Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis
Animal Science Department, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia 1 Author for correspondence: J. D. Brooker. Tel: +61 8 8303 7357. Fax: +61 8 8303 7114. e-mail: john.brooker{at}adelaide.edu.au Streptococcus gallolyticus ( S. caprinus ) was resistant in vitro to at...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2001-04, Vol.147 (4), p.1025-1033 |
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| creator | O'Donovan, L Brooker, J. D |
| description | Animal Science Department, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia 1
Author for correspondence: J. D. Brooker. Tel: +61 8 8303 7357. Fax: +61 8 8303 7114. e-mail: john.brooker{at}adelaide.edu.au
Streptococcus gallolyticus ( S. caprinus ) was resistant in vitro to at least 7% (w/v) tannic acid and 4% (w/v) acacia condensed tannin, levels 10-fold greater than those tolerated by S. bovis . Growth of S. gallolyticus in liquid medium was characterized by a lag period which increased, and a growth rate which decreased, with increasing tannin concentration. S. gallolyticus was also more tolerant to the presence of simple phenolic acid monomers than was S. bovis , but the lag period was still concentration dependent. Gallate decarboxylase activity in S. gallolyticus was elevated in the presence of tannic acid or gallic acid but not with other phenolic acids. Scanning electron microscopic analysis showed that both the size and shape of S. gallolyticus and S. bovis changed in response to tannin but only S. gallolyticus was surrounded by an extracellular polysaccharide matrix which accumulated in a tannin-concentration-dependent fashion. Washing of the cells to remove extracellular polysaccharide increased the lag period of S. gallolyticus in the presence of 1% (w/v) tannic acid from 4 h to 6 h. In contrast, increasing extracellular polysaccharide synthesis in S. bovis did not increase its tolerance to tannic acid. These data demonstrate that S. gallolyticus has developed a number of mechanisms to reduce the potential effect of tannins on cell growth, and that these mechanisms provide the organism with a selective advantage over S. bovis when grown in the presence of tannins.
Keywords: Streptococcus caprinus , hydrolysable tannins, extracellular polysaccharide matrix, tannin resistance, livestock Abbreviations: CT, condensed tannin; ACT, acacia condensed tannin; EM, extracellular matrix; EPS, extracellular polysaccharide; mBHI, modified brain heart infusion; TA, tannic acid
a Present address: Department of Pathology, University of Tasmania, Hobart, Tas 7000, Australia. |
| doi_str_mv | 10.1099/00221287-147-4-1025 |
| format | Article |
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Author for correspondence: J. D. Brooker. Tel: +61 8 8303 7357. Fax: +61 8 8303 7114. e-mail: john.brooker{at}adelaide.edu.au
Streptococcus gallolyticus ( S. caprinus ) was resistant in vitro to at least 7% (w/v) tannic acid and 4% (w/v) acacia condensed tannin, levels 10-fold greater than those tolerated by S. bovis . Growth of S. gallolyticus in liquid medium was characterized by a lag period which increased, and a growth rate which decreased, with increasing tannin concentration. S. gallolyticus was also more tolerant to the presence of simple phenolic acid monomers than was S. bovis , but the lag period was still concentration dependent. Gallate decarboxylase activity in S. gallolyticus was elevated in the presence of tannic acid or gallic acid but not with other phenolic acids. Scanning electron microscopic analysis showed that both the size and shape of S. gallolyticus and S. bovis changed in response to tannin but only S. gallolyticus was surrounded by an extracellular polysaccharide matrix which accumulated in a tannin-concentration-dependent fashion. Washing of the cells to remove extracellular polysaccharide increased the lag period of S. gallolyticus in the presence of 1% (w/v) tannic acid from 4 h to 6 h. In contrast, increasing extracellular polysaccharide synthesis in S. bovis did not increase its tolerance to tannic acid. These data demonstrate that S. gallolyticus has developed a number of mechanisms to reduce the potential effect of tannins on cell growth, and that these mechanisms provide the organism with a selective advantage over S. bovis when grown in the presence of tannins.
Keywords: Streptococcus caprinus , hydrolysable tannins, extracellular polysaccharide matrix, tannin resistance, livestock Abbreviations: CT, condensed tannin; ACT, acacia condensed tannin; EM, extracellular matrix; EPS, extracellular polysaccharide; mBHI, modified brain heart infusion; TA, tannic acid
a Present address: Department of Pathology, University of Tasmania, Hobart, Tas 7000, Australia.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/00221287-147-4-1025</identifier><identifier>PMID: 11283298</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animal, plant and microbial ecology ; Bacteriology ; Biological and medical sciences ; Carboxy-Lyases - metabolism ; Cell Division ; Culture Media ; Extracellular Matrix - metabolism ; Fundamental and applied biological sciences. Psychology ; Gallic acid ; Gallic Acid - metabolism ; Hydrolysis ; Hydrolyzable Tannins - metabolism ; Metabolism. Enzymes ; Microbial ecology ; Microbiology ; Microscopy, Electron, Scanning ; Normal microflora of man and animals. Rumen ; Polysaccharides, Bacterial - biosynthesis ; Streptococcus - growth & development ; Streptococcus - metabolism ; Streptococcus - ultrastructure ; Streptococcus bovis ; Streptococcus bovis - growth & development ; Streptococcus bovis - metabolism ; Streptococcus bovis - ultrastructure ; Streptococcus gallolyticus ; Tanin</subject><ispartof>Microbiology (Society for General Microbiology), 2001-04, Vol.147 (4), p.1025-1033</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-9411d6c836c259151d1fa6f129c3772b5c90d7eeb7e403f62eacb387793dc6633</citedby><cites>FETCH-LOGICAL-c442t-9411d6c836c259151d1fa6f129c3772b5c90d7eeb7e403f62eacb387793dc6633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1038042$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11283298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Donovan, L</creatorcontrib><creatorcontrib>Brooker, J. D</creatorcontrib><title>Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Animal Science Department, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia 1
Author for correspondence: J. D. Brooker. Tel: +61 8 8303 7357. Fax: +61 8 8303 7114. e-mail: john.brooker{at}adelaide.edu.au
Streptococcus gallolyticus ( S. caprinus ) was resistant in vitro to at least 7% (w/v) tannic acid and 4% (w/v) acacia condensed tannin, levels 10-fold greater than those tolerated by S. bovis . Growth of S. gallolyticus in liquid medium was characterized by a lag period which increased, and a growth rate which decreased, with increasing tannin concentration. S. gallolyticus was also more tolerant to the presence of simple phenolic acid monomers than was S. bovis , but the lag period was still concentration dependent. Gallate decarboxylase activity in S. gallolyticus was elevated in the presence of tannic acid or gallic acid but not with other phenolic acids. Scanning electron microscopic analysis showed that both the size and shape of S. gallolyticus and S. bovis changed in response to tannin but only S. gallolyticus was surrounded by an extracellular polysaccharide matrix which accumulated in a tannin-concentration-dependent fashion. Washing of the cells to remove extracellular polysaccharide increased the lag period of S. gallolyticus in the presence of 1% (w/v) tannic acid from 4 h to 6 h. In contrast, increasing extracellular polysaccharide synthesis in S. bovis did not increase its tolerance to tannic acid. These data demonstrate that S. gallolyticus has developed a number of mechanisms to reduce the potential effect of tannins on cell growth, and that these mechanisms provide the organism with a selective advantage over S. bovis when grown in the presence of tannins.
Keywords: Streptococcus caprinus , hydrolysable tannins, extracellular polysaccharide matrix, tannin resistance, livestock Abbreviations: CT, condensed tannin; ACT, acacia condensed tannin; EM, extracellular matrix; EPS, extracellular polysaccharide; mBHI, modified brain heart infusion; TA, tannic acid
a Present address: Department of Pathology, University of Tasmania, Hobart, Tas 7000, Australia.</description><subject>Animal, plant and microbial ecology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carboxy-Lyases - metabolism</subject><subject>Cell Division</subject><subject>Culture Media</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gallic acid</subject><subject>Gallic Acid - metabolism</subject><subject>Hydrolysis</subject><subject>Hydrolyzable Tannins - metabolism</subject><subject>Metabolism. Enzymes</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Normal microflora of man and animals. Rumen</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Streptococcus - growth & development</subject><subject>Streptococcus - metabolism</subject><subject>Streptococcus - ultrastructure</subject><subject>Streptococcus bovis</subject><subject>Streptococcus bovis - growth & development</subject><subject>Streptococcus bovis - metabolism</subject><subject>Streptococcus bovis - ultrastructure</subject><subject>Streptococcus gallolyticus</subject><subject>Tanin</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kclu1TAUhiMEogM8ARLyAqFWIsVT4niJqjJIlVgU1pbjITFy7IvtUN0H4X3rOyDKhpWP5e__z_H5m-YVglcIcv4eQowRHliLKGtpiyDunjSniPZdi-EAn9aadLCFA8MnzVnOPyCsjxA9b05Q1RHMh9Pm9421RhUQLZi3OkW_zXL0BsiggYpBm5CNBkWG4EIGMYApxfsyvwNLTJs5-jht9-xiihyjd3nZWd2VZDYlqqjUmsEkva_Gxe0uF3dXQMlNcmHNl3vpv_AYf7n8onlmpc_m5fE8b75_vPl2_bm9_frpy_WH21ZRikvLKUK6VwPpFe446pBGVvYWYa4IY3jsFIeaGTMyQyGxPTZSjWRgjBOt-p6Q8-bi4LtJ8edqchGLy8p4L4OJaxaIQ7zfFavo2_-jAyWcI1hBcgBVijknY0X97CLTViAodsGJP8GJGpygYhdcVb0-2q_jYvRfzTGpCrw5AjIr6W2SQbn8yJsMkOKKXR6w2U3zvUtGTCYsrs4yulhHVo96PgDV-LBp</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>O'Donovan, L</creator><creator>Brooker, J. D</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20010401</creationdate><title>Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis</title><author>O'Donovan, L ; Brooker, J. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-9411d6c836c259151d1fa6f129c3772b5c90d7eeb7e403f62eacb387793dc6633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carboxy-Lyases - metabolism</topic><topic>Cell Division</topic><topic>Culture Media</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gallic acid</topic><topic>Gallic Acid - metabolism</topic><topic>Hydrolysis</topic><topic>Hydrolyzable Tannins - metabolism</topic><topic>Metabolism. Enzymes</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Normal microflora of man and animals. Rumen</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Streptococcus - growth & development</topic><topic>Streptococcus - metabolism</topic><topic>Streptococcus - ultrastructure</topic><topic>Streptococcus bovis</topic><topic>Streptococcus bovis - growth & development</topic><topic>Streptococcus bovis - metabolism</topic><topic>Streptococcus bovis - ultrastructure</topic><topic>Streptococcus gallolyticus</topic><topic>Tanin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Donovan, L</creatorcontrib><creatorcontrib>Brooker, J. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Donovan, L</au><au>Brooker, J. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>147</volume><issue>4</issue><spage>1025</spage><epage>1033</epage><pages>1025-1033</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Animal Science Department, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia 1
Author for correspondence: J. D. Brooker. Tel: +61 8 8303 7357. Fax: +61 8 8303 7114. e-mail: john.brooker{at}adelaide.edu.au
Streptococcus gallolyticus ( S. caprinus ) was resistant in vitro to at least 7% (w/v) tannic acid and 4% (w/v) acacia condensed tannin, levels 10-fold greater than those tolerated by S. bovis . Growth of S. gallolyticus in liquid medium was characterized by a lag period which increased, and a growth rate which decreased, with increasing tannin concentration. S. gallolyticus was also more tolerant to the presence of simple phenolic acid monomers than was S. bovis , but the lag period was still concentration dependent. Gallate decarboxylase activity in S. gallolyticus was elevated in the presence of tannic acid or gallic acid but not with other phenolic acids. Scanning electron microscopic analysis showed that both the size and shape of S. gallolyticus and S. bovis changed in response to tannin but only S. gallolyticus was surrounded by an extracellular polysaccharide matrix which accumulated in a tannin-concentration-dependent fashion. Washing of the cells to remove extracellular polysaccharide increased the lag period of S. gallolyticus in the presence of 1% (w/v) tannic acid from 4 h to 6 h. In contrast, increasing extracellular polysaccharide synthesis in S. bovis did not increase its tolerance to tannic acid. These data demonstrate that S. gallolyticus has developed a number of mechanisms to reduce the potential effect of tannins on cell growth, and that these mechanisms provide the organism with a selective advantage over S. bovis when grown in the presence of tannins.
Keywords: Streptococcus caprinus , hydrolysable tannins, extracellular polysaccharide matrix, tannin resistance, livestock Abbreviations: CT, condensed tannin; ACT, acacia condensed tannin; EM, extracellular matrix; EPS, extracellular polysaccharide; mBHI, modified brain heart infusion; TA, tannic acid
a Present address: Department of Pathology, University of Tasmania, Hobart, Tas 7000, Australia.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>11283298</pmid><doi>10.1099/00221287-147-4-1025</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal, plant and microbial ecology Bacteriology Biological and medical sciences Carboxy-Lyases - metabolism Cell Division Culture Media Extracellular Matrix - metabolism Fundamental and applied biological sciences. Psychology Gallic acid Gallic Acid - metabolism Hydrolysis Hydrolyzable Tannins - metabolism Metabolism. Enzymes Microbial ecology Microbiology Microscopy, Electron, Scanning Normal microflora of man and animals. Rumen Polysaccharides, Bacterial - biosynthesis Streptococcus - growth & development Streptococcus - metabolism Streptococcus - ultrastructure Streptococcus bovis Streptococcus bovis - growth & development Streptococcus bovis - metabolism Streptococcus bovis - ultrastructure Streptococcus gallolyticus Tanin |
| title | Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis |
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