Effects of Gadd45[alpha]-knockdown on human extravillous trophoblast cell migration and invasion functions under hypoxia/re-oxygenation

Objective [middot] To investigate the effects of growth arrest and DNA damage 45 alpha(Gadd45[alpha]) on the migration and invasion function of human extravillous trophoblast cells under hypoxia/re-oxygenation(H/R). Methods [middot] Human extravillous trophoblast cells were infected by sh RNA lentivirus targeting Gadd45[alpha] gene, to knock down Gadd45[alpha] gene expression. Then the oxidative stress model of preeclampsia was used in vitro to observe the changes of cell biological functions. The experiments were divided into 4 groups, nontreated group, hypoxia/re-oxygenation group, sh RNA Gadd45[alpha]+H/R group and sh RNA negative control+H/R group. Human villous explant experiments were used to determine the effects of silencing Gadd45[alpha] on human extravillous trophoblast cell under oxidative stress. Protein expression of Gadd45[alpha] was identified by Western blotting. Changes of cell migration and invasion were detected by transwell migration and Matrigel invasion assay. Gelatin zymography was used