creatorcontrib>Cong, Hua</creatorcontrib><creatorcontrib>Hou, Ming</creatorcontrib><creatorcontrib>Wang, Lin</creatorcontrib><creatorcontrib>He, Shenyi</creatorcontrib><title>SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection</title><title>Parasitology international</title><addtitle>Parasitol Int</addtitle><description>Abstract Infections with the protozoan parasite Toxoplasma gondii , which are common around the world, can lead to congenital infections in humans. T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antigenic DNA vaccine constructed to express T. gondii SAG5B and SAG5C proteins simultaneously was used to immunize BALB/c mice to evaluate the protective efficacy of the vaccine. IgG antibody and gamma interferon (IFN-γ) cytokine production in the pSAG5B/SAG5C DNA vaccine group were significantly higher (0.853 ± 0.103 and 915.2 ± 106.9, respectively) than in the single DNA vaccine groups (pSAG5B, 0.667 ± 0.109 and 598.3 ± 74.9, respectively; pSAG5C, 0.696 ± 0.092 and 623.7 ± 95.5, respectively). After a lethal challenge with 1 × 104 RH strain tachyzoites, the survival time of the mice (17 days) immunized with pSAG5B/SAG5C was longer than that of the single-gene-immunized mice (12 days) or the control mice (6 days). Moreover, after intragastric infection with 20 T. gondii PRU (low virulence) strain cysts, the number of brain cysts in the pSAG5B/SAG5C-vaccinated mice was only 25% of the number for the PBS-injected mice. Our findings indicate that, in comparison with the other mouse groups, the multi-antigenic DNA vaccine (pSAG5B/SAG5C) significantly induced immune responses and improved the protection against challenge with T. gondii in the host animals.</description><subject>Animals</subject><subject>Constructed plasmids</subject><subject>Cytokines - biosynthesis</subject><subject>Cytokines - blood</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gastroenterology and Hepatology</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious Disease</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - blood</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Protozoan Proteins - administration & dosage</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Protozoan Vaccines - administration & dosage</subject><subject>Protozoan Vaccines - immunology</subject><subject>SAG5</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - prevention & control</subject><subject>Vaccination</subject><subject>Vaccine</subject><subject>Vaccines, Combined - administration & dosage</subject><subject>Vaccines, Combined - immunology</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><issn>1383-5769</issn><issn>1873-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFO3DAQhi3UCijlDVDlYy9Jx_HGdi5IsKK0ElKl7vZsee0J8jaxFzuL4O3raIEDlx5G80v-Z8bzDSEXDGoGTHzb1juTfJjqBpisQdQAzRE5ZUryCnjTfSiaK161UnQn5FPOWwDWSsmOyUmjBJRoTsnv1dVte01NcHRWS2rjuPEBHX001hZBdylOaKdMR2-RmnvjQ57oOj7F3WDyaOh9DM576kNfbD6Gz-Rjb4aM5y_5jPz5frNe_qjuft3-XF7dVbYFOVUbdEyxplNgnTSqB-R8IYBvuMEW7YIZxx1DqXquFijLK3CmeNObVqieCX5Gvh76lh8-7DFPevTZ4jCYgHGfNetAyU4K6Ip1cbDaFHNO2Otd8qNJz5qBnmnqrT7Q1DNNDUIXmqXsy8uE_WZE91b0iq8YLg8GLHs-ekw6W4_BovOpwNAu-v9NeN_ADj54a4a_-Ix5G_cpFIaa6dxo0Kv5ovNBy_oASrX8H9TYm5I</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Lu, Gang</creator><creator>Zhou, Jian</creator><creator>Zhou, Aihua</creator><creator>Han, Yali</creator><creator>Guo, Jingjing</creator><creator>Song, Pengxia</creator><creator>Zhou, Huaiyu</creator><creator>Cong, Hua</creator><creator>Hou, Ming</creator><creator>Wang, Lin</creator><creator>He, Shenyi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20171001</creationdate><title>SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection</title><author>Lu, Gang ; Zhou, Jian ; Zhou, Aihua ; Han, Yali ; Guo, Jingjing ; Song, Pengxia ; Zhou, Huaiyu ; Cong, Hua ; Hou, Ming ; Wang, Lin ; He, Shenyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-bed1812980cd7a8f0e334603b3ae5ec41ad3d1e78f384e7e33031832fa568f163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Constructed plasmids</topic><topic>Cytokines - biosynthesis</topic><topic>Cytokines - blood</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gastroenterology and Hepatology</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Immunoglobulin G - blood</topic><topic>Infectious Disease</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - blood</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Protozoan Proteins - administration & dosage</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Protozoan Vaccines - administration & dosage</topic><topic>Protozoan Vaccines - immunology</topic><topic>SAG5</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - prevention & control</topic><topic>Vaccination</topic><topic>Vaccine</topic><topic>Vaccines, Combined - administration & dosage</topic><topic>Vaccines, Combined - immunology</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Gang</creatorcontrib><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Zhou, Aihua</creatorcontrib><creatorcontrib>Han, Yali</creatorcontrib><creatorcontrib>Guo, Jingjing</creatorcontrib><creatorcontrib>Song, Pengxia</creatorcontrib><creatorcontrib>Zhou, Huaiyu</creatorcontrib><creatorcontrib>Cong, Hua</creatorcontrib><creatorcontrib>Hou, Ming</creatorcontrib><creatorcontrib>Wang, Lin</creatorcontrib><creatorcontrib>He, Shenyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Gang</au><au>Zhou, Jian</au><au>Zhou, Aihua</au><au>Han, Yali</au><au>Guo, Jingjing</au><au>Song, Pengxia</au><au>Zhou, Huaiyu</au><au>Cong, Hua</au><au>Hou, Ming</au><au>Wang, Lin</au><au>He, Shenyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection</atitle><jtitle>Parasitology international</jtitle><addtitle>Parasitol Int</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>66</volume><issue>5</issue><spage>596</spage><epage>602</epage><pages>596-602</pages><issn>1383-5769</issn><eissn>1873-0329</eissn><abstract>Abstract Infections with the protozoan parasite Toxoplasma gondii , which are common around the world, can lead to congenital infections in humans. T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antigenic DNA vaccine constructed to express T. gondii SAG5B and SAG5C proteins simultaneously was used to immunize BALB/c mice to evaluate the protective efficacy of the vaccine. IgG antibody and gamma interferon (IFN-γ) cytokine production in the pSAG5B/SAG5C DNA vaccine group were significantly higher (0.853 ± 0.103 and 915.2 ± 106.9, respectively) than in the single DNA vaccine groups (pSAG5B, 0.667 ± 0.109 and 598.3 ± 74.9, respectively; pSAG5C, 0.696 ± 0.092 and 623.7 ± 95.5, respectively). After a lethal challenge with 1 × 104 RH strain tachyzoites, the survival time of the mice (17 days) immunized with pSAG5B/SAG5C was longer than that of the single-gene-immunized mice (12 days) or the control mice (6 days). Moreover, after intragastric infection with 20 T. gondii PRU (low virulence) strain cysts, the number of brain cysts in the pSAG5B/SAG5C-vaccinated mice was only 25% of the number for the PBS-injected mice. Our findings indicate that, in comparison with the other mouse groups, the multi-antigenic DNA vaccine (pSAG5B/SAG5C) significantly induced immune responses and improved the protection against challenge with T. gondii in the host animals.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28602862</pmid><doi>10.1016/j.parint.2017.06.002</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Constructed plasmids Cytokines - biosynthesis Cytokines - blood Enzyme-Linked Immunosorbent Assay Gastroenterology and Hepatology Humans Immunity, Cellular Immunoglobulin G - blood Infectious Disease Interferon-gamma - biosynthesis Interferon-gamma - blood Mice Mice, Inbred BALB C Protozoan Proteins - administration & dosage Protozoan Proteins - genetics Protozoan Proteins - immunology Protozoan Vaccines - administration & dosage Protozoan Vaccines - immunology SAG5 Toxoplasma - genetics Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis, Animal - prevention & control Vaccination Vaccine Vaccines, Combined - administration & dosage Vaccines, Combined - immunology Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology |
| title | SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection |
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