Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus
Background This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA‐Sal) to detect HBV DNA by qualitative PCR. Materials and Methods Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐ho...
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| Veröffentlicht in: | Oral diseases 2017-11, Vol.23 (8), p.1072-1079 |
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| creator | Portilho, MM Mendonça, ACF Marques, VA Nabuco, LC Villela‐Nogueira, CA Ivantes, CAP Lewis‐Ximenez, LL Lampe, E Villar, LM |
| description | Background
This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA‐Sal) to detect HBV DNA by qualitative PCR.
Materials and Methods
Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum.
Results
HBV DNA was detected in all serum samples from HBV‐infected individuals, and it was not detected in control group. HBV DNA from HBV group was detected in 17 samples collected with Salivette device, 16 samples collected by FTA Card device, 16 samples collected from spitting and 13 samples collected by DNA‐Sal device. Samples that corresponded to a higher viral load in their paired serum sample could be detected using all oral fluid collection methods, but Salivette collection device yielded the largest numbers of positive samples and had a wide range of viral load that was detected.
Conclusion
It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples. |
| doi_str_mv | 10.1111/odi.12692 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1903166134</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1949588474</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3532-ac6cbf9b9c45349b28fefb86511ad323da5e1732c23f52b5687c4d2db0d1aada3</originalsourceid><addsrcrecordid>eNp10ElLAzEYBuAgivvBPyABL3qYmnWWo9YVCr0oeBBCJguNZJqazCj996a2ehDMJYHvycvHC8AJRiOcz2XQboRJ2ZAtsI9LhAtUE76d35SzghP6sgcOUnpDCFcNJbtgj9ScMcSqffA6Dt1CRpfCHAYLQ5QeWj84DVXw3qje5UFn-lnQCdoQYT8zsAt5MngZoTb9xuTPM7OQvetdgtfww8UhHYEdK30yx5v7EDzf3T6NH4rJ9P5xfDUpFOWUFFKVqrVN2yjGKWtaUltj27rkGEtNCdWSG1xRogi1nLS8rCvFNNEt0lhKLekhOF_nLmJ4H0zqReeSMt7LuQlDErhBFJclpizTsz_0LQxxnrfLijW8rlm1UhdrpWJIKRorFtF1Mi4FRmJVuciVi-_Ksz3dJA5tZ_Sv_Ok4g8s1-HTeLP9PEtObx3XkF005iy4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1949588474</pqid></control><display><type>article</type><title>Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Portilho, MM ; Mendonça, ACF ; Marques, VA ; Nabuco, LC ; Villela‐Nogueira, CA ; Ivantes, CAP ; Lewis‐Ximenez, LL ; Lampe, E ; Villar, LM</creator><creatorcontrib>Portilho, MM ; Mendonça, ACF ; Marques, VA ; Nabuco, LC ; Villela‐Nogueira, CA ; Ivantes, CAP ; Lewis‐Ximenez, LL ; Lampe, E ; Villar, LM</creatorcontrib><description>Background
This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA‐Sal) to detect HBV DNA by qualitative PCR.
Materials and Methods
Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum.
Results
HBV DNA was detected in all serum samples from HBV‐infected individuals, and it was not detected in control group. HBV DNA from HBV group was detected in 17 samples collected with Salivette device, 16 samples collected by FTA Card device, 16 samples collected from spitting and 13 samples collected by DNA‐Sal device. Samples that corresponded to a higher viral load in their paired serum sample could be detected using all oral fluid collection methods, but Salivette collection device yielded the largest numbers of positive samples and had a wide range of viral load that was detected.
Conclusion
It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12692</identifier><identifier>PMID: 28544047</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adolescent ; Adult ; Aged ; Dentistry ; Deoxyribonucleic acid ; DNA ; DNA, Viral - analysis ; DNA, Viral - blood ; DNA, Viral - isolation & purification ; Female ; Fish ; Hepatitis B ; Hepatitis B - blood ; Hepatitis B - diagnosis ; Hepatitis B virus - genetics ; Humans ; Male ; Middle Aged ; molecular detection ; oral fluid ; Polymerase chain reaction ; Saliva - chemistry ; Saliva - virology ; Specimen Handling - methods ; Viral Load ; Young Adult</subject><ispartof>Oral diseases, 2017-11, Vol.23 (8), p.1072-1079</ispartof><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2017 John Wiley & Sons A/S. Published by John Wiley &Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-ac6cbf9b9c45349b28fefb86511ad323da5e1732c23f52b5687c4d2db0d1aada3</citedby><cites>FETCH-LOGICAL-c3532-ac6cbf9b9c45349b28fefb86511ad323da5e1732c23f52b5687c4d2db0d1aada3</cites><orcidid>0000-0001-7644-8969</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.12692$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.12692$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28544047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Portilho, MM</creatorcontrib><creatorcontrib>Mendonça, ACF</creatorcontrib><creatorcontrib>Marques, VA</creatorcontrib><creatorcontrib>Nabuco, LC</creatorcontrib><creatorcontrib>Villela‐Nogueira, CA</creatorcontrib><creatorcontrib>Ivantes, CAP</creatorcontrib><creatorcontrib>Lewis‐Ximenez, LL</creatorcontrib><creatorcontrib>Lampe, E</creatorcontrib><creatorcontrib>Villar, LM</creatorcontrib><title>Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Background
This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA‐Sal) to detect HBV DNA by qualitative PCR.
Materials and Methods
Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum.
Results
HBV DNA was detected in all serum samples from HBV‐infected individuals, and it was not detected in control group. HBV DNA from HBV group was detected in 17 samples collected with Salivette device, 16 samples collected by FTA Card device, 16 samples collected from spitting and 13 samples collected by DNA‐Sal device. Samples that corresponded to a higher viral load in their paired serum sample could be detected using all oral fluid collection methods, but Salivette collection device yielded the largest numbers of positive samples and had a wide range of viral load that was detected.
Conclusion
It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Dentistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - blood</subject><subject>DNA, Viral - isolation & purification</subject><subject>Female</subject><subject>Fish</subject><subject>Hepatitis B</subject><subject>Hepatitis B - blood</subject><subject>Hepatitis B - diagnosis</subject><subject>Hepatitis B virus - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>molecular detection</subject><subject>oral fluid</subject><subject>Polymerase chain reaction</subject><subject>Saliva - chemistry</subject><subject>Saliva - virology</subject><subject>Specimen Handling - methods</subject><subject>Viral Load</subject><subject>Young Adult</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10ElLAzEYBuAgivvBPyABL3qYmnWWo9YVCr0oeBBCJguNZJqazCj996a2ehDMJYHvycvHC8AJRiOcz2XQboRJ2ZAtsI9LhAtUE76d35SzghP6sgcOUnpDCFcNJbtgj9ScMcSqffA6Dt1CRpfCHAYLQ5QeWj84DVXw3qje5UFn-lnQCdoQYT8zsAt5MngZoTb9xuTPM7OQvetdgtfww8UhHYEdK30yx5v7EDzf3T6NH4rJ9P5xfDUpFOWUFFKVqrVN2yjGKWtaUltj27rkGEtNCdWSG1xRogi1nLS8rCvFNNEt0lhKLekhOF_nLmJ4H0zqReeSMt7LuQlDErhBFJclpizTsz_0LQxxnrfLijW8rlm1UhdrpWJIKRorFtF1Mi4FRmJVuciVi-_Ksz3dJA5tZ_Sv_Ok4g8s1-HTeLP9PEtObx3XkF005iy4</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Portilho, MM</creator><creator>Mendonça, ACF</creator><creator>Marques, VA</creator><creator>Nabuco, LC</creator><creator>Villela‐Nogueira, CA</creator><creator>Ivantes, CAP</creator><creator>Lewis‐Ximenez, LL</creator><creator>Lampe, E</creator><creator>Villar, LM</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7644-8969</orcidid></search><sort><creationdate>201711</creationdate><title>Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus</title><author>Portilho, MM ; Mendonça, ACF ; Marques, VA ; Nabuco, LC ; Villela‐Nogueira, CA ; Ivantes, CAP ; Lewis‐Ximenez, LL ; Lampe, E ; Villar, LM</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-ac6cbf9b9c45349b28fefb86511ad323da5e1732c23f52b5687c4d2db0d1aada3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Dentistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - blood</topic><topic>DNA, Viral - isolation & purification</topic><topic>Female</topic><topic>Fish</topic><topic>Hepatitis B</topic><topic>Hepatitis B - blood</topic><topic>Hepatitis B - diagnosis</topic><topic>Hepatitis B virus - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>molecular detection</topic><topic>oral fluid</topic><topic>Polymerase chain reaction</topic><topic>Saliva - chemistry</topic><topic>Saliva - virology</topic><topic>Specimen Handling - methods</topic><topic>Viral Load</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Portilho, MM</creatorcontrib><creatorcontrib>Mendonça, ACF</creatorcontrib><creatorcontrib>Marques, VA</creatorcontrib><creatorcontrib>Nabuco, LC</creatorcontrib><creatorcontrib>Villela‐Nogueira, CA</creatorcontrib><creatorcontrib>Ivantes, CAP</creatorcontrib><creatorcontrib>Lewis‐Ximenez, LL</creatorcontrib><creatorcontrib>Lampe, E</creatorcontrib><creatorcontrib>Villar, LM</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Portilho, MM</au><au>Mendonça, ACF</au><au>Marques, VA</au><au>Nabuco, LC</au><au>Villela‐Nogueira, CA</au><au>Ivantes, CAP</au><au>Lewis‐Ximenez, LL</au><au>Lampe, E</au><au>Villar, LM</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2017-11</date><risdate>2017</risdate><volume>23</volume><issue>8</issue><spage>1072</spage><epage>1079</epage><pages>1072-1079</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Background
This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA‐Sal) to detect HBV DNA by qualitative PCR.
Materials and Methods
Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum.
Results
HBV DNA was detected in all serum samples from HBV‐infected individuals, and it was not detected in control group. HBV DNA from HBV group was detected in 17 samples collected with Salivette device, 16 samples collected by FTA Card device, 16 samples collected from spitting and 13 samples collected by DNA‐Sal device. Samples that corresponded to a higher viral load in their paired serum sample could be detected using all oral fluid collection methods, but Salivette collection device yielded the largest numbers of positive samples and had a wide range of viral load that was detected.
Conclusion
It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28544047</pmid><doi>10.1111/odi.12692</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7644-8969</orcidid></addata></record> |
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| ispartof | Oral diseases, 2017-11, Vol.23 (8), p.1072-1079 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adolescent Adult Aged Dentistry Deoxyribonucleic acid DNA DNA, Viral - analysis DNA, Viral - blood DNA, Viral - isolation & purification Female Fish Hepatitis B Hepatitis B - blood Hepatitis B - diagnosis Hepatitis B virus - genetics Humans Male Middle Aged molecular detection oral fluid Polymerase chain reaction Saliva - chemistry Saliva - virology Specimen Handling - methods Viral Load Young Adult |
| title | Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus |
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