Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory
Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as st...
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| Veröffentlicht in: | Bone & joint research 2017-05, Vol.6 (5), p.315-322 |
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| creator | Martinez-Perez, M Perez-Jorge, C Lozano, D Portal-Nuñez, S Perez-Tanoira, R Conde, A Arenas, M A Hernandez-Lopez, J M de Damborenea, J J Gomez-Barrena, E Esbrit, P Esteban, J |
| description | Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the
studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied.
We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis.
Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria.
The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.
M. Martinez-Perez, C. Perez-Jorge, D. Lozano, S. Portal-Nuñez, R. Perez-Tanoira, A. Conde, M. A. Arenas, J. M. Hernandez-Lopez, J. J. de Damborenea, E. Gomez-Barrena, P. Esbrit, J. Esteban. Evaluation of bacterial adherence of clinical isolates of
using a competitive model: An
approach to the "race for the surface" theory.
2017;6:315-322. DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2. |
| doi_str_mv | 10.1302/2046-3758.65.BJR-2016-0226.R2 |
| format | Article |
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studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied.
We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis.
Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria.
The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.
M. Martinez-Perez, C. Perez-Jorge, D. Lozano, S. Portal-Nuñez, R. Perez-Tanoira, A. Conde, M. A. Arenas, J. M. Hernandez-Lopez, J. J. de Damborenea, E. Gomez-Barrena, P. Esbrit, J. Esteban. Evaluation of bacterial adherence of clinical isolates of
using a competitive model: An
approach to the "race for the surface" theory.
2017;6:315-322. DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2.</description><identifier>ISSN: 2046-3758</identifier><identifier>EISSN: 2046-3758</identifier><identifier>DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2</identifier><identifier>PMID: 28522445</identifier><language>eng</language><publisher>England: British Editorial Society of Bone & Joint Surgery</publisher><subject>Bacteria ; Clinical isolates ; Image processing ; Staphylococcus ; Strains (organisms) ; Surgery</subject><ispartof>Bone & joint research, 2017-05, Vol.6 (5), p.315-322</ispartof><rights>2017 Martinez-Perez et al.</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c268t-4408d23ac1ba8045ba90747791d746a0e6728c9142da74ab37f7a446d281c30b3</citedby><cites>FETCH-LOGICAL-c268t-4408d23ac1ba8045ba90747791d746a0e6728c9142da74ab37f7a446d281c30b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28522445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Perez, M</creatorcontrib><creatorcontrib>Perez-Jorge, C</creatorcontrib><creatorcontrib>Lozano, D</creatorcontrib><creatorcontrib>Portal-Nuñez, S</creatorcontrib><creatorcontrib>Perez-Tanoira, R</creatorcontrib><creatorcontrib>Conde, A</creatorcontrib><creatorcontrib>Arenas, M A</creatorcontrib><creatorcontrib>Hernandez-Lopez, J M</creatorcontrib><creatorcontrib>de Damborenea, J J</creatorcontrib><creatorcontrib>Gomez-Barrena, E</creatorcontrib><creatorcontrib>Esbrit, P</creatorcontrib><creatorcontrib>Esteban, J</creatorcontrib><title>Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory</title><title>Bone & joint research</title><addtitle>Bone Joint Res</addtitle><description>Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the
studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied.
We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis.
Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria.
The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.
M. Martinez-Perez, C. Perez-Jorge, D. Lozano, S. Portal-Nuñez, R. Perez-Tanoira, A. Conde, M. A. Arenas, J. M. Hernandez-Lopez, J. J. de Damborenea, E. Gomez-Barrena, P. Esbrit, J. Esteban. Evaluation of bacterial adherence of clinical isolates of
using a competitive model: An
approach to the "race for the surface" theory.
2017;6:315-322. DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2.</description><subject>Bacteria</subject><subject>Clinical isolates</subject><subject>Image processing</subject><subject>Staphylococcus</subject><subject>Strains (organisms)</subject><subject>Surgery</subject><issn>2046-3758</issn><issn>2046-3758</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpdkd1qFTEUhYMothz7ChIqgjcz5m-SHMGLWlp_KAhHvQ57MhlPysxkTDIHzuP4pmbaWsRcJFkra2dv-BB6TUlNOWFvGRGy4qrRtWzqD192FSNUVoQxWe_YE3T6-P70n_sJOkvplpQltZBEP0cnTDeMCdGcot9XBxgWyD5MOPS4BZtd9DBg6PYuusm61baDn7wtrk9hgOzSan7LMO-PQ7DB2iXhNNd4SX76iQHbMM4u--wPDo-hc8M7fDFhP-GDzzFgmOcYwO5xDjjvHT6PUPr0Id6ptMS-6PNVhHh8gZ71MCR39nBu0I_rq--Xn6qbrx8_X17cVJZJnSshiO4YB0tb0EQ0LWyJEkptaaeEBOKkYtpuqWAdKAEtV70CIWTHNLWctHyD3tz_W2b7tbiUzeiTdcMAkwtLMnRLiOaKlW2DXv0XvQ1LnMp0hnFKhKCM8ZJ6f5-yMaQUXW_m6EeIR0OJWXGaFZFZERnZmILTrDjNitPsWKl_-dBlaUfXPVb_hcf_AB0CnJQ</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Martinez-Perez, M</creator><creator>Perez-Jorge, C</creator><creator>Lozano, D</creator><creator>Portal-Nuñez, S</creator><creator>Perez-Tanoira, R</creator><creator>Conde, A</creator><creator>Arenas, M A</creator><creator>Hernandez-Lopez, J M</creator><creator>de Damborenea, J J</creator><creator>Gomez-Barrena, E</creator><creator>Esbrit, P</creator><creator>Esteban, J</creator><general>British Editorial Society of Bone & Joint Surgery</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory</title><author>Martinez-Perez, M ; Perez-Jorge, C ; Lozano, D ; Portal-Nuñez, S ; Perez-Tanoira, R ; Conde, A ; Arenas, M A ; Hernandez-Lopez, J M ; de Damborenea, J J ; Gomez-Barrena, E ; Esbrit, P ; Esteban, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-4408d23ac1ba8045ba90747791d746a0e6728c9142da74ab37f7a446d281c30b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Bacteria</topic><topic>Clinical isolates</topic><topic>Image processing</topic><topic>Staphylococcus</topic><topic>Strains (organisms)</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Perez, M</creatorcontrib><creatorcontrib>Perez-Jorge, C</creatorcontrib><creatorcontrib>Lozano, D</creatorcontrib><creatorcontrib>Portal-Nuñez, S</creatorcontrib><creatorcontrib>Perez-Tanoira, R</creatorcontrib><creatorcontrib>Conde, A</creatorcontrib><creatorcontrib>Arenas, M A</creatorcontrib><creatorcontrib>Hernandez-Lopez, J M</creatorcontrib><creatorcontrib>de Damborenea, J J</creatorcontrib><creatorcontrib>Gomez-Barrena, E</creatorcontrib><creatorcontrib>Esbrit, P</creatorcontrib><creatorcontrib>Esteban, J</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Bone & joint research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez-Perez, M</au><au>Perez-Jorge, C</au><au>Lozano, D</au><au>Portal-Nuñez, S</au><au>Perez-Tanoira, R</au><au>Conde, A</au><au>Arenas, M A</au><au>Hernandez-Lopez, J M</au><au>de Damborenea, J J</au><au>Gomez-Barrena, E</au><au>Esbrit, P</au><au>Esteban, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory</atitle><jtitle>Bone & joint research</jtitle><addtitle>Bone Joint Res</addtitle><date>2017-05</date><risdate>2017</risdate><volume>6</volume><issue>5</issue><spage>315</spage><epage>322</epage><pages>315-322</pages><issn>2046-3758</issn><eissn>2046-3758</eissn><abstract>Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the
studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied.
We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis.
Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria.
The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.
M. Martinez-Perez, C. Perez-Jorge, D. Lozano, S. Portal-Nuñez, R. Perez-Tanoira, A. Conde, M. A. Arenas, J. M. Hernandez-Lopez, J. J. de Damborenea, E. Gomez-Barrena, P. Esbrit, J. Esteban. Evaluation of bacterial adherence of clinical isolates of
using a competitive model: An
approach to the "race for the surface" theory.
2017;6:315-322. DOI: 10.1302/2046-3758.65.BJR-2016-0226.R2.</abstract><cop>England</cop><pub>British Editorial Society of Bone & Joint Surgery</pub><pmid>28522445</pmid><doi>10.1302/2046-3758.65.BJR-2016-0226.R2</doi><tpages>8</tpages></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Bacteria Clinical isolates Image processing Staphylococcus Strains (organisms) Surgery |
| title | Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory |
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