Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)
Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel da...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2017-11, Vol.183 (3), p.980-992 |
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| creator | Choudhary, S. B. Chowdhury, I. Singh, R. K. Pandey, S. P. Sharma, H. K. Anil Kumar, A. Karmakar, P. G. Kumari, N. Souframanien, J. Jambhulkar, S. J. |
| description | Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel dark jute (
Corchorus olitorius
L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of
CCoAMT1
gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme. |
| doi_str_mv | 10.1007/s12010-017-2477-5 |
| format | Article |
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Corchorus olitorius
L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of
CCoAMT1
gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-017-2477-5</identifier><identifier>PMID: 28497373</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemical analysis ; Biochemistry ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Corchorus - cytology ; Corchorus - genetics ; Corchorus - growth & development ; Corchorus - metabolism ; Corchorus aestuans ; Corchorus olitorius ; Developmental biology ; Fibers ; Gamma rays ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Genes, Plant - genetics ; Industrial applications ; Jute ; Lignin ; Lignin - biosynthesis ; Lignin - metabolism ; Metabolites ; Morphology ; Mutants ; Mutation ; Phloem ; Phloem - cytology ; Phloem - genetics ; Phloem - growth & development ; Phloem - metabolism ; Plant cells ; Plant Stems - growth & development ; Polymerase chain reaction ; Transcription ; Transcription, Genetic</subject><ispartof>Applied biochemistry and biotechnology, 2017-11, Vol.183 (3), p.980-992</ispartof><rights>Springer Science+Business Media New York 2017</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-c8c834cc9e441467f32cc427cdca7651baf93b38afbe140a1c9ff0fbd716d0dd3</citedby><cites>FETCH-LOGICAL-c409t-c8c834cc9e441467f32cc427cdca7651baf93b38afbe140a1c9ff0fbd716d0dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-017-2477-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-017-2477-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28497373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choudhary, S. B.</creatorcontrib><creatorcontrib>Chowdhury, I.</creatorcontrib><creatorcontrib>Singh, R. K.</creatorcontrib><creatorcontrib>Pandey, S. P.</creatorcontrib><creatorcontrib>Sharma, H. K.</creatorcontrib><creatorcontrib>Anil Kumar, A.</creatorcontrib><creatorcontrib>Karmakar, P. G.</creatorcontrib><creatorcontrib>Kumari, N.</creatorcontrib><creatorcontrib>Souframanien, J.</creatorcontrib><creatorcontrib>Jambhulkar, S. J.</creatorcontrib><title>Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel dark jute (
Corchorus olitorius
L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of
CCoAMT1
gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme.</description><subject>Biochemical analysis</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Corchorus - cytology</subject><subject>Corchorus - genetics</subject><subject>Corchorus - growth & development</subject><subject>Corchorus - metabolism</subject><subject>Corchorus aestuans</subject><subject>Corchorus olitorius</subject><subject>Developmental biology</subject><subject>Fibers</subject><subject>Gamma rays</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Genes, Plant - genetics</subject><subject>Industrial applications</subject><subject>Jute</subject><subject>Lignin</subject><subject>Lignin - biosynthesis</subject><subject>Lignin - metabolism</subject><subject>Metabolites</subject><subject>Morphology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Phloem</subject><subject>Phloem - cytology</subject><subject>Phloem - genetics</subject><subject>Phloem - growth & development</subject><subject>Phloem - metabolism</subject><subject>Plant cells</subject><subject>Plant Stems - growth & development</subject><subject>Polymerase chain reaction</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFu1DAQhi0EokvhAbggS1xaiRSP48TxES2UgrKCA5wtx7E3Lk68tR0hHoJ3xqstCCFx8sjzzT8jfQg9B3IFhPDXCSgBUhHgFWWcV80DtIGmERWhAh6iDaG8rijtxBl6ktItIUC7hj9GZ7Rjgte83qCfuxAPU_Bh77Tyr_CNSzkMLujJzMcfrJYR74I3evUq4u2kotLZRJdUdmHBweI-fMe92y9uwZ8nH8yMr90QDb7w_mAv8W7NaslH8K2K3_DHNZfWNkQ9hbgmHLzLIbpS9VeXT9Ejq3wyz-7fc_T1-t2X7U3Vf3r_YfumrzQjIle6013NtBaGMWAttzXVmlGuR61428CgrKiHulN2MMCIAi2sJXYYObQjGcf6HF2ccg8x3K0mZTm7pI33ajFhTRI6IQBayrqCvvwHvQ1rXMp1EkTDOWnrFgoFJ0rHkFI0Vh6im1X8IYHIoyt5ciWLK3l0JZsy8-I-eR1mM_6Z-C2nAPQEpNJa9ib-tfq_qb8AlFef8g</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Choudhary, S. B.</creator><creator>Chowdhury, I.</creator><creator>Singh, R. K.</creator><creator>Pandey, S. P.</creator><creator>Sharma, H. K.</creator><creator>Anil Kumar, A.</creator><creator>Karmakar, P. G.</creator><creator>Kumari, N.</creator><creator>Souframanien, J.</creator><creator>Jambhulkar, S. J.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20171101</creationdate><title>Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)</title><author>Choudhary, S. B. ; Chowdhury, I. ; Singh, R. K. ; Pandey, S. P. ; Sharma, H. K. ; Anil Kumar, A. ; Karmakar, P. G. ; Kumari, N. ; Souframanien, J. ; Jambhulkar, S. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-c8c834cc9e441467f32cc427cdca7651baf93b38afbe140a1c9ff0fbd716d0dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biochemical analysis</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Corchorus - cytology</topic><topic>Corchorus - genetics</topic><topic>Corchorus - growth & development</topic><topic>Corchorus - metabolism</topic><topic>Corchorus aestuans</topic><topic>Corchorus olitorius</topic><topic>Developmental biology</topic><topic>Fibers</topic><topic>Gamma rays</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Genes, Plant - genetics</topic><topic>Industrial applications</topic><topic>Jute</topic><topic>Lignin</topic><topic>Lignin - biosynthesis</topic><topic>Lignin - metabolism</topic><topic>Metabolites</topic><topic>Morphology</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Phloem</topic><topic>Phloem - cytology</topic><topic>Phloem - genetics</topic><topic>Phloem - growth & development</topic><topic>Phloem - metabolism</topic><topic>Plant cells</topic><topic>Plant Stems - growth & development</topic><topic>Polymerase chain reaction</topic><topic>Transcription</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choudhary, S. B.</creatorcontrib><creatorcontrib>Chowdhury, I.</creatorcontrib><creatorcontrib>Singh, R. K.</creatorcontrib><creatorcontrib>Pandey, S. P.</creatorcontrib><creatorcontrib>Sharma, H. K.</creatorcontrib><creatorcontrib>Anil Kumar, A.</creatorcontrib><creatorcontrib>Karmakar, P. G.</creatorcontrib><creatorcontrib>Kumari, N.</creatorcontrib><creatorcontrib>Souframanien, J.</creatorcontrib><creatorcontrib>Jambhulkar, S. 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B.</au><au>Chowdhury, I.</au><au>Singh, R. K.</au><au>Pandey, S. P.</au><au>Sharma, H. K.</au><au>Anil Kumar, A.</au><au>Karmakar, P. G.</au><au>Kumari, N.</au><au>Souframanien, J.</au><au>Jambhulkar, S. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>183</volume><issue>3</issue><spage>980</spage><epage>992</epage><pages>980-992</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel dark jute (
Corchorus olitorius
L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of
CCoAMT1
gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28497373</pmid><doi>10.1007/s12010-017-2477-5</doi><tpages>13</tpages></addata></record> |
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| subjects | Biochemical analysis Biochemistry Biotechnology Chemistry Chemistry and Materials Science Corchorus - cytology Corchorus - genetics Corchorus - growth & development Corchorus - metabolism Corchorus aestuans Corchorus olitorius Developmental biology Fibers Gamma rays Gene expression Gene Expression Regulation, Plant Gene regulation Genes, Plant - genetics Industrial applications Jute Lignin Lignin - biosynthesis Lignin - metabolism Metabolites Morphology Mutants Mutation Phloem Phloem - cytology Phloem - genetics Phloem - growth & development Phloem - metabolism Plant cells Plant Stems - growth & development Polymerase chain reaction Transcription Transcription, Genetic |
| title | Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.) |
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