Chemical genomic analysis of GPR35 signaling
GPR35, a family A orphan G protein-coupled receptor, has been implicated in inflammatory, neurological, and cardiovascular diseases. However, not much is known about the signaling and functions of GPR35. We performed a label-free kinome short hairpin RNA screen and identified a putative signaling ne...
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| Veröffentlicht in: | Integrative biology (Cambridge) 2017-05, Vol.9 (5), p.451-463 |
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| creator | Hu, Heidi Haibei Deng, Huayun Ling, Shizhang Sun, Haiyan Kenakin, Terry Liang, Xinmiao Fang, Ye |
| description | GPR35, a family A orphan G protein-coupled receptor, has been implicated in inflammatory, neurological, and cardiovascular diseases. However, not much is known about the signaling and functions of GPR35. We performed a label-free kinome short hairpin RNA screen and identified a putative signaling network of GPR35 in HT-29 cells, some of which was validated using gene expression, biochemical and cellular assays. The results showed that GPR35 induced hypoxia-inducible factor 1α, and was involved in synaptic transmission, sensory perception, the immune system, and morphogenetic processes. Collectively, our data suggest that GPR35 may play an important role in response to hypoxic stress and be a potential target for the treatment of inflammatory, cardiovascular, and neurological disorders. |
| doi_str_mv | 10.1039/c7ib00005g |
| format | Article |
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| ispartof | Integrative biology (Cambridge), 2017-05, Vol.9 (5), p.451-463 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Gene Expression Profiling Genomics HT29 Cells Humans Hypoxia-Inducible Factor 1, alpha Subunit - biosynthesis Oligonucleotide Array Sequence Analysis Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - physiology RNA, Small Interfering - genetics Sensation Signal Transduction Synaptic Transmission |
| title | Chemical genomic analysis of GPR35 signaling |
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