Apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in malignant transformation of salivary gland pleomorphic adenoma
DNA repair systems play a critical role in protecting the human genome against cumulative damage. The apurinic/apyrimidinic endonuclease 1 is a protein involved in DNA base excision repair and its expression still needs to be investigated in salivary gland tumors. The objective of this study is to a...
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| Veröffentlicht in: | European archives of oto-rhino-laryngology 2017-08, Vol.274 (8), p.3203-3209 |
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| creator | Silva, Leorik Pereira Santana, Thalita Sedassari, Bruno Tavares de Sousa, Suzana Machado Sobral, Ana Paula Veras Freitas, Roseana de Almeida Barboza, Carlos Augusto Galvão de Souza, Lélia Batista |
| description | DNA repair systems play a critical role in protecting the human genome against cumulative damage. The apurinic/apyrimidinic endonuclease 1 is a protein involved in DNA base excision repair and its expression still needs to be investigated in salivary gland tumors. The objective of this study is to analyze the immunoexpression of apurinic/apyrimidinic endonuclease 1 in pleomorphic adenomas and carcinomas ex pleomorphic adenomas of the salivary glands. A total of 33 pleomorphic adenomas and 16 carcinomas ex pleomorphic adenomas of the salivary glands underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed quantitatively. For statistical analysis, Mann–Whitney test was performed and a significance level was set at
p
≤ 0.05. All analyzed tumors (
n
= 49) were positive for apurinic/apyrimidinic endonuclease 1. However, there was a higher median expression in carcinomas ex pleomorphic adenomas (
p
|
| doi_str_mv | 10.1007/s00405-017-4605-9 |
| format | Article |
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p
≤ 0.05. All analyzed tumors (
n
= 49) were positive for apurinic/apyrimidinic endonuclease 1. However, there was a higher median expression in carcinomas ex pleomorphic adenomas (
p
< 0.001). There was no difference between this protein immunoexpression and tumors of major or minor salivary gland. Overexpression was found mainly in cases of carcinomas ex pleomorphic adenomas with lymph node metastasis (
p
= 0.002) and invasive growth (
p
= 0.003), when compared to cases without metastasis and without capsular invasion (intracapsular pattern). Our findings revealed that apurinic/apyrimidinic endonuclease 1 is downregulated in pleomorphic adenomas and overexpressed in carcinomas ex pleomorphic adenomas, suggesting that this protein is possibly deregulated in pleomorphic adenoma malignant transformation. Furthermore, the increased expression of this protein is associated with a more aggressive behavior in carcinomas ex pleomorphic adenomas, which suggests that this protein may represent a prognostic biomarker in the studied salivary gland tumors.</description><identifier>ISSN: 0937-4477</identifier><identifier>EISSN: 1434-4726</identifier><identifier>DOI: 10.1007/s00405-017-4605-9</identifier><identifier>PMID: 28523411</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenoma, Pleomorphic - genetics ; Adenoma, Pleomorphic - pathology ; Adult ; Carcinoma - genetics ; Carcinoma - pathology ; Cell Transformation, Neoplastic - genetics ; DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics ; Female ; Gene Expression Profiling ; Head and Neck ; Head and Neck Surgery ; Humans ; Immunohistochemistry ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Neurosurgery ; Otorhinolaryngology ; Predictive Value of Tests ; Proteomics - methods ; Salivary Gland Neoplasms - genetics ; Salivary Gland Neoplasms - pathology ; Salivary Glands - pathology</subject><ispartof>European archives of oto-rhino-laryngology, 2017-08, Vol.274 (8), p.3203-3209</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-a5b5c3cd88c7d120d9557dbc5affffb771dd23716579342b758c9188c4e8b773</citedby><cites>FETCH-LOGICAL-c410t-a5b5c3cd88c7d120d9557dbc5affffb771dd23716579342b758c9188c4e8b773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00405-017-4605-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00405-017-4605-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28523411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Leorik Pereira</creatorcontrib><creatorcontrib>Santana, Thalita</creatorcontrib><creatorcontrib>Sedassari, Bruno Tavares</creatorcontrib><creatorcontrib>de Sousa, Suzana Machado</creatorcontrib><creatorcontrib>Sobral, Ana Paula Veras</creatorcontrib><creatorcontrib>Freitas, Roseana de Almeida</creatorcontrib><creatorcontrib>Barboza, Carlos Augusto Galvão</creatorcontrib><creatorcontrib>de Souza, Lélia Batista</creatorcontrib><title>Apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in malignant transformation of salivary gland pleomorphic adenoma</title><title>European archives of oto-rhino-laryngology</title><addtitle>Eur Arch Otorhinolaryngol</addtitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><description>DNA repair systems play a critical role in protecting the human genome against cumulative damage. The apurinic/apyrimidinic endonuclease 1 is a protein involved in DNA base excision repair and its expression still needs to be investigated in salivary gland tumors. The objective of this study is to analyze the immunoexpression of apurinic/apyrimidinic endonuclease 1 in pleomorphic adenomas and carcinomas ex pleomorphic adenomas of the salivary glands. A total of 33 pleomorphic adenomas and 16 carcinomas ex pleomorphic adenomas of the salivary glands underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed quantitatively. For statistical analysis, Mann–Whitney test was performed and a significance level was set at
p
≤ 0.05. All analyzed tumors (
n
= 49) were positive for apurinic/apyrimidinic endonuclease 1. However, there was a higher median expression in carcinomas ex pleomorphic adenomas (
p
< 0.001). There was no difference between this protein immunoexpression and tumors of major or minor salivary gland. Overexpression was found mainly in cases of carcinomas ex pleomorphic adenomas with lymph node metastasis (
p
= 0.002) and invasive growth (
p
= 0.003), when compared to cases without metastasis and without capsular invasion (intracapsular pattern). Our findings revealed that apurinic/apyrimidinic endonuclease 1 is downregulated in pleomorphic adenomas and overexpressed in carcinomas ex pleomorphic adenomas, suggesting that this protein is possibly deregulated in pleomorphic adenoma malignant transformation. Furthermore, the increased expression of this protein is associated with a more aggressive behavior in carcinomas ex pleomorphic adenomas, which suggests that this protein may represent a prognostic biomarker in the studied salivary gland tumors.</description><subject>Adenoma, Pleomorphic - genetics</subject><subject>Adenoma, Pleomorphic - pathology</subject><subject>Adult</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - pathology</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Head and Neck</subject><subject>Head and Neck Surgery</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neurosurgery</subject><subject>Otorhinolaryngology</subject><subject>Predictive Value of Tests</subject><subject>Proteomics - methods</subject><subject>Salivary Gland Neoplasms - genetics</subject><subject>Salivary Gland Neoplasms - pathology</subject><subject>Salivary Glands - pathology</subject><issn>0937-4477</issn><issn>1434-4726</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv3CAQhVHVqtls8gN6iTimB3cHA4t9XEVpUilSe8gdYRhviWxwwI66l_z2stqkx3IZ0HvzmPkI-cLgGwNQmwwgQFbAVCW25dJ-ICsmuKiEqrcfyQpaXhSh1Bk5z_kJAKRo-WdyVjey5oKxFXndTUvywduNmQ7Jj94dHxSDi2GxA5qMlNHr3a9b9pX6TOMLJvwzJcwZHfWBjmbw-2DCTOdkQu5jGs3sY6Cxp7loLyYd6H4wwdFpwDjGNP0uHxiHIY7mgnzqzZDx8q2uyeP328eb--rh592Pm91DZQWDuTKyk5Zb1zRWOVaDa6VUrrPS9OV0SjHnaq7YVqqWi7pTsrEtK26BTVH5mlyfYqcUnxfMsx59tjiUsTAuWbMWoOFNU0OxspPVpphzwl5PBUtZQjPQR-r6RF0X6vpIXbel5-otfulGdP863jEXQ30y5CKFPSb9FJcUysb_Sf0LOYaPJQ</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Silva, Leorik Pereira</creator><creator>Santana, Thalita</creator><creator>Sedassari, Bruno Tavares</creator><creator>de Sousa, Suzana Machado</creator><creator>Sobral, Ana Paula Veras</creator><creator>Freitas, Roseana de Almeida</creator><creator>Barboza, Carlos Augusto Galvão</creator><creator>de Souza, Lélia Batista</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in malignant transformation of salivary gland pleomorphic adenoma</title><author>Silva, Leorik Pereira ; Santana, Thalita ; Sedassari, Bruno Tavares ; de Sousa, Suzana Machado ; Sobral, Ana Paula Veras ; Freitas, Roseana de Almeida ; Barboza, Carlos Augusto Galvão ; de Souza, Lélia Batista</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-a5b5c3cd88c7d120d9557dbc5affffb771dd23716579342b758c9188c4e8b773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenoma, Pleomorphic - genetics</topic><topic>Adenoma, Pleomorphic - pathology</topic><topic>Adult</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - pathology</topic><topic>Cell Transformation, Neoplastic - genetics</topic><topic>DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Head and Neck</topic><topic>Head and Neck Surgery</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Neurosurgery</topic><topic>Otorhinolaryngology</topic><topic>Predictive Value of Tests</topic><topic>Proteomics - methods</topic><topic>Salivary Gland Neoplasms - genetics</topic><topic>Salivary Gland Neoplasms - pathology</topic><topic>Salivary Glands - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Leorik Pereira</creatorcontrib><creatorcontrib>Santana, Thalita</creatorcontrib><creatorcontrib>Sedassari, Bruno Tavares</creatorcontrib><creatorcontrib>de Sousa, Suzana Machado</creatorcontrib><creatorcontrib>Sobral, Ana Paula Veras</creatorcontrib><creatorcontrib>Freitas, Roseana de Almeida</creatorcontrib><creatorcontrib>Barboza, Carlos Augusto Galvão</creatorcontrib><creatorcontrib>de Souza, Lélia Batista</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European archives of oto-rhino-laryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Leorik Pereira</au><au>Santana, Thalita</au><au>Sedassari, Bruno Tavares</au><au>de Sousa, Suzana Machado</au><au>Sobral, Ana Paula Veras</au><au>Freitas, Roseana de Almeida</au><au>Barboza, Carlos Augusto Galvão</au><au>de Souza, Lélia Batista</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in malignant transformation of salivary gland pleomorphic adenoma</atitle><jtitle>European archives of oto-rhino-laryngology</jtitle><stitle>Eur Arch Otorhinolaryngol</stitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>274</volume><issue>8</issue><spage>3203</spage><epage>3209</epage><pages>3203-3209</pages><issn>0937-4477</issn><eissn>1434-4726</eissn><abstract>DNA repair systems play a critical role in protecting the human genome against cumulative damage. The apurinic/apyrimidinic endonuclease 1 is a protein involved in DNA base excision repair and its expression still needs to be investigated in salivary gland tumors. The objective of this study is to analyze the immunoexpression of apurinic/apyrimidinic endonuclease 1 in pleomorphic adenomas and carcinomas ex pleomorphic adenomas of the salivary glands. A total of 33 pleomorphic adenomas and 16 carcinomas ex pleomorphic adenomas of the salivary glands underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed quantitatively. For statistical analysis, Mann–Whitney test was performed and a significance level was set at
p
≤ 0.05. All analyzed tumors (
n
= 49) were positive for apurinic/apyrimidinic endonuclease 1. However, there was a higher median expression in carcinomas ex pleomorphic adenomas (
p
< 0.001). There was no difference between this protein immunoexpression and tumors of major or minor salivary gland. Overexpression was found mainly in cases of carcinomas ex pleomorphic adenomas with lymph node metastasis (
p
= 0.002) and invasive growth (
p
= 0.003), when compared to cases without metastasis and without capsular invasion (intracapsular pattern). Our findings revealed that apurinic/apyrimidinic endonuclease 1 is downregulated in pleomorphic adenomas and overexpressed in carcinomas ex pleomorphic adenomas, suggesting that this protein is possibly deregulated in pleomorphic adenoma malignant transformation. Furthermore, the increased expression of this protein is associated with a more aggressive behavior in carcinomas ex pleomorphic adenomas, which suggests that this protein may represent a prognostic biomarker in the studied salivary gland tumors.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28523411</pmid><doi>10.1007/s00405-017-4605-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | European archives of oto-rhino-laryngology, 2017-08, Vol.274 (8), p.3203-3209 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adenoma, Pleomorphic - genetics Adenoma, Pleomorphic - pathology Adult Carcinoma - genetics Carcinoma - pathology Cell Transformation, Neoplastic - genetics DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics Female Gene Expression Profiling Head and Neck Head and Neck Surgery Humans Immunohistochemistry Male Medicine Medicine & Public Health Middle Aged Neurosurgery Otorhinolaryngology Predictive Value of Tests Proteomics - methods Salivary Gland Neoplasms - genetics Salivary Gland Neoplasms - pathology Salivary Glands - pathology |
| title | Apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in malignant transformation of salivary gland pleomorphic adenoma |
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