MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin
Objective To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145. Results In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin m...
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| Veröffentlicht in: | Biotechnology letters 2017-05, Vol.39 (5), p.701-710 |
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| container_title | Biotechnology letters |
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| creator | Mo, Dongping Yang, Daheng Xiao, Xuelian Sun, Ruihong Huang, Lei Xu, Jian |
| description | Objective
To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
Results
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
Conclusions
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin. |
| doi_str_mv | 10.1007/s10529-017-2290-9 |
| format | Article |
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To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
Results
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
Conclusions
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-017-2290-9</identifier><identifier>PMID: 28120164</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>A549 Cells ; Adenocarcinoma - epidemiology ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Adenocarcinoma of Lung ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Applied Microbiology ; Binding ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cadherins - genetics ; Cadherins - metabolism ; Cell Movement - genetics ; Cohort Studies ; Control ; Correlation ; Female ; Gene expression ; Gene Knockdown Techniques ; Humans ; Life Sciences ; Lung Neoplasms - epidemiology ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Lungs ; Lymph ; Male ; Microbiology ; MicroRNAs - analysis ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; Migration ; Neoplasm Invasiveness - genetics ; Original Research Paper</subject><ispartof>Biotechnology letters, 2017-05, Vol.39 (5), p.701-710</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Biotechnology Letters is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-f4eface153a91ff92cadcbd1d2927f14f0d2e65a7d43356d15d55738513b38e73</citedby><cites>FETCH-LOGICAL-c475t-f4eface153a91ff92cadcbd1d2927f14f0d2e65a7d43356d15d55738513b38e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-017-2290-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-017-2290-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28120164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mo, Dongping</creatorcontrib><creatorcontrib>Yang, Daheng</creatorcontrib><creatorcontrib>Xiao, Xuelian</creatorcontrib><creatorcontrib>Sun, Ruihong</creatorcontrib><creatorcontrib>Huang, Lei</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><title>MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Objective
To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
Results
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
Conclusions
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.</description><subject>A549 Cells</subject><subject>Adenocarcinoma - epidemiology</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Adenocarcinoma of Lung</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Applied Microbiology</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Cell Movement - genetics</subject><subject>Cohort Studies</subject><subject>Control</subject><subject>Correlation</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Lung Neoplasms - epidemiology</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Lungs</subject><subject>Lymph</subject><subject>Male</subject><subject>Microbiology</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>Migration</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Original Research Paper</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV1r2zAUhsVoWdJ0P6A3xbCb3WjV0YdlXZbSboM0hdLeDYRiyalCLGeSXci_n7xkYxRKCwIh9LyPdHgROgPyFQiRFwmIoAoTkJhSRbD6gKYgJMOllOURmhLggAVXdIJOUloTQpQk8iOa0AoogZJP0c9bf7-4xMBFkYbtNrqUXCo2Q1gVxrrQ1SbWPnStKWq32RQ-PJvku1CYYIvWr6Lpx9NyV_Qmrlzvc26Ba2OfXPThFB03ZpPcp8M-Q4831w9X3_H87tuPq8s5rrkUPW64a0ztQDCjoGkUzfl6acFSRWUDvCGWulIYaTljorQgrMhTVgLYklVOshn6svduY_drcKnXrU_jf01w3ZA0KMIpFxUlb6OVkkwqWYp3oGVeFftj_fwCXXdDDHnmUUiFFKVimYI9VccupegavY2-NXGngeixUL0vVOdC9VioVjlzfjAPy9bZf4m_DWaA7oGUr8LKxf-eftX6G5O0qV4</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Mo, Dongping</creator><creator>Yang, Daheng</creator><creator>Xiao, Xuelian</creator><creator>Sun, Ruihong</creator><creator>Huang, Lei</creator><creator>Xu, Jian</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20170501</creationdate><title>MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin</title><author>Mo, Dongping ; Yang, Daheng ; Xiao, Xuelian ; Sun, Ruihong ; Huang, Lei ; Xu, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-f4eface153a91ff92cadcbd1d2927f14f0d2e65a7d43356d15d55738513b38e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>A549 Cells</topic><topic>Adenocarcinoma - epidemiology</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Adenocarcinoma of Lung</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Applied Microbiology</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Cell Movement - genetics</topic><topic>Cohort Studies</topic><topic>Control</topic><topic>Correlation</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Lung Neoplasms - epidemiology</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Lungs</topic><topic>Lymph</topic><topic>Male</topic><topic>Microbiology</topic><topic>MicroRNAs - analysis</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>Migration</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Original Research Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mo, Dongping</creatorcontrib><creatorcontrib>Yang, Daheng</creatorcontrib><creatorcontrib>Xiao, Xuelian</creatorcontrib><creatorcontrib>Sun, Ruihong</creatorcontrib><creatorcontrib>Huang, Lei</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mo, Dongping</au><au>Yang, Daheng</au><au>Xiao, Xuelian</au><au>Sun, Ruihong</au><au>Huang, Lei</au><au>Xu, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>39</volume><issue>5</issue><spage>701</spage><epage>710</epage><pages>701-710</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objective
To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
Results
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
Conclusions
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28120164</pmid><doi>10.1007/s10529-017-2290-9</doi><tpages>10</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2017-05, Vol.39 (5), p.701-710 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | A549 Cells Adenocarcinoma - epidemiology Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Adenocarcinoma of Lung Antigens, CD - genetics Antigens, CD - metabolism Applied Microbiology Binding Biochemistry Biomedical and Life Sciences Biotechnology Cadherins - genetics Cadherins - metabolism Cell Movement - genetics Cohort Studies Control Correlation Female Gene expression Gene Knockdown Techniques Humans Life Sciences Lung Neoplasms - epidemiology Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Lungs Lymph Male Microbiology MicroRNAs - analysis MicroRNAs - genetics MicroRNAs - metabolism Middle Aged Migration Neoplasm Invasiveness - genetics Original Research Paper |
| title | MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin |
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