Salivary Immunoglobulin Gene Expression in Patients with Caries
BACKGROUNDImmunoglobulins mediate the host's humoral immune response are expressed in saliva. AIMTo quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODSThis was a comparative cross-sectional st...
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| Veröffentlicht in: | Open access Macedonian journal of medical sciences 2017, Vol.5 (2), p.236-243 |
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| container_title | Open access Macedonian journal of medical sciences |
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| creator | Santín, Gema Regina Guadarrama Salgado, Angel Visoso Bastida, Norma Margarita Montiel Gómez, Isaías de la Rosa Benítez, Jonnathan Guadalupe Santillán Zerón, Hugo Mendieta |
| description | BACKGROUNDImmunoglobulins mediate the host's humoral immune response are expressed in saliva. AIMTo quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODSThis was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTSOne hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSIONAlthough we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found. |
| doi_str_mv | 10.3889/oamjms.2017.028 |
| format | Report |
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AIMTo quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODSThis was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTSOne hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSIONAlthough we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found.</description><identifier>ISSN: 1857-9655</identifier><identifier>EISSN: 1857-9655</identifier><identifier>DOI: 10.3889/oamjms.2017.028</identifier><language>eng</language><ispartof>Open access Macedonian journal of medical sciences, 2017, Vol.5 (2), p.236-243</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>777,781,4477,27907</link.rule.ids></links><search><creatorcontrib>Santín, Gema Regina Guadarrama</creatorcontrib><creatorcontrib>Salgado, Angel Visoso</creatorcontrib><creatorcontrib>Bastida, Norma Margarita Montiel</creatorcontrib><creatorcontrib>Gómez, Isaías de la Rosa</creatorcontrib><creatorcontrib>Benítez, Jonnathan Guadalupe Santillán</creatorcontrib><creatorcontrib>Zerón, Hugo Mendieta</creatorcontrib><title>Salivary Immunoglobulin Gene Expression in Patients with Caries</title><title>Open access Macedonian journal of medical sciences</title><description>BACKGROUNDImmunoglobulins mediate the host's humoral immune response are expressed in saliva. AIMTo quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODSThis was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTSOne hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSIONAlthough we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found.</description><issn>1857-9655</issn><issn>1857-9655</issn><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>2017</creationdate><recordtype>report</recordtype><recordid>eNqVijsPgjAYABujiUSdXTu6gC0ItJMD8bWZ6G6q-dSSPpAPfPx7HRxcne5yOULGnEWJEHLqlS0tRjHjecRi0SEBF2keyixNuz_eJyPEkjHGU5nxOA7IfKeMvqv6RTfWts5fjD-2Rju6Agd08axqQNTe0U_aqkaDa5A-dHOlhao14JD0zsogjL4ckMlysS_WYVX7WwvYHKzGExijHPgWD1xIOWNCJFnyx_oGUC5FGA</recordid><startdate>20170415</startdate><enddate>20170415</enddate><creator>Santín, Gema Regina Guadarrama</creator><creator>Salgado, Angel Visoso</creator><creator>Bastida, Norma Margarita Montiel</creator><creator>Gómez, Isaías de la Rosa</creator><creator>Benítez, Jonnathan Guadalupe Santillán</creator><creator>Zerón, Hugo Mendieta</creator><scope>7X8</scope></search><sort><creationdate>20170415</creationdate><title>Salivary Immunoglobulin Gene Expression in Patients with Caries</title><author>Santín, Gema Regina Guadarrama ; Salgado, Angel Visoso ; Bastida, Norma Margarita Montiel ; Gómez, Isaías de la Rosa ; Benítez, Jonnathan Guadalupe Santillán ; Zerón, Hugo Mendieta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_18994088363</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Santín, Gema Regina Guadarrama</creatorcontrib><creatorcontrib>Salgado, Angel Visoso</creatorcontrib><creatorcontrib>Bastida, Norma Margarita Montiel</creatorcontrib><creatorcontrib>Gómez, Isaías de la Rosa</creatorcontrib><creatorcontrib>Benítez, Jonnathan Guadalupe Santillán</creatorcontrib><creatorcontrib>Zerón, Hugo Mendieta</creatorcontrib><collection>MEDLINE - Academic</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santín, Gema Regina Guadarrama</au><au>Salgado, Angel Visoso</au><au>Bastida, Norma Margarita Montiel</au><au>Gómez, Isaías de la Rosa</au><au>Benítez, Jonnathan Guadalupe Santillán</au><au>Zerón, Hugo Mendieta</au><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><atitle>Salivary Immunoglobulin Gene Expression in Patients with Caries</atitle><jtitle>Open access Macedonian journal of medical sciences</jtitle><date>2017-04-15</date><risdate>2017</risdate><volume>5</volume><issue>2</issue><spage>236</spage><epage>243</epage><pages>236-243</pages><issn>1857-9655</issn><eissn>1857-9655</eissn><abstract>BACKGROUNDImmunoglobulins mediate the host's humoral immune response are expressed in saliva. AIMTo quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODSThis was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTSOne hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSIONAlthough we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found.</abstract><doi>10.3889/oamjms.2017.028</doi></addata></record> |
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| title | Salivary Immunoglobulin Gene Expression in Patients with Caries |
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