The first acidobacterial laccase-like multicopper oxidase revealed by metagenomics shows high salt and thermo-tolerance

Metagenomics is a powerful tool that allows identifying enzymes with novel properties from the unculturable component of microbiomes. However, thus far only a limited number of laccase or laccase -like enzymes identified through metagenomics has been subsequently biochemically characterized. This wo...

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Veröffentlicht in:	Applied microbiology and biotechnology 2017-08, Vol.101 (15), p.6261-6276
Hauptverfasser:	Ausec, Luka, Berini, Francesca, Casciello, Carmine, Cretoiu, Mariana Silvia, van Elsas, Jan Dirk, Marinelli, Flavia, Mandic-Mulec, Ines
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidic soils Acidobacteria - enzymology Acidobacteria - genetics Acidobacteria - metabolism Affinity Affinity chromatography Bacteria Bacterial Proteins - genetics Biochemistry Biomedical and Life Sciences Biotechnology Candidatus Solibacter Catalysis Catalytic activity Catechol Cloning, Molecular Decoloring Dihydroxyphenylalanine E coli Environmental Biotechnology Enzymes Escherichia coli Escherichia coli - genetics Fungi Gene Expression Genetic aspects Genetic testing Genomic Library Genomics Health aspects Hydrogen-Ion Concentration Laccase Laccase - genetics Laccase - metabolism Levodopa Libraries Life Sciences Lignin Metagenome Metagenomics Methods Microbial Genetics and Genomics Microbiology Mining Multicopper oxidase Organic solvents Oxidase Oxidases Oxidoreductases - genetics Oxidoreductases - isolation & purification Oxidoreductases - metabolism Phenolic compounds Phenols Pyrogallol Salt-Tolerance Similarity Sodium chloride Soil Microbiology Solvents Substrate Specificity Substrates Syringaldazine Temperature Vanillic acid
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