Immuno-PCR, a new technique for the serodiagnosis of tuberculosis
Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection d...
Gespeichert in:
| Veröffentlicht in: | Journal of microbiological methods 2017-08, Vol.139, p.218-229 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 229 |
|---|---|
| container_issue | |
| container_start_page | 218 |
| container_title | Journal of microbiological methods |
| container_volume | 139 |
| creator | Mehta, Promod K. Dahiya, Bhawna Sharma, Suman Singh, Netrapal Dharra, Renu Thakur, Zoozeal Mehta, Neeru Gupta, Krishna B. Gupta, Mahesh C. Chaudhary, Dhruva |
| description | Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection due to inconsistent and imprecise results has stimulated renewed interest in the development of rapid antigen detection methods. However, the World Health Organization (WHO) has emphasized to continue research for designing new antibody-based detection tests with improved accuracy. Immuno-polymerase chain reaction (I-PCR) combines the simplicity and versatility of enzyme-linked immunosorbent assay (ELISA) with the exponential amplification capacity and sensitivity of PCR thus leading to several-fold increase in sensitivity in comparison to analogous ELISA. In this review, we have described the serodiagnostic potential of I-PCR assays for an early diagnosis of TB based on the detection of potential mycobacterial antigens and circulating antibodies in body fluids of TB patients.
•Immuno-PCR (I-PCR) combines ELISA and PCR.•Antigens/antibodies were detected in TB patients by I-PCR.•I-PCR showed better results than ELISA.•I-PCR assay may facilitate an early diagnosis of TB patients. |
| doi_str_mv | 10.1016/j.mimet.2017.05.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1901310197</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0167701217301197</els_id><sourcerecordid>1901310197</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-9192cb41d270e6550b32ea3e57f92e5d9ec2ad40f612080ab54e8396a8227adc3</originalsourceid><addsrcrecordid>eNp9kMtKxDAUhoMoOl6eQJAuXdh6kk6aZOFCBi8DgiK6Dml66mSYNpq0im9vxlGXrg4Hvv9cPkKOKRQUaHW-LDrX4VAwoKIAXgCoLTKhUrBcllxtk0miRC6Asj2yH-MSgPJyKnfJHpOcCSmrCbmcd93Y-_xh9niWmazHj2xAu-jd24hZ60M2LDCLGHzjzEvvo4uZb7NhrDHYcbXuD8lOa1YRj37qAXm-vnqa3eZ39zfz2eVdbtMxQ66oYrae0oYJwIpzqEuGpkQuWsWQNwotM80U2ooykGBqPkVZqspIxoRpbHlATjdzX4NPx8VBdy5aXK1Mj36MmiqgZRKjRELLDWqDjzFgq1-D60z41BT02p1e6m93eu1OA9fJXUqd_CwY6w6bv8yvrARcbABMb747DDpah73FxgW0g268-3fBF891gAk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1901310197</pqid></control><display><type>article</type><title>Immuno-PCR, a new technique for the serodiagnosis of tuberculosis</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Mehta, Promod K. ; Dahiya, Bhawna ; Sharma, Suman ; Singh, Netrapal ; Dharra, Renu ; Thakur, Zoozeal ; Mehta, Neeru ; Gupta, Krishna B. ; Gupta, Mahesh C. ; Chaudhary, Dhruva</creator><creatorcontrib>Mehta, Promod K. ; Dahiya, Bhawna ; Sharma, Suman ; Singh, Netrapal ; Dharra, Renu ; Thakur, Zoozeal ; Mehta, Neeru ; Gupta, Krishna B. ; Gupta, Mahesh C. ; Chaudhary, Dhruva</creatorcontrib><description>Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection due to inconsistent and imprecise results has stimulated renewed interest in the development of rapid antigen detection methods. However, the World Health Organization (WHO) has emphasized to continue research for designing new antibody-based detection tests with improved accuracy. Immuno-polymerase chain reaction (I-PCR) combines the simplicity and versatility of enzyme-linked immunosorbent assay (ELISA) with the exponential amplification capacity and sensitivity of PCR thus leading to several-fold increase in sensitivity in comparison to analogous ELISA. In this review, we have described the serodiagnostic potential of I-PCR assays for an early diagnosis of TB based on the detection of potential mycobacterial antigens and circulating antibodies in body fluids of TB patients.
•Immuno-PCR (I-PCR) combines ELISA and PCR.•Antigens/antibodies were detected in TB patients by I-PCR.•I-PCR showed better results than ELISA.•I-PCR assay may facilitate an early diagnosis of TB patients.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2017.05.009</identifier><identifier>PMID: 28527886</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies - chemistry ; Antibodies, Bacterial - analysis ; Antibody detection ; Antigen detection ; Antigens, Bacterial - analysis ; Antigens, Bacterial - immunology ; DNA, Bacterial ; Enzyme-Linked Immunosorbent Assay - methods ; Humans ; Immuno-PCR ; Mice ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - immunology ; Mycobacterium tuberculosis - isolation & purification ; Point-of-Care Systems ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity ; Serodiagnosis ; Serologic Tests - methods ; Tuberculosis ; Tuberculosis - diagnosis ; Tuberculosis - immunology ; Tuberculosis - microbiology ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - immunology ; Tuberculosis, Pulmonary - microbiology</subject><ispartof>Journal of microbiological methods, 2017-08, Vol.139, p.218-229</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-9192cb41d270e6550b32ea3e57f92e5d9ec2ad40f612080ab54e8396a8227adc3</citedby><cites>FETCH-LOGICAL-c359t-9192cb41d270e6550b32ea3e57f92e5d9ec2ad40f612080ab54e8396a8227adc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2017.05.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28527886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehta, Promod K.</creatorcontrib><creatorcontrib>Dahiya, Bhawna</creatorcontrib><creatorcontrib>Sharma, Suman</creatorcontrib><creatorcontrib>Singh, Netrapal</creatorcontrib><creatorcontrib>Dharra, Renu</creatorcontrib><creatorcontrib>Thakur, Zoozeal</creatorcontrib><creatorcontrib>Mehta, Neeru</creatorcontrib><creatorcontrib>Gupta, Krishna B.</creatorcontrib><creatorcontrib>Gupta, Mahesh C.</creatorcontrib><creatorcontrib>Chaudhary, Dhruva</creatorcontrib><title>Immuno-PCR, a new technique for the serodiagnosis of tuberculosis</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection due to inconsistent and imprecise results has stimulated renewed interest in the development of rapid antigen detection methods. However, the World Health Organization (WHO) has emphasized to continue research for designing new antibody-based detection tests with improved accuracy. Immuno-polymerase chain reaction (I-PCR) combines the simplicity and versatility of enzyme-linked immunosorbent assay (ELISA) with the exponential amplification capacity and sensitivity of PCR thus leading to several-fold increase in sensitivity in comparison to analogous ELISA. In this review, we have described the serodiagnostic potential of I-PCR assays for an early diagnosis of TB based on the detection of potential mycobacterial antigens and circulating antibodies in body fluids of TB patients.
•Immuno-PCR (I-PCR) combines ELISA and PCR.•Antigens/antibodies were detected in TB patients by I-PCR.•I-PCR showed better results than ELISA.•I-PCR assay may facilitate an early diagnosis of TB patients.</description><subject>Animals</subject><subject>Antibodies - chemistry</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Antibody detection</subject><subject>Antigen detection</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Bacterial - immunology</subject><subject>DNA, Bacterial</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Humans</subject><subject>Immuno-PCR</subject><subject>Mice</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - immunology</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Point-of-Care Systems</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><subject>Serodiagnosis</subject><subject>Serologic Tests - methods</subject><subject>Tuberculosis</subject><subject>Tuberculosis - diagnosis</subject><subject>Tuberculosis - immunology</subject><subject>Tuberculosis - microbiology</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - immunology</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMoOl6eQJAuXdh6kk6aZOFCBi8DgiK6Dml66mSYNpq0im9vxlGXrg4Hvv9cPkKOKRQUaHW-LDrX4VAwoKIAXgCoLTKhUrBcllxtk0miRC6Asj2yH-MSgPJyKnfJHpOcCSmrCbmcd93Y-_xh9niWmazHj2xAu-jd24hZ60M2LDCLGHzjzEvvo4uZb7NhrDHYcbXuD8lOa1YRj37qAXm-vnqa3eZ39zfz2eVdbtMxQ66oYrae0oYJwIpzqEuGpkQuWsWQNwotM80U2ooykGBqPkVZqspIxoRpbHlATjdzX4NPx8VBdy5aXK1Mj36MmiqgZRKjRELLDWqDjzFgq1-D60z41BT02p1e6m93eu1OA9fJXUqd_CwY6w6bv8yvrARcbABMb747DDpah73FxgW0g268-3fBF891gAk</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Mehta, Promod K.</creator><creator>Dahiya, Bhawna</creator><creator>Sharma, Suman</creator><creator>Singh, Netrapal</creator><creator>Dharra, Renu</creator><creator>Thakur, Zoozeal</creator><creator>Mehta, Neeru</creator><creator>Gupta, Krishna B.</creator><creator>Gupta, Mahesh C.</creator><creator>Chaudhary, Dhruva</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>Immuno-PCR, a new technique for the serodiagnosis of tuberculosis</title><author>Mehta, Promod K. ; Dahiya, Bhawna ; Sharma, Suman ; Singh, Netrapal ; Dharra, Renu ; Thakur, Zoozeal ; Mehta, Neeru ; Gupta, Krishna B. ; Gupta, Mahesh C. ; Chaudhary, Dhruva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-9192cb41d270e6550b32ea3e57f92e5d9ec2ad40f612080ab54e8396a8227adc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antibodies - chemistry</topic><topic>Antibodies, Bacterial - analysis</topic><topic>Antibody detection</topic><topic>Antigen detection</topic><topic>Antigens, Bacterial - analysis</topic><topic>Antigens, Bacterial - immunology</topic><topic>DNA, Bacterial</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Humans</topic><topic>Immuno-PCR</topic><topic>Mice</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - immunology</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Point-of-Care Systems</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><topic>Serodiagnosis</topic><topic>Serologic Tests - methods</topic><topic>Tuberculosis</topic><topic>Tuberculosis - diagnosis</topic><topic>Tuberculosis - immunology</topic><topic>Tuberculosis - microbiology</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><topic>Tuberculosis, Pulmonary - immunology</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehta, Promod K.</creatorcontrib><creatorcontrib>Dahiya, Bhawna</creatorcontrib><creatorcontrib>Sharma, Suman</creatorcontrib><creatorcontrib>Singh, Netrapal</creatorcontrib><creatorcontrib>Dharra, Renu</creatorcontrib><creatorcontrib>Thakur, Zoozeal</creatorcontrib><creatorcontrib>Mehta, Neeru</creatorcontrib><creatorcontrib>Gupta, Krishna B.</creatorcontrib><creatorcontrib>Gupta, Mahesh C.</creatorcontrib><creatorcontrib>Chaudhary, Dhruva</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehta, Promod K.</au><au>Dahiya, Bhawna</au><au>Sharma, Suman</au><au>Singh, Netrapal</au><au>Dharra, Renu</au><au>Thakur, Zoozeal</au><au>Mehta, Neeru</au><au>Gupta, Krishna B.</au><au>Gupta, Mahesh C.</au><au>Chaudhary, Dhruva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immuno-PCR, a new technique for the serodiagnosis of tuberculosis</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2017-08</date><risdate>2017</risdate><volume>139</volume><spage>218</spage><epage>229</epage><pages>218-229</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection due to inconsistent and imprecise results has stimulated renewed interest in the development of rapid antigen detection methods. However, the World Health Organization (WHO) has emphasized to continue research for designing new antibody-based detection tests with improved accuracy. Immuno-polymerase chain reaction (I-PCR) combines the simplicity and versatility of enzyme-linked immunosorbent assay (ELISA) with the exponential amplification capacity and sensitivity of PCR thus leading to several-fold increase in sensitivity in comparison to analogous ELISA. In this review, we have described the serodiagnostic potential of I-PCR assays for an early diagnosis of TB based on the detection of potential mycobacterial antigens and circulating antibodies in body fluids of TB patients.
•Immuno-PCR (I-PCR) combines ELISA and PCR.•Antigens/antibodies were detected in TB patients by I-PCR.•I-PCR showed better results than ELISA.•I-PCR assay may facilitate an early diagnosis of TB patients.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28527886</pmid><doi>10.1016/j.mimet.2017.05.009</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2017-08, Vol.139, p.218-229 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1901310197 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies - chemistry Antibodies, Bacterial - analysis Antibody detection Antigen detection Antigens, Bacterial - analysis Antigens, Bacterial - immunology DNA, Bacterial Enzyme-Linked Immunosorbent Assay - methods Humans Immuno-PCR Mice Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - immunology Mycobacterium tuberculosis - isolation & purification Point-of-Care Systems Polymerase Chain Reaction - methods Sensitivity and Specificity Serodiagnosis Serologic Tests - methods Tuberculosis Tuberculosis - diagnosis Tuberculosis - immunology Tuberculosis - microbiology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - immunology Tuberculosis, Pulmonary - microbiology |
| title | Immuno-PCR, a new technique for the serodiagnosis of tuberculosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T17%3A47%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immuno-PCR,%20a%20new%20technique%20for%20the%20serodiagnosis%20of%20tuberculosis&rft.jtitle=Journal%20of%20microbiological%20methods&rft.au=Mehta,%20Promod%20K.&rft.date=2017-08&rft.volume=139&rft.spage=218&rft.epage=229&rft.pages=218-229&rft.issn=0167-7012&rft.eissn=1872-8359&rft_id=info:doi/10.1016/j.mimet.2017.05.009&rft_dat=%3Cproquest_cross%3E1901310197%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1901310197&rft_id=info:pmid/28527886&rft_els_id=S0167701217301197&rfr_iscdi=true |