Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells
Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify...
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| Veröffentlicht in: | Tissue engineering. Part C, Methods Methods, 2017-05, Vol.23 (5), p.298-310 |
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| creator | Ribitsch, Iris Chang-Rodriguez, Souyet Egerbacher, Monika Gabner, Simone Gueltekin, Sinan Huber, Johann Schuster, Therese Jenner, Florien |
| description | Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs. Therefore, in the present study, ovine placenta cotyledons, which have similar function and structure to human chorionic villi, were tested for their potential use as a noninvasive source of ovine MSCs. Through mincing of the placental cotyledons, collagenase digestion, and Ficoll density gradient centrifugation, combined with plastic adherence selection, MSCs were successfully isolated. Their morphological, immunophenotypical, and cellular growth characteristics, as well as their proliferation, differentiation, and migration potential, were evaluated and compared to the currently best-researched MSC source, bone marrow-derived stem cells. Ovine cotyledons were shown to be a reliable, abundant source for the noninvasive, pain- and risk-free harvest of MSCs. The collection procedure does not interfere with partum or the initial bonding phase between ewes and lambs and is therefore exempt from ethical debate. Ovine placenta cotyledon-derived MSCs exhibit multipotential characteristics and can be cryopreserved for later use. |
| doi_str_mv | 10.1089/ten.tec.2017.0067 |
| format | Article |
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However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs. Therefore, in the present study, ovine placenta cotyledons, which have similar function and structure to human chorionic villi, were tested for their potential use as a noninvasive source of ovine MSCs. Through mincing of the placental cotyledons, collagenase digestion, and Ficoll density gradient centrifugation, combined with plastic adherence selection, MSCs were successfully isolated. Their morphological, immunophenotypical, and cellular growth characteristics, as well as their proliferation, differentiation, and migration potential, were evaluated and compared to the currently best-researched MSC source, bone marrow-derived stem cells. Ovine cotyledons were shown to be a reliable, abundant source for the noninvasive, pain- and risk-free harvest of MSCs. The collection procedure does not interfere with partum or the initial bonding phase between ewes and lambs and is therefore exempt from ethical debate. Ovine placenta cotyledon-derived MSCs exhibit multipotential characteristics and can be cryopreserved for later use.</description><identifier>ISSN: 1937-3384</identifier><identifier>EISSN: 1937-3392</identifier><identifier>DOI: 10.1089/ten.tec.2017.0067</identifier><identifier>PMID: 28504918</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Adipogenesis - physiology ; Adipose tissue ; Amnion ; Amniotic fluid ; Anatomy ; Animal models ; Animals ; Antibiotics ; Bacteria ; Biochemistry ; Blood circulation ; Blood vessels ; Bone growth ; Bone marrow ; Bone matrix ; Cartilage ; Cell culture ; Cell Differentiation ; Cell Movement ; Cell Proliferation ; Cell Separation - methods ; Chondrogenesis - physiology ; Clinical trials ; Contamination ; Disinfectants ; Embryology ; Female ; Fetuses ; Histology ; Horses ; Immunogenicity ; Launching ; Life span ; Membranes ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Osteogenesis - physiology ; Pain ; Parturition ; Placenta ; Placenta - cytology ; Placenta - physiology ; Pregnancy ; Sheep ; Stem cells ; Tissue Engineering - methods ; Tissues ; Umbilical cord</subject><ispartof>Tissue engineering. 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Part C, Methods</title><addtitle>Tissue Eng Part C Methods</addtitle><description>Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs. Therefore, in the present study, ovine placenta cotyledons, which have similar function and structure to human chorionic villi, were tested for their potential use as a noninvasive source of ovine MSCs. Through mincing of the placental cotyledons, collagenase digestion, and Ficoll density gradient centrifugation, combined with plastic adherence selection, MSCs were successfully isolated. Their morphological, immunophenotypical, and cellular growth characteristics, as well as their proliferation, differentiation, and migration potential, were evaluated and compared to the currently best-researched MSC source, bone marrow-derived stem cells. Ovine cotyledons were shown to be a reliable, abundant source for the noninvasive, pain- and risk-free harvest of MSCs. The collection procedure does not interfere with partum or the initial bonding phase between ewes and lambs and is therefore exempt from ethical debate. Ovine placenta cotyledon-derived MSCs exhibit multipotential characteristics and can be cryopreserved for later use.</description><subject>Adipogenesis - physiology</subject><subject>Adipose tissue</subject><subject>Amnion</subject><subject>Amniotic fluid</subject><subject>Anatomy</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Blood circulation</subject><subject>Blood vessels</subject><subject>Bone growth</subject><subject>Bone marrow</subject><subject>Bone matrix</subject><subject>Cartilage</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cell Separation - methods</subject><subject>Chondrogenesis - physiology</subject><subject>Clinical trials</subject><subject>Contamination</subject><subject>Disinfectants</subject><subject>Embryology</subject><subject>Female</subject><subject>Fetuses</subject><subject>Histology</subject><subject>Horses</subject><subject>Immunogenicity</subject><subject>Launching</subject><subject>Life span</subject><subject>Membranes</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Osteogenesis - physiology</subject><subject>Pain</subject><subject>Parturition</subject><subject>Placenta</subject><subject>Placenta - cytology</subject><subject>Placenta - physiology</subject><subject>Pregnancy</subject><subject>Sheep</subject><subject>Stem cells</subject><subject>Tissue Engineering - methods</subject><subject>Tissues</subject><subject>Umbilical cord</subject><issn>1937-3384</issn><issn>1937-3392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkE1LAzEQhoMoVqs_wIsEvHhpTTabTeJNil9QrVA9L9npBLfsZutmW-i_N0u1B08ewoThmZeZh5ALzsacaXPToR93COOEcTVmLFMH5IQboUZCmORw_9fpgJyGsIxEZMwxGSRastRwfUJm80_EFX2rLKDvLJ003bbCRePDLb2jr40v_caGcoN03qxbQNo4OtuUHukLBvTwua1tRecd1nSCVRXOyJGzVcDznzokHw_375On0XT2-Dy5m45AKNWN0KUFOraATIhMOs0SkIIDKmlASBACJUimQEOxsElqnEgzxQpQTnLHVCaG5HqXu2qbrzWGLq_LAHED67FZh5xrY1KeGsEievUHXcZTfNyup7RKuZY6UnxHQduE0KLLV21Z23abc5b3tvNoOz7Ie9t5bzvOXP4kr4saF_uJX70RUDugb1vvqxILbLt_RH8DprWOKA</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Ribitsch, Iris</creator><creator>Chang-Rodriguez, Souyet</creator><creator>Egerbacher, Monika</creator><creator>Gabner, Simone</creator><creator>Gueltekin, Sinan</creator><creator>Huber, Johann</creator><creator>Schuster, Therese</creator><creator>Jenner, Florien</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells</title><author>Ribitsch, Iris ; 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Part C, Methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribitsch, Iris</au><au>Chang-Rodriguez, Souyet</au><au>Egerbacher, Monika</au><au>Gabner, Simone</au><au>Gueltekin, Sinan</au><au>Huber, Johann</au><au>Schuster, Therese</au><au>Jenner, Florien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells</atitle><jtitle>Tissue engineering. Part C, Methods</jtitle><addtitle>Tissue Eng Part C Methods</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>23</volume><issue>5</issue><spage>298</spage><epage>310</epage><pages>298-310</pages><issn>1937-3384</issn><eissn>1937-3392</eissn><abstract>Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs. Therefore, in the present study, ovine placenta cotyledons, which have similar function and structure to human chorionic villi, were tested for their potential use as a noninvasive source of ovine MSCs. Through mincing of the placental cotyledons, collagenase digestion, and Ficoll density gradient centrifugation, combined with plastic adherence selection, MSCs were successfully isolated. Their morphological, immunophenotypical, and cellular growth characteristics, as well as their proliferation, differentiation, and migration potential, were evaluated and compared to the currently best-researched MSC source, bone marrow-derived stem cells. Ovine cotyledons were shown to be a reliable, abundant source for the noninvasive, pain- and risk-free harvest of MSCs. The collection procedure does not interfere with partum or the initial bonding phase between ewes and lambs and is therefore exempt from ethical debate. Ovine placenta cotyledon-derived MSCs exhibit multipotential characteristics and can be cryopreserved for later use.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>28504918</pmid><doi>10.1089/ten.tec.2017.0067</doi><tpages>13</tpages></addata></record> |
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| ispartof | Tissue engineering. Part C, Methods, 2017-05, Vol.23 (5), p.298-310 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipogenesis - physiology Adipose tissue Amnion Amniotic fluid Anatomy Animal models Animals Antibiotics Bacteria Biochemistry Blood circulation Blood vessels Bone growth Bone marrow Bone matrix Cartilage Cell culture Cell Differentiation Cell Movement Cell Proliferation Cell Separation - methods Chondrogenesis - physiology Clinical trials Contamination Disinfectants Embryology Female Fetuses Histology Horses Immunogenicity Launching Life span Membranes Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Osteogenesis - physiology Pain Parturition Placenta Placenta - cytology Placenta - physiology Pregnancy Sheep Stem cells Tissue Engineering - methods Tissues Umbilical cord |
| title | Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells |
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