Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis
Purpose Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also th...
Gespeichert in:
| Veröffentlicht in: | Surgery today (Tokyo, Japan) Japan), 2018, Vol.48 (1), p.119-126 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 126 |
|---|---|
| container_issue | 1 |
| container_start_page | 119 |
| container_title | Surgery today (Tokyo, Japan) |
| container_volume | 48 |
| creator | Sakai, Rieko Maeda, Akira Choi, Thuy-Vy Lo, Pei-Chi Jiaravuthisan, Patmika Shabri, Afifah Mod Wang, Han-Tang Matsuura, Rei Kodama, Tasuku Eguchi, Hiroshi Okuyama, Hiroomi Miyagawa, Shuji |
| description | Purpose
Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also that of cellular xenogeneic rejection (CXR). Although macrophages have been well known to play a critical role in CXR, monocyte/macrophage-mediated xenogeneic rejection has not been well studied. In this study, we evaluated the effect of CD200 in xenogeneic rejection by macrophages.
Methods
Naïve swine endothelial cells (SEC) and SEC/CD200 were co-cultured with M0 macrophages and the cytotoxicity was measured by a WST-8 assay. The phagocytosis of SEC and SEC/CD200 by macrophages was analyzed by flow cytometry.
Results
While CD200 failed to suppress a significant amount of cytotoxicity against SEC by monocytes, M0 macrophage-mediated cytotoxicity was significantly suppressed by human CD200. The phagocytosis by M0 macrophages was also tested. The phagocytosis assay revealed that human CD200 suppresses M0 macrophage-mediated phagocytosis.
Conclusions
Our findings indicate that human CD200 suppresses the xenogeneic rejection by CD200R
+
macrophages and that the generation of hCD200 transgenic pigs for use in xenografts is very attractive for preventing the macrophage-mediated rejection. |
| doi_str_mv | 10.1007/s00595-017-1546-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1905732520</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1905732520</sourcerecordid><originalsourceid>FETCH-LOGICAL-p184t-3017bc1b63ef78c8ee33126cdbfb28b0f968f7b2033a9d207bca5f3bf73cddbe3</originalsourceid><addsrcrecordid>eNo1kEtPwzAQhC0EoqXwA7igHLkY1nYezhGVR5EqcQDOlu1sSqomDnYitf8eRy2nXe1-Gs0MIbcMHhhA8RgAsjKjwArKsjSn_IzMWSriIpk4J3MoU0YZL9mMXIWwBeCpBLgkMy6zQggu5-RzNba6S5bPHCAJY997DAFD0mrrXf-jN0hbrBo9YJXssXMb7LCxiT0MbnD7xjbDIdFdlUyom66hCdfkota7gDenuSDfry9fyxVdf7y9L5_WtGcyHaiIvo1lJhdYF9JKRCEYz21lasOlgbrMZV0YDkLosuIQYZ3VwtSFsFVlUCzI_VG39-53xDCotgkWdzvdoRuDYiXEmDyLCgtyd0JHE_Oo3jet9gf1X0QE-BEI8dVt0KutG30X7SsGampbHdtW0bWa2lZc_AEYO3FC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1905732520</pqid></control><display><type>article</type><title>Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Sakai, Rieko ; Maeda, Akira ; Choi, Thuy-Vy ; Lo, Pei-Chi ; Jiaravuthisan, Patmika ; Shabri, Afifah Mod ; Wang, Han-Tang ; Matsuura, Rei ; Kodama, Tasuku ; Eguchi, Hiroshi ; Okuyama, Hiroomi ; Miyagawa, Shuji</creator><creatorcontrib>Sakai, Rieko ; Maeda, Akira ; Choi, Thuy-Vy ; Lo, Pei-Chi ; Jiaravuthisan, Patmika ; Shabri, Afifah Mod ; Wang, Han-Tang ; Matsuura, Rei ; Kodama, Tasuku ; Eguchi, Hiroshi ; Okuyama, Hiroomi ; Miyagawa, Shuji</creatorcontrib><description>Purpose
Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also that of cellular xenogeneic rejection (CXR). Although macrophages have been well known to play a critical role in CXR, monocyte/macrophage-mediated xenogeneic rejection has not been well studied. In this study, we evaluated the effect of CD200 in xenogeneic rejection by macrophages.
Methods
Naïve swine endothelial cells (SEC) and SEC/CD200 were co-cultured with M0 macrophages and the cytotoxicity was measured by a WST-8 assay. The phagocytosis of SEC and SEC/CD200 by macrophages was analyzed by flow cytometry.
Results
While CD200 failed to suppress a significant amount of cytotoxicity against SEC by monocytes, M0 macrophage-mediated cytotoxicity was significantly suppressed by human CD200. The phagocytosis by M0 macrophages was also tested. The phagocytosis assay revealed that human CD200 suppresses M0 macrophage-mediated phagocytosis.
Conclusions
Our findings indicate that human CD200 suppresses the xenogeneic rejection by CD200R
+
macrophages and that the generation of hCD200 transgenic pigs for use in xenografts is very attractive for preventing the macrophage-mediated rejection.</description><identifier>ISSN: 0941-1291</identifier><identifier>EISSN: 1436-2813</identifier><identifier>DOI: 10.1007/s00595-017-1546-2</identifier><identifier>PMID: 28573328</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Animals ; Antigens, CD - physiology ; Cells, Cultured ; Cytotoxicity, Immunologic - genetics ; Endothelial Cells - immunology ; Flow Cytometry ; Graft Rejection - genetics ; Graft Rejection - immunology ; Humans ; Macrophages - immunology ; Medicine ; Medicine & Public Health ; Original Article ; Phagocytosis - genetics ; Surgery ; Surgical Oncology ; Swine</subject><ispartof>Surgery today (Tokyo, Japan), 2018, Vol.48 (1), p.119-126</ispartof><rights>Springer Japan 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p184t-3017bc1b63ef78c8ee33126cdbfb28b0f968f7b2033a9d207bca5f3bf73cddbe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00595-017-1546-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00595-017-1546-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28573328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakai, Rieko</creatorcontrib><creatorcontrib>Maeda, Akira</creatorcontrib><creatorcontrib>Choi, Thuy-Vy</creatorcontrib><creatorcontrib>Lo, Pei-Chi</creatorcontrib><creatorcontrib>Jiaravuthisan, Patmika</creatorcontrib><creatorcontrib>Shabri, Afifah Mod</creatorcontrib><creatorcontrib>Wang, Han-Tang</creatorcontrib><creatorcontrib>Matsuura, Rei</creatorcontrib><creatorcontrib>Kodama, Tasuku</creatorcontrib><creatorcontrib>Eguchi, Hiroshi</creatorcontrib><creatorcontrib>Okuyama, Hiroomi</creatorcontrib><creatorcontrib>Miyagawa, Shuji</creatorcontrib><title>Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis</title><title>Surgery today (Tokyo, Japan)</title><addtitle>Surg Today</addtitle><addtitle>Surg Today</addtitle><description>Purpose
Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also that of cellular xenogeneic rejection (CXR). Although macrophages have been well known to play a critical role in CXR, monocyte/macrophage-mediated xenogeneic rejection has not been well studied. In this study, we evaluated the effect of CD200 in xenogeneic rejection by macrophages.
Methods
Naïve swine endothelial cells (SEC) and SEC/CD200 were co-cultured with M0 macrophages and the cytotoxicity was measured by a WST-8 assay. The phagocytosis of SEC and SEC/CD200 by macrophages was analyzed by flow cytometry.
Results
While CD200 failed to suppress a significant amount of cytotoxicity against SEC by monocytes, M0 macrophage-mediated cytotoxicity was significantly suppressed by human CD200. The phagocytosis by M0 macrophages was also tested. The phagocytosis assay revealed that human CD200 suppresses M0 macrophage-mediated phagocytosis.
Conclusions
Our findings indicate that human CD200 suppresses the xenogeneic rejection by CD200R
+
macrophages and that the generation of hCD200 transgenic pigs for use in xenografts is very attractive for preventing the macrophage-mediated rejection.</description><subject>Animals</subject><subject>Antigens, CD - physiology</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity, Immunologic - genetics</subject><subject>Endothelial Cells - immunology</subject><subject>Flow Cytometry</subject><subject>Graft Rejection - genetics</subject><subject>Graft Rejection - immunology</subject><subject>Humans</subject><subject>Macrophages - immunology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Phagocytosis - genetics</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Swine</subject><issn>0941-1291</issn><issn>1436-2813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtPwzAQhC0EoqXwA7igHLkY1nYezhGVR5EqcQDOlu1sSqomDnYitf8eRy2nXe1-Gs0MIbcMHhhA8RgAsjKjwArKsjSn_IzMWSriIpk4J3MoU0YZL9mMXIWwBeCpBLgkMy6zQggu5-RzNba6S5bPHCAJY997DAFD0mrrXf-jN0hbrBo9YJXssXMb7LCxiT0MbnD7xjbDIdFdlUyom66hCdfkota7gDenuSDfry9fyxVdf7y9L5_WtGcyHaiIvo1lJhdYF9JKRCEYz21lasOlgbrMZV0YDkLosuIQYZ3VwtSFsFVlUCzI_VG39-53xDCotgkWdzvdoRuDYiXEmDyLCgtyd0JHE_Oo3jet9gf1X0QE-BEI8dVt0KutG30X7SsGampbHdtW0bWa2lZc_AEYO3FC</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Sakai, Rieko</creator><creator>Maeda, Akira</creator><creator>Choi, Thuy-Vy</creator><creator>Lo, Pei-Chi</creator><creator>Jiaravuthisan, Patmika</creator><creator>Shabri, Afifah Mod</creator><creator>Wang, Han-Tang</creator><creator>Matsuura, Rei</creator><creator>Kodama, Tasuku</creator><creator>Eguchi, Hiroshi</creator><creator>Okuyama, Hiroomi</creator><creator>Miyagawa, Shuji</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2018</creationdate><title>Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis</title><author>Sakai, Rieko ; Maeda, Akira ; Choi, Thuy-Vy ; Lo, Pei-Chi ; Jiaravuthisan, Patmika ; Shabri, Afifah Mod ; Wang, Han-Tang ; Matsuura, Rei ; Kodama, Tasuku ; Eguchi, Hiroshi ; Okuyama, Hiroomi ; Miyagawa, Shuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p184t-3017bc1b63ef78c8ee33126cdbfb28b0f968f7b2033a9d207bca5f3bf73cddbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antigens, CD - physiology</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity, Immunologic - genetics</topic><topic>Endothelial Cells - immunology</topic><topic>Flow Cytometry</topic><topic>Graft Rejection - genetics</topic><topic>Graft Rejection - immunology</topic><topic>Humans</topic><topic>Macrophages - immunology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Phagocytosis - genetics</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakai, Rieko</creatorcontrib><creatorcontrib>Maeda, Akira</creatorcontrib><creatorcontrib>Choi, Thuy-Vy</creatorcontrib><creatorcontrib>Lo, Pei-Chi</creatorcontrib><creatorcontrib>Jiaravuthisan, Patmika</creatorcontrib><creatorcontrib>Shabri, Afifah Mod</creatorcontrib><creatorcontrib>Wang, Han-Tang</creatorcontrib><creatorcontrib>Matsuura, Rei</creatorcontrib><creatorcontrib>Kodama, Tasuku</creatorcontrib><creatorcontrib>Eguchi, Hiroshi</creatorcontrib><creatorcontrib>Okuyama, Hiroomi</creatorcontrib><creatorcontrib>Miyagawa, Shuji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Surgery today (Tokyo, Japan)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakai, Rieko</au><au>Maeda, Akira</au><au>Choi, Thuy-Vy</au><au>Lo, Pei-Chi</au><au>Jiaravuthisan, Patmika</au><au>Shabri, Afifah Mod</au><au>Wang, Han-Tang</au><au>Matsuura, Rei</au><au>Kodama, Tasuku</au><au>Eguchi, Hiroshi</au><au>Okuyama, Hiroomi</au><au>Miyagawa, Shuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis</atitle><jtitle>Surgery today (Tokyo, Japan)</jtitle><stitle>Surg Today</stitle><addtitle>Surg Today</addtitle><date>2018</date><risdate>2018</risdate><volume>48</volume><issue>1</issue><spage>119</spage><epage>126</epage><pages>119-126</pages><issn>0941-1291</issn><eissn>1436-2813</eissn><abstract>Purpose
Various strategies, such as the generation of alpha-1,3-galactosyltransferase knocked-out pigs and CD55 transgenic pigs, have been investigated to inhibit pig to human xenogeneic rejection. Our aim is to develop strategies to overcome the hurdle of not only hyper acute rejection, but also that of cellular xenogeneic rejection (CXR). Although macrophages have been well known to play a critical role in CXR, monocyte/macrophage-mediated xenogeneic rejection has not been well studied. In this study, we evaluated the effect of CD200 in xenogeneic rejection by macrophages.
Methods
Naïve swine endothelial cells (SEC) and SEC/CD200 were co-cultured with M0 macrophages and the cytotoxicity was measured by a WST-8 assay. The phagocytosis of SEC and SEC/CD200 by macrophages was analyzed by flow cytometry.
Results
While CD200 failed to suppress a significant amount of cytotoxicity against SEC by monocytes, M0 macrophage-mediated cytotoxicity was significantly suppressed by human CD200. The phagocytosis by M0 macrophages was also tested. The phagocytosis assay revealed that human CD200 suppresses M0 macrophage-mediated phagocytosis.
Conclusions
Our findings indicate that human CD200 suppresses the xenogeneic rejection by CD200R
+
macrophages and that the generation of hCD200 transgenic pigs for use in xenografts is very attractive for preventing the macrophage-mediated rejection.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>28573328</pmid><doi>10.1007/s00595-017-1546-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0941-1291 |
| ispartof | Surgery today (Tokyo, Japan), 2018, Vol.48 (1), p.119-126 |
| issn | 0941-1291 1436-2813 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1905732520 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antigens, CD - physiology Cells, Cultured Cytotoxicity, Immunologic - genetics Endothelial Cells - immunology Flow Cytometry Graft Rejection - genetics Graft Rejection - immunology Humans Macrophages - immunology Medicine Medicine & Public Health Original Article Phagocytosis - genetics Surgery Surgical Oncology Swine |
| title | Human CD200 suppresses macrophage-mediated xenogeneic cytotoxicity and phagocytosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T02%3A59%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20CD200%20suppresses%20macrophage-mediated%20xenogeneic%20cytotoxicity%20and%20phagocytosis&rft.jtitle=Surgery%20today%20(Tokyo,%20Japan)&rft.au=Sakai,%20Rieko&rft.date=2018&rft.volume=48&rft.issue=1&rft.spage=119&rft.epage=126&rft.pages=119-126&rft.issn=0941-1291&rft.eissn=1436-2813&rft_id=info:doi/10.1007/s00595-017-1546-2&rft_dat=%3Cproquest_pubme%3E1905732520%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1905732520&rft_id=info:pmid/28573328&rfr_iscdi=true |