Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies

The ability to simultaneously visualize the presence, abundance, location and functional state of many targets in cells and tissues has been described as a true next-generation approach in immunohistochemistry (IHC). A typical requirement for multiplex IHC (mIHC) is the use of different animal speci...

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Veröffentlicht in:	Laboratory investigation 2017-07, Vol.97 (7), p.873-885
Hauptverfasser:	Zhang, Wenjun, Hubbard, Antony, Jones, Tobin, Racolta, Adriana, Bhaumik, Srabani, Cummins, Nick, Zhang, Liping, Garsha, Karl, Ventura, Frank, Lefever, Mark R, Lu, Zhenqiang, Hurley, John K, Day, William A, Pestic-Dragovich, Lidija, Morrison, Larry E, Tang, Lei
Format:	Artikel
Sprache:	eng
Schlagworte:	13/51 13/56 631/1647/664/1257 692/53/2421 Abundance Amides - chemistry Amides - metabolism Animal species Antibodies Antibodies - chemistry Antibodies - metabolism Antigens Assaying Automation Biomarkers Bonding strength Breast - chemistry CD20 antigen CD3 antigen CD8 antigen Chemical compounds Covalence Cross-reactivity Deactivation Elution Epitopes Female Filters Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fluorophores Foxp3 protein Humans Image Processing, Computer-Assisted - methods Immunohistochemistry Immunohistochemistry - methods Laboratory Medicine Medicine Medicine & Public Health Multiplexing Neoplasms - chemistry Palatine Tonsil - chemistry Pathology Reproducibility Reproducibility of Results Species Staining technical-report Tissues
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