Transcriptomic profiling reveals disordered regulation of surfactant homeostasis in neonatal cloned bovines with collapsed lungs and respiratory distress

Respiratory distress is a major cause of mortality in cloned neonatal animals, but its pathogenesis remains poorly understood. Here, we used necropsy and histology procedures to evaluate the lungs of cloned neonatal bovines dying of respiratory distress, finding incomplete lung dilation, alveolar co...

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Veröffentlicht in:	Molecular reproduction and development 2017-08, Vol.84 (8), p.668-674
Hauptverfasser:	Liu, Yan, Rao, Yifan, Jiang, Xiaojing, Zhang, Fanyi, Huang, Linhua, Du, Weihua, Hao, Haisheng, Zhao, Xueming, Wang, Dong, Jiang, Qiuling, Zhu, Huabin, Sun, Xiuzhu
Format:	Artikel
Sprache:	eng
Schlagworte:	Alveoli Animals Animals, Newborn Biosynthesis Cattle Cloning Cloning, Organism Degradation Female Gene expression Gene Expression Profiling - methods Histology Homeostasis Homeostasis - genetics Immunohistochemistry Lung - chemistry Lung - metabolism lung collapse Lungs MAP kinase Mortality neonatal cloned bovines Neonates Notch protein Nuclear transfer Oligonucleotide Array Sequence Analysis Packing Protein B Protein C Proteins Proteins - analysis Proteins - genetics Proteins - metabolism Pulmonary Atelectasis - metabolism pulmonary surfactant Pulmonary Surfactants - analysis Pulmonary Surfactants - metabolism Real-Time Polymerase Chain Reaction respiratory distress Respiratory Distress Syndrome, Newborn - metabolism Secretion Somatic cell nuclear transfer surfactant homeostasis Surfactant protein C Surfactants Transcription Transcriptome - genetics
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Zusammenfassung:	Respiratory distress is a major cause of mortality in cloned neonatal animals, but its pathogenesis remains poorly understood. Here, we used necropsy and histology procedures to evaluate the lungs of cloned neonatal bovines dying of respiratory distress, finding incomplete lung dilation, alveolar collapse, and thickened alveolar walls. Comparison of the transcriptomes between collapsed lungs of cloned bovines and their normal counterparts revealed 1373 differentially expressed genes in collapsed lungs (p 1.5 or
ISSN:	1040-452X 1098-2795
DOI:	10.1002/mrd.22836