Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners
Recently, it has been disclosed that silver nanoparticles (AgNPs) have the potential to inhibit infection and cancerous cells and eventually penetrate through injected site into the capillary due to their small size. This study focuses on the effect of size and zeta potential of bare and citrate-coa...
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| description | Recently, it has been disclosed that silver nanoparticles (AgNPs) have the potential to inhibit infection and cancerous cells and eventually penetrate through injected site into the capillary due to their small size. This study focuses on the effect of size and zeta potential of bare and citrate-coated AgNPs on human umbilical vein endothelial cells (HUVECs) as main capillary cells. AgNPs with high and low concentrations and no citrate coating were synthesized by using simple wet chemical method and named as AgNP/HC, AgNP/LC, and AgNP, respectively. Citrate coated particles showed larger zeta potential of −22 mV and AgNp/HC showed the smallest size of 13.2 nm. UV-Visible spectroscopy and dynamic light scattering (DLS) were performed to evaluate particle size and hydrodynamic diameter of NPs in water and cell culture media. Results indicated that higher concentrations of citrate decreased hydrodynamic diameter and NP agglomeration. reactive oxygen species (ROS) production of all AgNPs was similar at 28 ppm although it was significantly higher than control group. Their effects on cell membrane and chromosomal structure were studied using LDH measurement and 4′,6-diamidino-2-phenylindole (DAPI) staining, as well. Results demonstrated that AgNP/LC was less toxic to cells owing to higher value of IC
50
, minimum inhibitory concentration (MIC), and less release of LDH. Cancerous (Human Caucasian neuroblastoma) and immortal cells (Mouse embryonic fibroblast cell line) were about twice more sensitive than HUVECs to toxic effects of AgNPs. DAPI staining results showed that AgNP and AgNP/HC induced highest and lowest breaking of chromosome. Overall results suggest that viability of HUVECs will be higher than 90% when viability of cancerous cells is 50% in AgNPs chemotherapy. |
| doi_str_mv | 10.1080/21691401.2016.1178132 |
| format | Article |
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50
, minimum inhibitory concentration (MIC), and less release of LDH. Cancerous (Human Caucasian neuroblastoma) and immortal cells (Mouse embryonic fibroblast cell line) were about twice more sensitive than HUVECs to toxic effects of AgNPs. DAPI staining results showed that AgNP and AgNP/HC induced highest and lowest breaking of chromosome. Overall results suggest that viability of HUVECs will be higher than 90% when viability of cancerous cells is 50% in AgNPs chemotherapy.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.1080/21691401.2016.1178132</identifier><identifier>PMID: 27160016</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Cancer ; Cell culture ; Cell membranes ; Cell Survival - drug effects ; Chemical synthesis ; Chemotherapy ; Chromatin ; Chromatin - drug effects ; Chromatin - metabolism ; Chromosomes ; Citrates - chemistry ; Citric acid ; Coated particles ; Coatings ; Culture media ; Embryo fibroblasts ; Endothelial cells ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - pharmacology ; human umbilical vein endothelial cell ; Human Umbilical Vein Endothelial Cells - cytology ; Human Umbilical Vein Endothelial Cells - drug effects ; Human Umbilical Vein Endothelial Cells - secretion ; Humans ; L-Lactate Dehydrogenase - secretion ; Light scattering ; Low concentrations ; Metal Nanoparticles ; Minimum inhibitory concentration ; Nanoparticles ; Neuroblastoma ; Organelles ; Organelles - drug effects ; Organelles - metabolism ; Organic chemistry ; Particle Size ; Photon correlation spectroscopy ; Reactive oxygen species ; Silver ; Silver - chemistry ; Silver - pharmacology ; silver nanoparticle ; Spectroscopy ; Staining ; Umbilical vein ; Veins ; Viability ; Zeta potential</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2017-06, Vol.45 (4), p.817-823</ispartof><rights>2016 Informa UK Limited, trading as Taylor & Francis Group 2016</rights><rights>2016 Informa UK Limited, trading as Taylor & Francis Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-f564176d20cf90ebfb91cca59eafe04acac3bf6b2e9743d120a63d878116eae3</citedby><cites>FETCH-LOGICAL-c474t-f564176d20cf90ebfb91cca59eafe04acac3bf6b2e9743d120a63d878116eae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27160016$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tavakol, Shima</creatorcontrib><creatorcontrib>Hoveizi, Elham</creatorcontrib><creatorcontrib>Kharrazi, Sharmin</creatorcontrib><creatorcontrib>Tavakol, Behnaz</creatorcontrib><creatorcontrib>Karimi, Shabnam</creatorcontrib><creatorcontrib>Rezayat Sorkhabadi, Seyed Mahdi</creatorcontrib><title>Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>Recently, it has been disclosed that silver nanoparticles (AgNPs) have the potential to inhibit infection and cancerous cells and eventually penetrate through injected site into the capillary due to their small size. This study focuses on the effect of size and zeta potential of bare and citrate-coated AgNPs on human umbilical vein endothelial cells (HUVECs) as main capillary cells. AgNPs with high and low concentrations and no citrate coating were synthesized by using simple wet chemical method and named as AgNP/HC, AgNP/LC, and AgNP, respectively. Citrate coated particles showed larger zeta potential of −22 mV and AgNp/HC showed the smallest size of 13.2 nm. UV-Visible spectroscopy and dynamic light scattering (DLS) were performed to evaluate particle size and hydrodynamic diameter of NPs in water and cell culture media. Results indicated that higher concentrations of citrate decreased hydrodynamic diameter and NP agglomeration. reactive oxygen species (ROS) production of all AgNPs was similar at 28 ppm although it was significantly higher than control group. Their effects on cell membrane and chromosomal structure were studied using LDH measurement and 4′,6-diamidino-2-phenylindole (DAPI) staining, as well. Results demonstrated that AgNP/LC was less toxic to cells owing to higher value of IC
50
, minimum inhibitory concentration (MIC), and less release of LDH. Cancerous (Human Caucasian neuroblastoma) and immortal cells (Mouse embryonic fibroblast cell line) were about twice more sensitive than HUVECs to toxic effects of AgNPs. DAPI staining results showed that AgNP and AgNP/HC induced highest and lowest breaking of chromosome. Overall results suggest that viability of HUVECs will be higher than 90% when viability of cancerous cells is 50% in AgNPs chemotherapy.</description><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell membranes</subject><subject>Cell Survival - drug effects</subject><subject>Chemical synthesis</subject><subject>Chemotherapy</subject><subject>Chromatin</subject><subject>Chromatin - drug effects</subject><subject>Chromatin - metabolism</subject><subject>Chromosomes</subject><subject>Citrates - chemistry</subject><subject>Citric acid</subject><subject>Coated particles</subject><subject>Coatings</subject><subject>Culture media</subject><subject>Embryo fibroblasts</subject><subject>Endothelial cells</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>human umbilical vein endothelial cell</subject><subject>Human Umbilical Vein Endothelial Cells - cytology</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Human Umbilical Vein Endothelial Cells - secretion</subject><subject>Humans</subject><subject>L-Lactate Dehydrogenase - secretion</subject><subject>Light scattering</subject><subject>Low concentrations</subject><subject>Metal Nanoparticles</subject><subject>Minimum inhibitory concentration</subject><subject>Nanoparticles</subject><subject>Neuroblastoma</subject><subject>Organelles</subject><subject>Organelles - drug effects</subject><subject>Organelles - metabolism</subject><subject>Organic chemistry</subject><subject>Particle Size</subject><subject>Photon correlation spectroscopy</subject><subject>Reactive oxygen species</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>silver nanoparticle</subject><subject>Spectroscopy</subject><subject>Staining</subject><subject>Umbilical vein</subject><subject>Veins</subject><subject>Viability</subject><subject>Zeta potential</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctuFDEQRVsIRKKQTwBZYsNmBle_eweKeEmRssmCnVXtLmccue3BdgdNvopPpFozyYIF3vh17i27blG8BbkF2cuPJbQD1BK2pYR2C9D1UJUvivP1fAM1_Hz5vJZwVlymdC959NB2Tf26OCs7aCVLz4s_N_EOPTlHSaCfhN7FMGO2XpiIdzP5zJvgRTBit8zoxTKP1lmNTjwQU-SnkHfkLB9othHWG7eQ1yTGg-AbQcaQzml1eKSMYh8yu678Wi_ZR1qvknUPFIVHH_YYs9Xrg9h_siyPLBBc3FNMb4pXBl2iy9N8Udx-_XJ79X1zffPtx9Xn642uuzpvTNPW0LVTKbUZJI1mHEBrbAZCQ7JGjboaTTuWNHR1NUEpsa2mnhsJLSFVF8WHo-0-hl8Lpaxmm9YPcrPCkhQMErqqa6Rk9P0_6H1YoufHKej7su6aYaiYao6UjiGlSEbto50xHhRItYaqnkJVa6jqFCrr3p3cl3Gm6Vn1FCEDn44Adz7EGX-H6CaV8eBC5Ay9tklV_6_xFwOetOc</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Tavakol, Shima</creator><creator>Hoveizi, Elham</creator><creator>Kharrazi, Sharmin</creator><creator>Tavakol, Behnaz</creator><creator>Karimi, Shabnam</creator><creator>Rezayat Sorkhabadi, Seyed Mahdi</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20170601</creationdate><title>Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners</title><author>Tavakol, Shima ; Hoveizi, Elham ; Kharrazi, Sharmin ; Tavakol, Behnaz ; Karimi, Shabnam ; Rezayat Sorkhabadi, Seyed Mahdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-f564176d20cf90ebfb91cca59eafe04acac3bf6b2e9743d120a63d878116eae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Cancer</topic><topic>Cell culture</topic><topic>Cell membranes</topic><topic>Cell Survival - drug effects</topic><topic>Chemical synthesis</topic><topic>Chemotherapy</topic><topic>Chromatin</topic><topic>Chromatin - drug effects</topic><topic>Chromatin - metabolism</topic><topic>Chromosomes</topic><topic>Citrates - chemistry</topic><topic>Citric acid</topic><topic>Coated particles</topic><topic>Coatings</topic><topic>Culture media</topic><topic>Embryo fibroblasts</topic><topic>Endothelial cells</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>human umbilical vein endothelial cell</topic><topic>Human Umbilical Vein Endothelial Cells - cytology</topic><topic>Human Umbilical Vein Endothelial Cells - drug effects</topic><topic>Human Umbilical Vein Endothelial Cells - secretion</topic><topic>Humans</topic><topic>L-Lactate Dehydrogenase - secretion</topic><topic>Light scattering</topic><topic>Low concentrations</topic><topic>Metal Nanoparticles</topic><topic>Minimum inhibitory concentration</topic><topic>Nanoparticles</topic><topic>Neuroblastoma</topic><topic>Organelles</topic><topic>Organelles - drug effects</topic><topic>Organelles - metabolism</topic><topic>Organic chemistry</topic><topic>Particle Size</topic><topic>Photon correlation spectroscopy</topic><topic>Reactive oxygen species</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>silver nanoparticle</topic><topic>Spectroscopy</topic><topic>Staining</topic><topic>Umbilical vein</topic><topic>Veins</topic><topic>Viability</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tavakol, Shima</creatorcontrib><creatorcontrib>Hoveizi, Elham</creatorcontrib><creatorcontrib>Kharrazi, Sharmin</creatorcontrib><creatorcontrib>Tavakol, Behnaz</creatorcontrib><creatorcontrib>Karimi, Shabnam</creatorcontrib><creatorcontrib>Rezayat Sorkhabadi, Seyed Mahdi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tavakol, Shima</au><au>Hoveizi, Elham</au><au>Kharrazi, Sharmin</au><au>Tavakol, Behnaz</au><au>Karimi, Shabnam</au><au>Rezayat Sorkhabadi, Seyed Mahdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2017-06-01</date><risdate>2017</risdate><volume>45</volume><issue>4</issue><spage>817</spage><epage>823</epage><pages>817-823</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Recently, it has been disclosed that silver nanoparticles (AgNPs) have the potential to inhibit infection and cancerous cells and eventually penetrate through injected site into the capillary due to their small size. This study focuses on the effect of size and zeta potential of bare and citrate-coated AgNPs on human umbilical vein endothelial cells (HUVECs) as main capillary cells. AgNPs with high and low concentrations and no citrate coating were synthesized by using simple wet chemical method and named as AgNP/HC, AgNP/LC, and AgNP, respectively. Citrate coated particles showed larger zeta potential of −22 mV and AgNp/HC showed the smallest size of 13.2 nm. UV-Visible spectroscopy and dynamic light scattering (DLS) were performed to evaluate particle size and hydrodynamic diameter of NPs in water and cell culture media. Results indicated that higher concentrations of citrate decreased hydrodynamic diameter and NP agglomeration. reactive oxygen species (ROS) production of all AgNPs was similar at 28 ppm although it was significantly higher than control group. Their effects on cell membrane and chromosomal structure were studied using LDH measurement and 4′,6-diamidino-2-phenylindole (DAPI) staining, as well. Results demonstrated that AgNP/LC was less toxic to cells owing to higher value of IC
50
, minimum inhibitory concentration (MIC), and less release of LDH. Cancerous (Human Caucasian neuroblastoma) and immortal cells (Mouse embryonic fibroblast cell line) were about twice more sensitive than HUVECs to toxic effects of AgNPs. DAPI staining results showed that AgNP and AgNP/HC induced highest and lowest breaking of chromosome. Overall results suggest that viability of HUVECs will be higher than 90% when viability of cancerous cells is 50% in AgNPs chemotherapy.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>27160016</pmid><doi>10.1080/21691401.2016.1178132</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cancer Cell culture Cell membranes Cell Survival - drug effects Chemical synthesis Chemotherapy Chromatin Chromatin - drug effects Chromatin - metabolism Chromosomes Citrates - chemistry Citric acid Coated particles Coatings Culture media Embryo fibroblasts Endothelial cells Free Radical Scavengers - chemistry Free Radical Scavengers - pharmacology human umbilical vein endothelial cell Human Umbilical Vein Endothelial Cells - cytology Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - secretion Humans L-Lactate Dehydrogenase - secretion Light scattering Low concentrations Metal Nanoparticles Minimum inhibitory concentration Nanoparticles Neuroblastoma Organelles Organelles - drug effects Organelles - metabolism Organic chemistry Particle Size Photon correlation spectroscopy Reactive oxygen species Silver Silver - chemistry Silver - pharmacology silver nanoparticle Spectroscopy Staining Umbilical vein Veins Viability Zeta potential |
| title | Organelles and chromatin fragmentation of human umbilical vein endothelial cell influence by the effects of zeta potential and size of silver nanoparticles in different manners |
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