Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver
Mycobacterium avium subsp. paratuberculosis ( MAP ) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the prese...
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| Veröffentlicht in: | Tropical animal health and production 2017-08, Vol.49 (6), p.1195-1200 |
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| creator | Zarei, Mehdi Ghorbanpour, Masoud Tajbakhsh, Samaneh Mosavari, Nader |
| description | Mycobacterium avium
subsp.
paratuberculosis
(
MAP
) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of
MAP
in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of
MAP
in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the
MAP
antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that
MAP
can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with
MAP
infection in liver. These findings show that the presence of
MAP
in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to
MAP
. Given concerns about a potential zoonotic role for
MAP
, these results show the necessity to find new methods for detecting cattle with
MAP
disseminated infection. |
| doi_str_mv | 10.1007/s11250-017-1317-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1903160561</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1903160561</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-f2c2d435e87bd87021405da03b177cf73d542a8a4752c2bddeb1d67c72afaca13</originalsourceid><addsrcrecordid>eNp1kUuLFDEURoMoTs_oD3AjATduarx5VaqX0vgYGHGj65BXDRmqKmVuqmGW_nPT9CgiuLlZ5HzfTTiEvGJwzQD0O2SMK-iA6Y6JNtQTsmNKi05LMTwlOwC576SW-oJcIt4DtNTQPycXfFAK1F7uyM9DnldbEuaF5pEWu6ZAQ7J3S8aaPK0RK9KaaYg1-kq_PPjsrK-xpG2m9niauDlcr2mrsXVzsfhtypiw1SDGOS22xkDTMrZ8amvSQl0-piXSKR1jeUGejXbC-PLxvCLfP374dvjc3X79dHN4f9t5oXntRu55kELFQbswaOBMggoWhGNa-1GLoCS3g5VaNdKFEB0Lvfaa29F6y8QVeXvuXUv-sbVvmTmhj9Nkl5g3NGwPgvWg-hP65h_0Pm9laa9rFIcexDDwRrEz5UtGLHE0a0mzLQ-GgTn5MWc_pvkxJz9Gtczrx-bNzTH8SfwW0gB-BrBdLXex_LX6v62_ABr1nYQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920603882</pqid></control><display><type>article</type><title>Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Zarei, Mehdi ; Ghorbanpour, Masoud ; Tajbakhsh, Samaneh ; Mosavari, Nader</creator><creatorcontrib>Zarei, Mehdi ; Ghorbanpour, Masoud ; Tajbakhsh, Samaneh ; Mosavari, Nader</creatorcontrib><description>Mycobacterium avium
subsp.
paratuberculosis
(
MAP
) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of
MAP
in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of
MAP
in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the
MAP
antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that
MAP
can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with
MAP
infection in liver. These findings show that the presence of
MAP
in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to
MAP
. Given concerns about a potential zoonotic role for
MAP
, these results show the necessity to find new methods for detecting cattle with
MAP
disseminated infection.</description><identifier>ISSN: 0049-4747</identifier><identifier>EISSN: 1573-7438</identifier><identifier>DOI: 10.1007/s11250-017-1317-5</identifier><identifier>PMID: 28550594</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abattoirs ; Animals ; Beef cattle ; Biomedical and Life Sciences ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; Diagnostic systems ; Diagnostic tests ; Diagnostic Tests, Routine - instrumentation ; Disease control ; Disseminated infection ; Enteritis ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Infections ; Intestine ; Life Sciences ; Liver ; Liver - microbiology ; Lymph nodes ; Meat ; Milk ; Mycobacterium avium subsp. paratuberculosis - isolation & purification ; Paratuberculosis ; Paratuberculosis - diagnosis ; Paratuberculosis - microbiology ; Pathogens ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Public health ; Rectum ; Regular Articles ; Scraping ; Staining ; Veterinary Medicine/Veterinary Science ; Zoology ; Zoonoses</subject><ispartof>Tropical animal health and production, 2017-08, Vol.49 (6), p.1195-1200</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Tropical Animal Health and Production is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-f2c2d435e87bd87021405da03b177cf73d542a8a4752c2bddeb1d67c72afaca13</citedby><cites>FETCH-LOGICAL-c372t-f2c2d435e87bd87021405da03b177cf73d542a8a4752c2bddeb1d67c72afaca13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11250-017-1317-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11250-017-1317-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28550594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zarei, Mehdi</creatorcontrib><creatorcontrib>Ghorbanpour, Masoud</creatorcontrib><creatorcontrib>Tajbakhsh, Samaneh</creatorcontrib><creatorcontrib>Mosavari, Nader</creatorcontrib><title>Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver</title><title>Tropical animal health and production</title><addtitle>Trop Anim Health Prod</addtitle><addtitle>Trop Anim Health Prod</addtitle><description>Mycobacterium avium
subsp.
paratuberculosis
(
MAP
) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of
MAP
in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of
MAP
in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the
MAP
antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that
MAP
can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with
MAP
infection in liver. These findings show that the presence of
MAP
in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to
MAP
. Given concerns about a potential zoonotic role for
MAP
, these results show the necessity to find new methods for detecting cattle with
MAP
disseminated infection.</description><subject>Abattoirs</subject><subject>Animals</subject><subject>Beef cattle</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - microbiology</subject><subject>Diagnostic systems</subject><subject>Diagnostic tests</subject><subject>Diagnostic Tests, Routine - instrumentation</subject><subject>Disease control</subject><subject>Disseminated infection</subject><subject>Enteritis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>Infections</subject><subject>Intestine</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Liver - microbiology</subject><subject>Lymph nodes</subject><subject>Meat</subject><subject>Milk</subject><subject>Mycobacterium avium subsp. paratuberculosis - 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Academic</collection><jtitle>Tropical animal health and production</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zarei, Mehdi</au><au>Ghorbanpour, Masoud</au><au>Tajbakhsh, Samaneh</au><au>Mosavari, Nader</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver</atitle><jtitle>Tropical animal health and production</jtitle><stitle>Trop Anim Health Prod</stitle><addtitle>Trop Anim Health Prod</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>49</volume><issue>6</issue><spage>1195</spage><epage>1200</epage><pages>1195-1200</pages><issn>0049-4747</issn><eissn>1573-7438</eissn><abstract>Mycobacterium avium
subsp.
paratuberculosis
(
MAP
) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of
MAP
in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of
MAP
in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the
MAP
antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that
MAP
can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with
MAP
infection in liver. These findings show that the presence of
MAP
in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to
MAP
. Given concerns about a potential zoonotic role for
MAP
, these results show the necessity to find new methods for detecting cattle with
MAP
disseminated infection.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28550594</pmid><doi>10.1007/s11250-017-1317-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Tropical animal health and production, 2017-08, Vol.49 (6), p.1195-1200 |
| issn | 0049-4747 1573-7438 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Abattoirs Animals Beef cattle Biomedical and Life Sciences Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology Diagnostic systems Diagnostic tests Diagnostic Tests, Routine - instrumentation Disease control Disseminated infection Enteritis Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Female Infections Intestine Life Sciences Liver Liver - microbiology Lymph nodes Meat Milk Mycobacterium avium subsp. paratuberculosis - isolation & purification Paratuberculosis Paratuberculosis - diagnosis Paratuberculosis - microbiology Pathogens Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Public health Rectum Regular Articles Scraping Staining Veterinary Medicine/Veterinary Science Zoology Zoonoses |
| title | Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver |
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