Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging
A novel Förster resonance energy transfer (FRET) fluorescence "off-on" system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthes...
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| Veröffentlicht in: | Chemical science (Cambridge) 2017-03, Vol.8 (3), p.2199-2203 |
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| container_title | Chemical science (Cambridge) |
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| creator | Li, Hua Zhang, Deliang Gao, Mengna Huang, Lumei Tang, Longguang Li, Zijing Chen, Xiaoyuan Zhang, Xianzhong |
| description | A novel Förster resonance energy transfer (FRET) fluorescence "off-on" system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and [4'-(
,
'-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The
imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of
semiquantitative fluorescence imaging of NO in mammals. |
| doi_str_mv | 10.1039/c6sc04071c |
| format | Article |
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,
'-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The
imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of
semiquantitative fluorescence imaging of NO in mammals.</description><identifier>ISSN: 2041-6520</identifier><identifier>EISSN: 2041-6539</identifier><identifier>DOI: 10.1039/c6sc04071c</identifier><identifier>PMID: 28507674</identifier><language>eng</language><publisher>England</publisher><subject>Carbon-carbon composites ; Chemical bonds ; Cleavage ; Fluorescence ; Fretting ; Imaging ; Mice ; Nitric oxide</subject><ispartof>Chemical science (Cambridge), 2017-03, Vol.8 (3), p.2199-2203</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-1dc7646eba7d0c45de1d684791b07cb324764768dab41829db1bdf869e9e1a373</citedby><cites>FETCH-LOGICAL-c397t-1dc7646eba7d0c45de1d684791b07cb324764768dab41829db1bdf869e9e1a373</cites><orcidid>0000-0001-8591-8301 ; 0000-0002-9622-0870</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28507674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Zhang, Deliang</creatorcontrib><creatorcontrib>Gao, Mengna</creatorcontrib><creatorcontrib>Huang, Lumei</creatorcontrib><creatorcontrib>Tang, Longguang</creatorcontrib><creatorcontrib>Li, Zijing</creatorcontrib><creatorcontrib>Chen, Xiaoyuan</creatorcontrib><creatorcontrib>Zhang, Xianzhong</creatorcontrib><title>Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging</title><title>Chemical science (Cambridge)</title><addtitle>Chem Sci</addtitle><description>A novel Förster resonance energy transfer (FRET) fluorescence "off-on" system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and [4'-(
,
'-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The
imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of
semiquantitative fluorescence imaging of NO in mammals.</description><subject>Carbon-carbon composites</subject><subject>Chemical bonds</subject><subject>Cleavage</subject><subject>Fluorescence</subject><subject>Fretting</subject><subject>Imaging</subject><subject>Mice</subject><subject>Nitric oxide</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNUU1LAzEQDaJYqb34AyRHEVaTTTbZHGVprVAQtJ6XfO0aSTd10y323xtt7dm5zMB785h5D4ArjO4wIuJes6gRRRzrE3CRI4ozVhBxepxzNAKTGD9QKkJwkfNzMMrLAnHG6QVo56599zsY11a7xmlYZRVUoTNQeyu3srXQdWbQ1sDZy3QJGz-E3kZtO21hE_qEwq3bBqhc8KF1WnrYuU2flMKXM2l7JVvXtZfgrJE-2smhj8HbbLqs5tni-fGpelhkmgi-ybDRnFFmleQGaVoYiw0rKRdYIa4VyWmCOSuNVBSXuTAKK9OUTFhhsSScjMHNXnfdh8_Bxk29cula72VnwxBrXApBUSkw_Qe1pBjnNNk2Brd7qu5DjL1t6nWfHut3NUb1Twx1xV6r3xiqRL4-6A5qZc2R-mc6-QZ6_IGD</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Li, Hua</creator><creator>Zhang, Deliang</creator><creator>Gao, Mengna</creator><creator>Huang, Lumei</creator><creator>Tang, Longguang</creator><creator>Li, Zijing</creator><creator>Chen, Xiaoyuan</creator><creator>Zhang, Xianzhong</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8591-8301</orcidid><orcidid>https://orcid.org/0000-0002-9622-0870</orcidid></search><sort><creationdate>20170301</creationdate><title>Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging</title><author>Li, Hua ; Zhang, Deliang ; Gao, Mengna ; Huang, Lumei ; Tang, Longguang ; Li, Zijing ; Chen, Xiaoyuan ; Zhang, Xianzhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-1dc7646eba7d0c45de1d684791b07cb324764768dab41829db1bdf869e9e1a373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Carbon-carbon composites</topic><topic>Chemical bonds</topic><topic>Cleavage</topic><topic>Fluorescence</topic><topic>Fretting</topic><topic>Imaging</topic><topic>Mice</topic><topic>Nitric oxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Zhang, Deliang</creatorcontrib><creatorcontrib>Gao, Mengna</creatorcontrib><creatorcontrib>Huang, Lumei</creatorcontrib><creatorcontrib>Tang, Longguang</creatorcontrib><creatorcontrib>Li, Zijing</creatorcontrib><creatorcontrib>Chen, Xiaoyuan</creatorcontrib><creatorcontrib>Zhang, Xianzhong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Hua</au><au>Zhang, Deliang</au><au>Gao, Mengna</au><au>Huang, Lumei</au><au>Tang, Longguang</au><au>Li, Zijing</au><au>Chen, Xiaoyuan</au><au>Zhang, Xianzhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging</atitle><jtitle>Chemical science (Cambridge)</jtitle><addtitle>Chem Sci</addtitle><date>2017-03-01</date><risdate>2017</risdate><volume>8</volume><issue>3</issue><spage>2199</spage><epage>2203</epage><pages>2199-2203</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>A novel Förster resonance energy transfer (FRET) fluorescence "off-on" system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and [4'-(
,
'-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The
imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of
semiquantitative fluorescence imaging of NO in mammals.</abstract><cop>England</cop><pmid>28507674</pmid><doi>10.1039/c6sc04071c</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-8591-8301</orcidid><orcidid>https://orcid.org/0000-0002-9622-0870</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Carbon-carbon composites Chemical bonds Cleavage Fluorescence Fretting Imaging Mice Nitric oxide |
| title | Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging |
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