In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization influenced by application of various oils
•Intercellular lipids order profile measured by confocal Raman microscopy in vivo.•Topically applied oils influence the intercellular lipids order in stratum corneum.•Oils do not permeate through the stratum corneum in vivo.•Plant-derived oils influence deeper-located layers more than mineral-derive...
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| Veröffentlicht in: | Journal of dermatological science 2017-08, Vol.87 (2), p.183-191 |
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| creator | Choe, ChunSik Schleusener, Johannes Lademann, Jürgen Darvin, Maxim E. |
| description | •Intercellular lipids order profile measured by confocal Raman microscopy in vivo.•Topically applied oils influence the intercellular lipids order in stratum corneum.•Oils do not permeate through the stratum corneum in vivo.•Plant-derived oils influence deeper-located layers more than mineral-derived oils.
The intercellular lipids (ICL) of stratum corneum (SC) play an important role in maintaining the skin barrier function. The lateral and lamellar packing order of ICL in SC is not homogenous, but rather depth-dependent.
This study aimed to analyze the influence of the topically applied mineral-derived (paraffin and petrolatum) and plant-derived (almond oil and jojoba oil) oils on the depth-dependent ICL profile ordering of the SC in vivo.
Confocal Raman microscopy (CRM), a unique tool to analyze the depth profile of the ICL structure non-invasively, is employed to investigate the interaction between oils and human SC in vivo.
The results show that the response of SC to oils’ permeation varies in the depths. All oils remain in the upper layers of the SC (0–20% of SC thickness) and show predominated differences of ICL ordering from intact skin. In these depths, skin treated with plant-derived oils shows more disordered lateral and lamellar packing order of ICL than intact skin (p0.1), except plant-derived oils at the depth 30% of SC thickness. In the deeper layers of the SC (60–100% of SC thickness), no difference between ICL lateral packing order of the oil-treated and intact skin can be observed, except that at the depths of 70–90% of the SC thickness, where slight changes with more disorder states are measured for plant-derived oil treated skin (p |
| doi_str_mv | 10.1016/j.jdermsci.2017.04.016 |
| format | Article |
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The intercellular lipids (ICL) of stratum corneum (SC) play an important role in maintaining the skin barrier function. The lateral and lamellar packing order of ICL in SC is not homogenous, but rather depth-dependent.
This study aimed to analyze the influence of the topically applied mineral-derived (paraffin and petrolatum) and plant-derived (almond oil and jojoba oil) oils on the depth-dependent ICL profile ordering of the SC in vivo.
Confocal Raman microscopy (CRM), a unique tool to analyze the depth profile of the ICL structure non-invasively, is employed to investigate the interaction between oils and human SC in vivo.
The results show that the response of SC to oils’ permeation varies in the depths. All oils remain in the upper layers of the SC (0–20% of SC thickness) and show predominated differences of ICL ordering from intact skin. In these depths, skin treated with plant-derived oils shows more disordered lateral and lamellar packing order of ICL than intact skin (p<0.05). In the intermediate layers of SC (30–50% of SC thickness), the oils do not influence the lateral packing order of SC ICL (p>0.1), except plant-derived oils at the depth 30% of SC thickness. In the deeper layers of the SC (60–100% of SC thickness), no difference between ICL lateral packing order of the oil-treated and intact skin can be observed, except that at the depths of 70–90% of the SC thickness, where slight changes with more disorder states are measured for plant-derived oil treated skin (p<0.1), which could be explained by the penetration of free fatty acid fractions in the deep-located SC areas.
Both oil types remain in the superficial layers of the SC (0–20% of the SC thickness). Skin treated with mineral- and plant-derived oils shows significantly higher disordered lateral and lamellar packing order of ICL in these layers of the SC compared to intact skin. Plant-derived oils significantly changed the ICL ordering in the depths of 30% and 70–90% of the SC thickness, which is likely due to the penetration of free fatty acids in the deeper layers of the SC.</description><identifier>ISSN: 0923-1811</identifier><identifier>EISSN: 1873-569X</identifier><identifier>DOI: 10.1016/j.jdermsci.2017.04.016</identifier><identifier>PMID: 28522139</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; almond oil ; Epidermis - metabolism ; Female ; Healthy Volunteers ; Humans ; intercellular lipids ; jojoba oil ; keratin ; Lipid Metabolism ; Male ; Microscopy, Confocal - methods ; Middle Aged ; Mineral Oil - pharmacology ; non-invasive measurements ; ordered/disordered lipids ; paraffin ; Paraffin - pharmacology ; petrolatum ; Plant Oils - pharmacology ; Skin Absorption ; skin barrier function ; Spectrum Analysis, Raman - methods ; Waxes - pharmacology ; Young Adult</subject><ispartof>Journal of dermatological science, 2017-08, Vol.87 (2), p.183-191</ispartof><rights>2017 Japanese Society for Investigative Dermatology</rights><rights>Copyright © 2017 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-792c85e1c5f89b75958377faa1d9eb69e6ddcf99de170e718fb1f2358eaf631d3</citedby><cites>FETCH-LOGICAL-c506t-792c85e1c5f89b75958377faa1d9eb69e6ddcf99de170e718fb1f2358eaf631d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jdermsci.2017.04.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28522139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choe, ChunSik</creatorcontrib><creatorcontrib>Schleusener, Johannes</creatorcontrib><creatorcontrib>Lademann, Jürgen</creatorcontrib><creatorcontrib>Darvin, Maxim E.</creatorcontrib><title>In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization influenced by application of various oils</title><title>Journal of dermatological science</title><addtitle>J Dermatol Sci</addtitle><description>•Intercellular lipids order profile measured by confocal Raman microscopy in vivo.•Topically applied oils influence the intercellular lipids order in stratum corneum.•Oils do not permeate through the stratum corneum in vivo.•Plant-derived oils influence deeper-located layers more than mineral-derived oils.
The intercellular lipids (ICL) of stratum corneum (SC) play an important role in maintaining the skin barrier function. The lateral and lamellar packing order of ICL in SC is not homogenous, but rather depth-dependent.
This study aimed to analyze the influence of the topically applied mineral-derived (paraffin and petrolatum) and plant-derived (almond oil and jojoba oil) oils on the depth-dependent ICL profile ordering of the SC in vivo.
Confocal Raman microscopy (CRM), a unique tool to analyze the depth profile of the ICL structure non-invasively, is employed to investigate the interaction between oils and human SC in vivo.
The results show that the response of SC to oils’ permeation varies in the depths. All oils remain in the upper layers of the SC (0–20% of SC thickness) and show predominated differences of ICL ordering from intact skin. In these depths, skin treated with plant-derived oils shows more disordered lateral and lamellar packing order of ICL than intact skin (p<0.05). In the intermediate layers of SC (30–50% of SC thickness), the oils do not influence the lateral packing order of SC ICL (p>0.1), except plant-derived oils at the depth 30% of SC thickness. In the deeper layers of the SC (60–100% of SC thickness), no difference between ICL lateral packing order of the oil-treated and intact skin can be observed, except that at the depths of 70–90% of the SC thickness, where slight changes with more disorder states are measured for plant-derived oil treated skin (p<0.1), which could be explained by the penetration of free fatty acid fractions in the deep-located SC areas.
Both oil types remain in the superficial layers of the SC (0–20% of the SC thickness). Skin treated with mineral- and plant-derived oils shows significantly higher disordered lateral and lamellar packing order of ICL in these layers of the SC compared to intact skin. Plant-derived oils significantly changed the ICL ordering in the depths of 30% and 70–90% of the SC thickness, which is likely due to the penetration of free fatty acids in the deeper layers of the SC.</description><subject>Adult</subject><subject>almond oil</subject><subject>Epidermis - metabolism</subject><subject>Female</subject><subject>Healthy Volunteers</subject><subject>Humans</subject><subject>intercellular lipids</subject><subject>jojoba oil</subject><subject>keratin</subject><subject>Lipid Metabolism</subject><subject>Male</subject><subject>Microscopy, Confocal - methods</subject><subject>Middle Aged</subject><subject>Mineral Oil - pharmacology</subject><subject>non-invasive measurements</subject><subject>ordered/disordered lipids</subject><subject>paraffin</subject><subject>Paraffin - pharmacology</subject><subject>petrolatum</subject><subject>Plant Oils - pharmacology</subject><subject>Skin Absorption</subject><subject>skin barrier function</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Waxes - pharmacology</subject><subject>Young Adult</subject><issn>0923-1811</issn><issn>1873-569X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1r3DAQFaWh2Sb9C0HHXuxI9sq2bi2haQOBQmkgNyFLo2YWWXIleyH5If291XaTXHsaeLyPmXmEXHBWc8a7y129s5CmbLBuGO9rtq0L_IZs-NC3lejk_VuyYbJpKz5wfkre57xjjIlmK9-R02YQTcNbuSF_bgLd4z5SE4OLRnv6Q0860AlNitnEGQ21sJQoDHrBGGh0BZiXBzqn6NBDPiDLA9C8JL2sU3FKAcr0OKOlMf3SAZ-OWgzOrxAMWDo-Uj3PHs2r614njGuxQ5_PyYnTPsOH53lG7q6__Lz6Vt1-_3pz9fm2MoJ1S9XLxgwCuBFukGMvpBjavndacyth7CR01honpQXeM-j54EbumlYMoF3XctuekY9H33LM7xXyoibMBrzXAcouikvGiiWX20LtjtTDY3ICp-aEk06PijN16ETt1Esn6tCJYltV4CK8eM5Yxwnsq-ylhEL4dCRAuXSPkFSx-PclTGAWZSP-L-MvsOalqA</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Choe, ChunSik</creator><creator>Schleusener, Johannes</creator><creator>Lademann, Jürgen</creator><creator>Darvin, Maxim E.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization influenced by application of various oils</title><author>Choe, ChunSik ; Schleusener, Johannes ; Lademann, Jürgen ; Darvin, Maxim E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-792c85e1c5f89b75958377faa1d9eb69e6ddcf99de170e718fb1f2358eaf631d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>almond oil</topic><topic>Epidermis - metabolism</topic><topic>Female</topic><topic>Healthy Volunteers</topic><topic>Humans</topic><topic>intercellular lipids</topic><topic>jojoba oil</topic><topic>keratin</topic><topic>Lipid Metabolism</topic><topic>Male</topic><topic>Microscopy, Confocal - methods</topic><topic>Middle Aged</topic><topic>Mineral Oil - pharmacology</topic><topic>non-invasive measurements</topic><topic>ordered/disordered lipids</topic><topic>paraffin</topic><topic>Paraffin - pharmacology</topic><topic>petrolatum</topic><topic>Plant Oils - pharmacology</topic><topic>Skin Absorption</topic><topic>skin barrier function</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Waxes - pharmacology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choe, ChunSik</creatorcontrib><creatorcontrib>Schleusener, Johannes</creatorcontrib><creatorcontrib>Lademann, Jürgen</creatorcontrib><creatorcontrib>Darvin, Maxim E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dermatological science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choe, ChunSik</au><au>Schleusener, Johannes</au><au>Lademann, Jürgen</au><au>Darvin, Maxim E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization influenced by application of various oils</atitle><jtitle>Journal of dermatological science</jtitle><addtitle>J Dermatol Sci</addtitle><date>2017-08</date><risdate>2017</risdate><volume>87</volume><issue>2</issue><spage>183</spage><epage>191</epage><pages>183-191</pages><issn>0923-1811</issn><eissn>1873-569X</eissn><abstract>•Intercellular lipids order profile measured by confocal Raman microscopy in vivo.•Topically applied oils influence the intercellular lipids order in stratum corneum.•Oils do not permeate through the stratum corneum in vivo.•Plant-derived oils influence deeper-located layers more than mineral-derived oils.
The intercellular lipids (ICL) of stratum corneum (SC) play an important role in maintaining the skin barrier function. The lateral and lamellar packing order of ICL in SC is not homogenous, but rather depth-dependent.
This study aimed to analyze the influence of the topically applied mineral-derived (paraffin and petrolatum) and plant-derived (almond oil and jojoba oil) oils on the depth-dependent ICL profile ordering of the SC in vivo.
Confocal Raman microscopy (CRM), a unique tool to analyze the depth profile of the ICL structure non-invasively, is employed to investigate the interaction between oils and human SC in vivo.
The results show that the response of SC to oils’ permeation varies in the depths. All oils remain in the upper layers of the SC (0–20% of SC thickness) and show predominated differences of ICL ordering from intact skin. In these depths, skin treated with plant-derived oils shows more disordered lateral and lamellar packing order of ICL than intact skin (p<0.05). In the intermediate layers of SC (30–50% of SC thickness), the oils do not influence the lateral packing order of SC ICL (p>0.1), except plant-derived oils at the depth 30% of SC thickness. In the deeper layers of the SC (60–100% of SC thickness), no difference between ICL lateral packing order of the oil-treated and intact skin can be observed, except that at the depths of 70–90% of the SC thickness, where slight changes with more disorder states are measured for plant-derived oil treated skin (p<0.1), which could be explained by the penetration of free fatty acid fractions in the deep-located SC areas.
Both oil types remain in the superficial layers of the SC (0–20% of the SC thickness). Skin treated with mineral- and plant-derived oils shows significantly higher disordered lateral and lamellar packing order of ICL in these layers of the SC compared to intact skin. Plant-derived oils significantly changed the ICL ordering in the depths of 30% and 70–90% of the SC thickness, which is likely due to the penetration of free fatty acids in the deeper layers of the SC.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28522139</pmid><doi>10.1016/j.jdermsci.2017.04.016</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult almond oil Epidermis - metabolism Female Healthy Volunteers Humans intercellular lipids jojoba oil keratin Lipid Metabolism Male Microscopy, Confocal - methods Middle Aged Mineral Oil - pharmacology non-invasive measurements ordered/disordered lipids paraffin Paraffin - pharmacology petrolatum Plant Oils - pharmacology Skin Absorption skin barrier function Spectrum Analysis, Raman - methods Waxes - pharmacology Young Adult |
| title | In vivo confocal Raman microscopic determination of depth profiles of the stratum corneum lipid organization influenced by application of various oils |
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