Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes
For pyruvate‐producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production i...
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| Veröffentlicht in: | Letters in applied microbiology 2017-08, Vol.65 (2), p.165-172 |
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| creator | Yang, M. Mu, T. Zhong, W. Olajuyin, A.M. Xing, J. |
| description | For pyruvate‐producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome‐wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner‐Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate‐producing strains.
Significance and Impact of the Study
Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.
Significance and Impact of the Study: Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production. |
| doi_str_mv | 10.1111/lam.12758 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1904234056</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1917788122</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-b22b96da2d0e1466a46d1a7284b892813ad25bb6f4f7a88d993e64f09e9bdd2a3</originalsourceid><addsrcrecordid>eNp10U9LHDEYBvAgFt2qB7-ABHppD6NJJpPJHJdFrbCllxa8DfnzzhqZSdZkRtlDwY_gZ-wnabarPRTMJfDyy0N4H4ROKTmn-Vz0ajinrK7kHppRXrOiFtXtPpoRJkQhWcUP0ceU7gkhkrLmAB0yWQnKBJmhX3Ov-k1yCYcOr_rJBK9GwAOMSofepQF3IeL1Jk6P2_k6BjuZ0QWPncfgV84DRLD4Mpk7iM7cOYVNfoi1Snmc3Qp8GOD388uTs4DHqHwy0a3HPEzH6EOn-gQnr_cR-nl1-WPxtVh-v75ZzJeFKatSFpox3QirmCVAuRCKC0tVzSTXsmGSlsqySmvR8a5WUtqmKUHwjjTQaGuZKo_Q511u_v_DBGlsB5cM9L3yEKbU0oZwVnJSiUw__UfvwxTzkraK1rXMK2RZfdkpE0NKEbp2Hd2g4qalpN120uZO2r-dZHv2mjjpAew_-VZCBhc78OR62Lyf1C7n33aRfwBOTJjM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1917788122</pqid></control><display><type>article</type><title>Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Yang, M. ; Mu, T. ; Zhong, W. ; Olajuyin, A.M. ; Xing, J.</creator><creatorcontrib>Yang, M. ; Mu, T. ; Zhong, W. ; Olajuyin, A.M. ; Xing, J.</creatorcontrib><description>For pyruvate‐producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome‐wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner‐Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate‐producing strains.
Significance and Impact of the Study
Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.
Significance and Impact of the Study: Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.12758</identifier><identifier>PMID: 28561260</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenine ; Carbon ; Carbon sources ; Construction ; E coli ; Entner-Doudoroff pathway ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Gene expression ; Genes ; Genomes ; genome‐wide transcriptome ; gluconate metabolism ; Gluconates - metabolism ; Glucose ; Glucose - metabolism ; Glycerol ; Glycolysis ; Metabolic Networks and Pathways ; Metabolic pathways ; Metabolism ; NAD ; NADH ; Nicotinamide ; Nicotinamide adenine dinucleotide ; Organisms, Genetically Modified ; Phosphorylation ; pyruvate ; Pyruvic acid ; Pyruvic Acid - metabolism ; redox state ; Sodium ; Sodium gluconate ; Synthesis ; Transcription ; Transcriptome</subject><ispartof>Letters in applied microbiology, 2017-08, Vol.65 (2), p.165-172</ispartof><rights>2017 The Society for Applied Microbiology</rights><rights>2017 The Society for Applied Microbiology.</rights><rights>Copyright © 2017 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-b22b96da2d0e1466a46d1a7284b892813ad25bb6f4f7a88d993e64f09e9bdd2a3</citedby><cites>FETCH-LOGICAL-c3538-b22b96da2d0e1466a46d1a7284b892813ad25bb6f4f7a88d993e64f09e9bdd2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.12758$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.12758$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28561260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, M.</creatorcontrib><creatorcontrib>Mu, T.</creatorcontrib><creatorcontrib>Zhong, W.</creatorcontrib><creatorcontrib>Olajuyin, A.M.</creatorcontrib><creatorcontrib>Xing, J.</creatorcontrib><title>Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>For pyruvate‐producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome‐wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner‐Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate‐producing strains.
Significance and Impact of the Study
Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.
Significance and Impact of the Study: Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.</description><subject>Adenine</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Construction</subject><subject>E coli</subject><subject>Entner-Doudoroff pathway</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>genome‐wide transcriptome</subject><subject>gluconate metabolism</subject><subject>Gluconates - metabolism</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glycerol</subject><subject>Glycolysis</subject><subject>Metabolic Networks and Pathways</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>NAD</subject><subject>NADH</subject><subject>Nicotinamide</subject><subject>Nicotinamide adenine dinucleotide</subject><subject>Organisms, Genetically Modified</subject><subject>Phosphorylation</subject><subject>pyruvate</subject><subject>Pyruvic acid</subject><subject>Pyruvic Acid - metabolism</subject><subject>redox state</subject><subject>Sodium</subject><subject>Sodium gluconate</subject><subject>Synthesis</subject><subject>Transcription</subject><subject>Transcriptome</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U9LHDEYBvAgFt2qB7-ABHppD6NJJpPJHJdFrbCllxa8DfnzzhqZSdZkRtlDwY_gZ-wnabarPRTMJfDyy0N4H4ROKTmn-Vz0ajinrK7kHppRXrOiFtXtPpoRJkQhWcUP0ceU7gkhkrLmAB0yWQnKBJmhX3Ov-k1yCYcOr_rJBK9GwAOMSofepQF3IeL1Jk6P2_k6BjuZ0QWPncfgV84DRLD4Mpk7iM7cOYVNfoi1Snmc3Qp8GOD388uTs4DHqHwy0a3HPEzH6EOn-gQnr_cR-nl1-WPxtVh-v75ZzJeFKatSFpox3QirmCVAuRCKC0tVzSTXsmGSlsqySmvR8a5WUtqmKUHwjjTQaGuZKo_Q511u_v_DBGlsB5cM9L3yEKbU0oZwVnJSiUw__UfvwxTzkraK1rXMK2RZfdkpE0NKEbp2Hd2g4qalpN120uZO2r-dZHv2mjjpAew_-VZCBhc78OR62Lyf1C7n33aRfwBOTJjM</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Yang, M.</creator><creator>Mu, T.</creator><creator>Zhong, W.</creator><creator>Olajuyin, A.M.</creator><creator>Xing, J.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes</title><author>Yang, M. ; Mu, T. ; Zhong, W. ; Olajuyin, A.M. ; Xing, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-b22b96da2d0e1466a46d1a7284b892813ad25bb6f4f7a88d993e64f09e9bdd2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenine</topic><topic>Carbon</topic><topic>Carbon sources</topic><topic>Construction</topic><topic>E coli</topic><topic>Entner-Doudoroff pathway</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomes</topic><topic>genome‐wide transcriptome</topic><topic>gluconate metabolism</topic><topic>Gluconates - metabolism</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Glycerol</topic><topic>Glycolysis</topic><topic>Metabolic Networks and Pathways</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>NAD</topic><topic>NADH</topic><topic>Nicotinamide</topic><topic>Nicotinamide adenine dinucleotide</topic><topic>Organisms, Genetically Modified</topic><topic>Phosphorylation</topic><topic>pyruvate</topic><topic>Pyruvic acid</topic><topic>Pyruvic Acid - metabolism</topic><topic>redox state</topic><topic>Sodium</topic><topic>Sodium gluconate</topic><topic>Synthesis</topic><topic>Transcription</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, M.</creatorcontrib><creatorcontrib>Mu, T.</creatorcontrib><creatorcontrib>Zhong, W.</creatorcontrib><creatorcontrib>Olajuyin, A.M.</creatorcontrib><creatorcontrib>Xing, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, M.</au><au>Mu, T.</au><au>Zhong, W.</au><au>Olajuyin, A.M.</au><au>Xing, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2017-08</date><risdate>2017</risdate><volume>65</volume><issue>2</issue><spage>165</spage><epage>172</epage><pages>165-172</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><abstract>For pyruvate‐producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome‐wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner‐Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate‐producing strains.
Significance and Impact of the Study
Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.
Significance and Impact of the Study: Comparing with glucose, using sodium gluconate as carbon source brought a decrease in nicotinamide adenine dinucleotide and an increase in pyruvate production in engineered strain YP211. From the genome‐wide transcriptome analysis, the Entner‐Doudoroff pathway was activated strongly in gluconate metabolism, which innovatively provided a shorter and more effective pathway for pyruvate production.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>28561260</pmid><doi>10.1111/lam.12758</doi><tpages>8</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library - AutoHoldings Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenine Carbon Carbon sources Construction E coli Entner-Doudoroff pathway Escherichia coli - genetics Escherichia coli - metabolism Gene expression Genes Genomes genome‐wide transcriptome gluconate metabolism Gluconates - metabolism Glucose Glucose - metabolism Glycerol Glycolysis Metabolic Networks and Pathways Metabolic pathways Metabolism NAD NADH Nicotinamide Nicotinamide adenine dinucleotide Organisms, Genetically Modified Phosphorylation pyruvate Pyruvic acid Pyruvic Acid - metabolism redox state Sodium Sodium gluconate Synthesis Transcription Transcriptome |
| title | Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome‐wide transcriptomes |
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