Transforming growth factor beta-1 (TGF-β1) stimulates collagen synthesis in cultured rainbow trout cardiac fibroblasts
Cold acclimation of rainbow trout, , causes collagen to increase within the extracellular matrix (ECM) of the myocardium, while warm acclimation has the opposite effect. The mechanism responsible for this remodelling response is not known. In mammals, transforming growth factor beta-1 (TGF-β1) stimu...
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| Veröffentlicht in: | Journal of experimental biology 2017-07, Vol.220 (Pt 14), p.2645-2653 |
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| creator | Johnston, Elizabeth F Gillis, Todd E |
| description | Cold acclimation of rainbow trout,
, causes collagen to increase within the extracellular matrix (ECM) of the myocardium, while warm acclimation has the opposite effect. The mechanism responsible for this remodelling response is not known. In mammals, transforming growth factor beta-1 (TGF-β1) stimulates collagen deposition within the myocardial ECM. Therefore, we hypothesized that TGF-β1 regulates trout myocardial ECM turnover and predicted that TGF-β1 would induce collagen deposition in cultured rainbow trout cardiac fibroblasts. We found that treatment of trout cardiac fibroblasts with 15 ng ml
human recombinant TGF-β1 caused an increase in total collagen at 48 and 72 h and an increase in collagen type I protein after 7 days. We also found that TGF-β1 treatment caused an increase in the transcript abundance of tissue inhibitor of metalloproteinase 2 (
) and matrix metalloproteinase 9 (
) at 24 h. Cells treated with TGF-β1 also had lower levels of the gene transcript for
after 48 h and higher levels of the gene transcript for collagen type I α1 (
) after 72 h. These changes in gene expression suggest that the increase in collagen deposition is due to a decrease in the activity of matrix metalloproteinases and an increase in collagen synthesis. Together, these results indicate that TGF-β1 is a regulator of ECM composition in cultured trout cardiac fibroblasts and suggest that this cytokine may play a role in regulating collagen content in the trout heart during thermal acclimation. |
| doi_str_mv | 10.1242/jeb.160093 |
| format | Article |
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, causes collagen to increase within the extracellular matrix (ECM) of the myocardium, while warm acclimation has the opposite effect. The mechanism responsible for this remodelling response is not known. In mammals, transforming growth factor beta-1 (TGF-β1) stimulates collagen deposition within the myocardial ECM. Therefore, we hypothesized that TGF-β1 regulates trout myocardial ECM turnover and predicted that TGF-β1 would induce collagen deposition in cultured rainbow trout cardiac fibroblasts. We found that treatment of trout cardiac fibroblasts with 15 ng ml
human recombinant TGF-β1 caused an increase in total collagen at 48 and 72 h and an increase in collagen type I protein after 7 days. We also found that TGF-β1 treatment caused an increase in the transcript abundance of tissue inhibitor of metalloproteinase 2 (
) and matrix metalloproteinase 9 (
) at 24 h. Cells treated with TGF-β1 also had lower levels of the gene transcript for
after 48 h and higher levels of the gene transcript for collagen type I α1 (
) after 72 h. These changes in gene expression suggest that the increase in collagen deposition is due to a decrease in the activity of matrix metalloproteinases and an increase in collagen synthesis. Together, these results indicate that TGF-β1 is a regulator of ECM composition in cultured trout cardiac fibroblasts and suggest that this cytokine may play a role in regulating collagen content in the trout heart during thermal acclimation.</description><identifier>ISSN: 0022-0949</identifier><identifier>EISSN: 1477-9145</identifier><identifier>DOI: 10.1242/jeb.160093</identifier><identifier>PMID: 28495868</identifier><language>eng</language><publisher>England: The Company of Biologists Ltd</publisher><subject>Acclimation ; Acclimatization ; Animals ; Cells, Cultured ; Cold acclimation ; Collagen ; Collagen (type I) ; Collagen - biosynthesis ; Cytokines ; Deposition ; Extracellular matrix ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Gelatinase A ; Gelatinase B ; Gene Expression ; Growth factors ; Heart ; Heart diseases ; Matrix metalloproteinase ; Matrix Metalloproteinase 2 - analysis ; Matrix metalloproteinases ; Metalloproteinase ; Myocardium ; Myocardium - metabolism ; Oncorhynchus mykiss ; Salmon ; Synthesis ; Tissue inhibitor of metalloproteinase 2 ; Tissue Inhibitor of Metalloproteinase-2 - analysis ; Transcription ; Transforming Growth Factor beta1 - pharmacology ; Transforming growth factor-b1 ; Trout</subject><ispartof>Journal of experimental biology, 2017-07, Vol.220 (Pt 14), p.2645-2653</ispartof><rights>2017. Published by The Company of Biologists Ltd.</rights><rights>Copyright The Company of Biologists Ltd Jul 15, 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-dc9a8e2b2a70e29010490adaae6baf69fdd7db8995b03bbcfab6e902fec0a0753</citedby><cites>FETCH-LOGICAL-c417t-dc9a8e2b2a70e29010490adaae6baf69fdd7db8995b03bbcfab6e902fec0a0753</cites><orcidid>0000-0002-8585-0658</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28495868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnston, Elizabeth F</creatorcontrib><creatorcontrib>Gillis, Todd E</creatorcontrib><title>Transforming growth factor beta-1 (TGF-β1) stimulates collagen synthesis in cultured rainbow trout cardiac fibroblasts</title><title>Journal of experimental biology</title><addtitle>J Exp Biol</addtitle><description>Cold acclimation of rainbow trout,
, causes collagen to increase within the extracellular matrix (ECM) of the myocardium, while warm acclimation has the opposite effect. The mechanism responsible for this remodelling response is not known. In mammals, transforming growth factor beta-1 (TGF-β1) stimulates collagen deposition within the myocardial ECM. Therefore, we hypothesized that TGF-β1 regulates trout myocardial ECM turnover and predicted that TGF-β1 would induce collagen deposition in cultured rainbow trout cardiac fibroblasts. We found that treatment of trout cardiac fibroblasts with 15 ng ml
human recombinant TGF-β1 caused an increase in total collagen at 48 and 72 h and an increase in collagen type I protein after 7 days. We also found that TGF-β1 treatment caused an increase in the transcript abundance of tissue inhibitor of metalloproteinase 2 (
) and matrix metalloproteinase 9 (
) at 24 h. Cells treated with TGF-β1 also had lower levels of the gene transcript for
after 48 h and higher levels of the gene transcript for collagen type I α1 (
) after 72 h. These changes in gene expression suggest that the increase in collagen deposition is due to a decrease in the activity of matrix metalloproteinases and an increase in collagen synthesis. Together, these results indicate that TGF-β1 is a regulator of ECM composition in cultured trout cardiac fibroblasts and suggest that this cytokine may play a role in regulating collagen content in the trout heart during thermal acclimation.</description><subject>Acclimation</subject><subject>Acclimatization</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cold acclimation</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Collagen - biosynthesis</subject><subject>Cytokines</subject><subject>Deposition</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Gelatinase A</subject><subject>Gelatinase B</subject><subject>Gene Expression</subject><subject>Growth factors</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 2 - analysis</subject><subject>Matrix metalloproteinases</subject><subject>Metalloproteinase</subject><subject>Myocardium</subject><subject>Myocardium - metabolism</subject><subject>Oncorhynchus mykiss</subject><subject>Salmon</subject><subject>Synthesis</subject><subject>Tissue inhibitor of metalloproteinase 2</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - analysis</subject><subject>Transcription</subject><subject>Transforming Growth Factor beta1 - pharmacology</subject><subject>Transforming growth factor-b1</subject><subject>Trout</subject><issn>0022-0949</issn><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1uFDEQhC1ERJbAhQdAlrgEpAltz599jCISkCLlsjmP2p72xquZcbA9WuW1eBCeKUYbOFCXvnxdKlUx9kHAhZCN_LoncyE6AF2_YhvR9H2lRdO-ZhsAKSvQjT5lb1PaQ1HXNm_YqVSNblWnNuywjbgkF-Lslx3fxXDID9yhzSFyQxkrwc-3N9fV71_iM0_Zz-uEmRK3YZpwRwtPT0t-oOQT9wu365TXSCOP6BcTDjzHsGZuMY4eLXfexGAmTDm9YycOp0TvX-4Zu7_-tr36Xt3e3fy4urytbCP6XI1WoyJpJPZAUoOARgOOiNQZdJ1249iPRmndGqiNsQ5NRxqkIwsIfVufsfOj72MMP1dKeZh9slTCLxTWNIjyK4p6VdBP_6H7sMalpBtkKU4pJWoo1JcjZWNIKZIbHqOfMT4NAoY_cwxljuE4R4E_vliuZqbxH_q3__oZLRmIKg</recordid><startdate>20170715</startdate><enddate>20170715</enddate><creator>Johnston, Elizabeth F</creator><creator>Gillis, Todd E</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8585-0658</orcidid></search><sort><creationdate>20170715</creationdate><title>Transforming growth factor beta-1 (TGF-β1) stimulates collagen synthesis in cultured rainbow trout cardiac fibroblasts</title><author>Johnston, Elizabeth F ; Gillis, Todd E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-dc9a8e2b2a70e29010490adaae6baf69fdd7db8995b03bbcfab6e902fec0a0753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acclimation</topic><topic>Acclimatization</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cold acclimation</topic><topic>Collagen</topic><topic>Collagen (type I)</topic><topic>Collagen - biosynthesis</topic><topic>Cytokines</topic><topic>Deposition</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Gelatinase A</topic><topic>Gelatinase B</topic><topic>Gene Expression</topic><topic>Growth factors</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 2 - analysis</topic><topic>Matrix metalloproteinases</topic><topic>Metalloproteinase</topic><topic>Myocardium</topic><topic>Myocardium - metabolism</topic><topic>Oncorhynchus mykiss</topic><topic>Salmon</topic><topic>Synthesis</topic><topic>Tissue inhibitor of metalloproteinase 2</topic><topic>Tissue Inhibitor of Metalloproteinase-2 - analysis</topic><topic>Transcription</topic><topic>Transforming Growth Factor beta1 - pharmacology</topic><topic>Transforming growth factor-b1</topic><topic>Trout</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnston, Elizabeth F</creatorcontrib><creatorcontrib>Gillis, Todd E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnston, Elizabeth F</au><au>Gillis, Todd E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transforming growth factor beta-1 (TGF-β1) stimulates collagen synthesis in cultured rainbow trout cardiac fibroblasts</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>2017-07-15</date><risdate>2017</risdate><volume>220</volume><issue>Pt 14</issue><spage>2645</spage><epage>2653</epage><pages>2645-2653</pages><issn>0022-0949</issn><eissn>1477-9145</eissn><abstract>Cold acclimation of rainbow trout,
, causes collagen to increase within the extracellular matrix (ECM) of the myocardium, while warm acclimation has the opposite effect. The mechanism responsible for this remodelling response is not known. In mammals, transforming growth factor beta-1 (TGF-β1) stimulates collagen deposition within the myocardial ECM. Therefore, we hypothesized that TGF-β1 regulates trout myocardial ECM turnover and predicted that TGF-β1 would induce collagen deposition in cultured rainbow trout cardiac fibroblasts. We found that treatment of trout cardiac fibroblasts with 15 ng ml
human recombinant TGF-β1 caused an increase in total collagen at 48 and 72 h and an increase in collagen type I protein after 7 days. We also found that TGF-β1 treatment caused an increase in the transcript abundance of tissue inhibitor of metalloproteinase 2 (
) and matrix metalloproteinase 9 (
) at 24 h. Cells treated with TGF-β1 also had lower levels of the gene transcript for
after 48 h and higher levels of the gene transcript for collagen type I α1 (
) after 72 h. These changes in gene expression suggest that the increase in collagen deposition is due to a decrease in the activity of matrix metalloproteinases and an increase in collagen synthesis. Together, these results indicate that TGF-β1 is a regulator of ECM composition in cultured trout cardiac fibroblasts and suggest that this cytokine may play a role in regulating collagen content in the trout heart during thermal acclimation.</abstract><cop>England</cop><pub>The Company of Biologists Ltd</pub><pmid>28495868</pmid><doi>10.1242/jeb.160093</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8585-0658</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acclimation Acclimatization Animals Cells, Cultured Cold acclimation Collagen Collagen (type I) Collagen - biosynthesis Cytokines Deposition Extracellular matrix Fibroblasts Fibroblasts - drug effects Fibroblasts - metabolism Gelatinase A Gelatinase B Gene Expression Growth factors Heart Heart diseases Matrix metalloproteinase Matrix Metalloproteinase 2 - analysis Matrix metalloproteinases Metalloproteinase Myocardium Myocardium - metabolism Oncorhynchus mykiss Salmon Synthesis Tissue inhibitor of metalloproteinase 2 Tissue Inhibitor of Metalloproteinase-2 - analysis Transcription Transforming Growth Factor beta1 - pharmacology Transforming growth factor-b1 Trout |
| title | Transforming growth factor beta-1 (TGF-β1) stimulates collagen synthesis in cultured rainbow trout cardiac fibroblasts |
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