Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice

FHIRM-TPM is a miniature two-photon microscope capable of imaging fluorescently labeled neurons in the brains of freely behaving mice. It allows for imaging of spines or recording of neural activity with a frame rate up to 40 Hz. Developments in miniaturized microscopes have enabled visualization of...

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Veröffentlicht in:	Nature methods 2017-07, Vol.14 (7), p.713-719
Hauptverfasser:	Zong, Weijian, Wu, Runlong, Li, Mingli, Hu, Yanhui, Li, Yijun, Li, Jinghang, Rong, Hao, Wu, Haitao, Xu, Yangyang, Lu, Yang, Jia, Hongbo, Fan, Ming, Zhou, Zhuan, Zhang, Yunfeng, Wang, Aimin, Chen, Liangyi, Cheng, Heping
Format:	Artikel
Sprache:	eng
Schlagworte:	14/35 14/63 14/69 631/1647/245/2225 631/1647/328/2057 631/1647/328/2235 631/1647/334/1874/345 631/378 64/60 Animal behavior Animals Behavior, Animal Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biosensors Brain Brain - cytology Brain - diagnostic imaging Brain - physiology Cytological Techniques - methods Dendrites Dendritic spines Femtosecond pulses High resolution Innovations Life Sciences Medical imaging Mice Microscopes Microscopy Microscopy, Fluorescence, Multiphoton - methods Miniaturization - methods Neuroimaging Observations Photonic crystals Proteomics Raster scanning Robustness Rodents Scanning Synaptic transmission Weighing
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