Engineering muscle cell alignment through 3D bioprinting
Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro cul...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2017-09, Vol.105 (9), p.2582-2588 |
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| container_issue | 9 |
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| container_title | Journal of biomedical materials research. Part A |
| container_volume | 105 |
| creator | Mozetic, Pamela Giannitelli, Sara Maria Gori, Manuele Trombetta, Marcella Rainer, Alberto |
| description | Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell‐seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment. Pluronic/alginate blends have been investigated as a model system for the processing of C2C12 murine myoblast cell line. Interestingly, C2C12 cell model demonstrated cell alignment along the deposition direction, potentially representing a new avenue to tailor the resulting cell histoarchitecture during AM process. Furthermore, the fabricated constructs exhibited high cell viability, as well as a significantly improved expression of myogenic genes vs. conventional 2D cultures. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2582–2588, 2017. |
| doi_str_mv | 10.1002/jbm.a.36117 |
| format | Article |
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Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell‐seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment. Pluronic/alginate blends have been investigated as a model system for the processing of C2C12 murine myoblast cell line. Interestingly, C2C12 cell model demonstrated cell alignment along the deposition direction, potentially representing a new avenue to tailor the resulting cell histoarchitecture during AM process. Furthermore, the fabricated constructs exhibited high cell viability, as well as a significantly improved expression of myogenic genes vs. conventional 2D cultures. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2582–2588, 2017.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.36117</identifier><identifier>PMID: 28544472</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alginic acid ; Alignment ; Animals ; Bioprinting ; Block copolymers ; cell alignment ; Cell culture ; Cell density ; Cell Line ; Cell Proliferation - drug effects ; cell‐laden hydrogel bioprinting ; Fluorescence ; Gene expression ; Gene Expression Regulation - drug effects ; Hydrogels ; In vitro methods and tests ; Manufacturing ; Mice ; Muscle Development - drug effects ; Myoblasts - cytology ; Myoblasts - drug effects ; Printing, Three-Dimensional ; Scaffolds ; skeletal muscle regeneration ; Structure-function relationships ; Three dimensional printing ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell‐seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment. Pluronic/alginate blends have been investigated as a model system for the processing of C2C12 murine myoblast cell line. Interestingly, C2C12 cell model demonstrated cell alignment along the deposition direction, potentially representing a new avenue to tailor the resulting cell histoarchitecture during AM process. Furthermore, the fabricated constructs exhibited high cell viability, as well as a significantly improved expression of myogenic genes vs. conventional 2D cultures. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2582–2588, 2017.</description><subject>Alginic acid</subject><subject>Alignment</subject><subject>Animals</subject><subject>Bioprinting</subject><subject>Block copolymers</subject><subject>cell alignment</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>cell‐laden hydrogel bioprinting</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Hydrogels</subject><subject>In vitro methods and tests</subject><subject>Manufacturing</subject><subject>Mice</subject><subject>Muscle Development - drug effects</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - drug effects</subject><subject>Printing, Three-Dimensional</subject><subject>Scaffolds</subject><subject>skeletal muscle regeneration</subject><subject>Structure-function relationships</subject><subject>Three dimensional printing</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90LtPwzAQBnALgWgpTOwoEgsSSvErdjyWUl4qYoHZcpJzmyqPEidC_e9xSGFgYLobfvp09yF0TvCUYExvNkk5NVMmCJEHaEyiiIZcieiw37kKGVVihE6c23gscESP0YjGEedc0jGKF9UqrwCavFoFZefSAoIUiiIwRb6qSqjaoF03dbdaB-wuSPJ662Xr8Sk6sqZwcLafE_R-v3ibP4bL14en-WwZpkxJGYqYYUYs0FhiJg1LMogFy0SGDROKijihmYUMJCFWEKtoIrhkGCzH1hIQbIKuhtxtU3904Fpd5q6_0FRQd04T5fMF9f94evmHbuquqfx1XlFGJeWqV9eDSpvauQas9i-VptlpgnVfqPaFaqO_C_X6Yp_ZJSVkv_anQQ_oAD7zAnb_Zenn25fZkPoFbEl-zQ</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Mozetic, Pamela</creator><creator>Giannitelli, Sara Maria</creator><creator>Gori, Manuele</creator><creator>Trombetta, Marcella</creator><creator>Rainer, Alberto</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>Engineering muscle cell alignment through 3D bioprinting</title><author>Mozetic, Pamela ; 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Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell‐seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic®) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment. Pluronic/alginate blends have been investigated as a model system for the processing of C2C12 murine myoblast cell line. Interestingly, C2C12 cell model demonstrated cell alignment along the deposition direction, potentially representing a new avenue to tailor the resulting cell histoarchitecture during AM process. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alginic acid Alignment Animals Bioprinting Block copolymers cell alignment Cell culture Cell density Cell Line Cell Proliferation - drug effects cell‐laden hydrogel bioprinting Fluorescence Gene expression Gene Expression Regulation - drug effects Hydrogels In vitro methods and tests Manufacturing Mice Muscle Development - drug effects Myoblasts - cytology Myoblasts - drug effects Printing, Three-Dimensional Scaffolds skeletal muscle regeneration Structure-function relationships Three dimensional printing Tissue engineering Tissue Engineering - methods |
| title | Engineering muscle cell alignment through 3D bioprinting |
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