Development of transgenic pigeonpea (Cajanus cajan. L Millsp) overexpressing citrate synthase gene for high phosphorus uptake

Plants have developed several adaptive strategies to enhance the availability and uptake of phosphorus (P) from the soil under conditions of P deficiency. Exudation of organic acids like citrate is one of the important strategies. In this study, we developed transgenic pigeonpea (Cajanus cajan) over...

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Veröffentlicht in:Indian journal of experimental biology 2016-08, Vol.54 (8), p.493-501
Hauptverfasser: Aftab Hussain, Aftab, Pavithra, I S, Sreevathsa, Rohini, Nataraja, K N, Babu, Naveen
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container_issue 8
container_start_page 493
container_title Indian journal of experimental biology
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creator Aftab Hussain, Aftab
Pavithra, I S
Sreevathsa, Rohini
Nataraja, K N
Babu, Naveen
description Plants have developed several adaptive strategies to enhance the availability and uptake of phosphorus (P) from the soil under conditions of P deficiency. Exudation of organic acids like citrate is one of the important strategies. In this study, we developed transgenic pigeonpea (Cajanus cajan) over-expressing Dacus carota citrate synthase (DcCs) gene to increase the synthesis and exudation of citrate. Transgenic plants were generated through agro bacterium mediated in-planta transformation technique. Integration and expression of the transgene was confirmed by genomic Southern and RT-PCR analysis. We observed that the transgenic lines had more tissue P and chlorophyll content, and also citrate synthase content higher in the roots. Further, transgenic lines had more vigorous root system both under P sufficient and deficient conditions with more lateral roots and root hairs under P deficient conditions. We conclude that the transgenic pigeonpea plants have the capacity to acquire more P under P deficient conditions.
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subjects Blotting, Southern
Cajanus - enzymology
Cajanus - genetics
Cajanus - growth & development
Chlorophyll - metabolism
Citrate (si)-Synthase - biosynthesis
Citrate (si)-Synthase - genetics
Enzyme Induction
Gene Expression Regulation, Plant
Genotype
Phenotype
Phosphorus - metabolism
Plant Roots - enzymology
Plant Roots - growth & development
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Plants, Genetically Modified - growth & development
Reverse Transcriptase Polymerase Chain Reaction
title Development of transgenic pigeonpea (Cajanus cajan. L Millsp) overexpressing citrate synthase gene for high phosphorus uptake
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