The photostability of the commonly used biotin-4-fluorescein probe

Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined number of biotin binding sites....

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Veröffentlicht in:	Analytical biochemistry 2017-08, Vol.531, p.78-82
Hauptverfasser:	Haack, Richard A., Swift, Kerry M., Ruan, Qiaoqiao, Himmelsbach, Richard J., Tetin, Sergey Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotin - analogs & derivatives Biotin - chemistry Biotinylation Fluorescein Fluoresceins - chemistry Fluorophore Oxidation-Reduction Photochemical Processes Photosensitizer Strept(avidin) Streptavidin - chemistry Sulfoxide
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