Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells
Background and Objectives The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8...
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| Veröffentlicht in: | Journal of periodontal research 2017-06, Vol.52 (3), p.582-593 |
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| creator | Aung, W. P. P. Chotjumlong, P. Pata, S. Montreekachon, P. Supanchart, C. Khongkhunthian, S. Sastraruji, T. Krisanaprakornkit, S. |
| description | Background and Objectives
The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chronic periodontitis tissues compared with that in normal tissues. ADAM8 expression and its inductive mechanism were examined in human gingival epithelial cells (HGECs) and human gingival fibroblasts.
Material and Methods
Total RNA and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. ADAM8 mRNA and protein expression was analyzed by real‐time polymerase chain reaction, immunoblotting and immunohistochemistry. ADAM8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen‐activated protein kinase pathways was assayed by real‐time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence.
Results
ADAM8 mRNA and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues (p < 0.01). Significantly increased ADAM8 expression was detected in the gingival epithelium of chronic periodontitis tissues (p < 0.001). ADAM8 mRNA expression in HGECs, but not in human gingival fibroblasts, was significantly induced by stimulation with Fusobacterium nucleatum (p < 0.05), partially via the p44/42 mitogen‐activated protein kinase pathway. ADAM8 expression in the cell lysates and on the surface of HGECs was induced by stimulation with F. nucleatum.
Conclusion
ADAM8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of ADAM8 expression in F. nucleatum‐stimulated HGECs, suggesting a possible role of ADAM8 in innate immunity of periodontal tissue. |
| doi_str_mv | 10.1111/jre.12426 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1897374588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1920515096</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3866-a8ddacdcf33630059adba8e4053bc2d1c884cee2ff02ff28741fc8375ed230c73</originalsourceid><addsrcrecordid>eNqNkV1rFTEQhoNY7LF64R-QgDf1Ytt8bD72stSqLRVB9DrkJLOnOeQka7Jr7b837aleCIIDw8yQZ14mvAi9ouSEtjjdFjihrGfyCVpRSUhHlBRP0YoQxjre6_4QPa91S9os1fAMHTKlxcAkWaH5MvnFhXUEDD-nArWGnHAe8Rl-F2pIM2xKSNgmjz_BbGPMU8kzhGQrYI3bk7spOQWHJygh-5zmMIf6sLAJaRN-2IhhCvMNxNBaBzHWF-hgtLHCy8d6hL69v_h6_rG7_vzh8vzsunNcS9lZ7b113o2cS06IGKxfWw09EXztmKdO694BsHEkLZlWPR2d5kqAZ5w4xY_Q8V633fx9gTqbXaj3F9gEeamG6kFx1Qut_wPtqRo416Khb_5Ct3kpqX3E0IERQQUZZKPe7ilXcq0FRjOVsLPlzlBi7l0zzTXz4FpjXz8qLusd-D_kb5sacLoHbkOEu38rmasvF3vJX-Nhohg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920515096</pqid></control><display><type>article</type><title>Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Aung, W. P. P. ; Chotjumlong, P. ; Pata, S. ; Montreekachon, P. ; Supanchart, C. ; Khongkhunthian, S. ; Sastraruji, T. ; Krisanaprakornkit, S.</creator><creatorcontrib>Aung, W. P. P. ; Chotjumlong, P. ; Pata, S. ; Montreekachon, P. ; Supanchart, C. ; Khongkhunthian, S. ; Sastraruji, T. ; Krisanaprakornkit, S.</creatorcontrib><description>Background and Objectives
The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chronic periodontitis tissues compared with that in normal tissues. ADAM8 expression and its inductive mechanism were examined in human gingival epithelial cells (HGECs) and human gingival fibroblasts.
Material and Methods
Total RNA and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. ADAM8 mRNA and protein expression was analyzed by real‐time polymerase chain reaction, immunoblotting and immunohistochemistry. ADAM8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen‐activated protein kinase pathways was assayed by real‐time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence.
Results
ADAM8 mRNA and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues (p < 0.01). Significantly increased ADAM8 expression was detected in the gingival epithelium of chronic periodontitis tissues (p < 0.001). ADAM8 mRNA expression in HGECs, but not in human gingival fibroblasts, was significantly induced by stimulation with Fusobacterium nucleatum (p < 0.05), partially via the p44/42 mitogen‐activated protein kinase pathway. ADAM8 expression in the cell lysates and on the surface of HGECs was induced by stimulation with F. nucleatum.
Conclusion
ADAM8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of ADAM8 expression in F. nucleatum‐stimulated HGECs, suggesting a possible role of ADAM8 in innate immunity of periodontal tissue.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/jre.12426</identifier><identifier>PMID: 27859260</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>A Disintegrin and Metalloproteinase 8 ; ADAM protein ; ADAM Proteins - metabolism ; ADAM8 protein ; Adult ; Blotting, Western ; Case-Control Studies ; chronic periodontitis ; Chronic Periodontitis - metabolism ; Dentistry ; Epithelial cells ; Epithelial Cells - metabolism ; Epithelium ; Fibroblasts ; Flow Cytometry ; Fluorescent Antibody Technique ; Fusobacterium nucleatum ; Gene expression ; Gingiva ; Gingiva - cytology ; Gingiva - metabolism ; Gum disease ; Humans ; Immunoblotting ; Immunofluorescence ; Immunohistochemistry ; Inflammatory diseases ; Innate immunity ; Kinases ; Lysates ; MAP kinase ; Matrix Metalloproteinase 8 - metabolism ; Membrane Proteins - metabolism ; Middle Aged ; Neutrophil collagenase ; Periodontitis ; Polymerase chain reaction ; Protein expression ; Protein kinase ; Proteins ; Real-Time Polymerase Chain Reaction ; Young Adult</subject><ispartof>Journal of periodontal research, 2017-06, Vol.52 (3), p.582-593</ispartof><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3866-a8ddacdcf33630059adba8e4053bc2d1c884cee2ff02ff28741fc8375ed230c73</citedby><cites>FETCH-LOGICAL-c3866-a8ddacdcf33630059adba8e4053bc2d1c884cee2ff02ff28741fc8375ed230c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjre.12426$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjre.12426$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27859260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aung, W. P. P.</creatorcontrib><creatorcontrib>Chotjumlong, P.</creatorcontrib><creatorcontrib>Pata, S.</creatorcontrib><creatorcontrib>Montreekachon, P.</creatorcontrib><creatorcontrib>Supanchart, C.</creatorcontrib><creatorcontrib>Khongkhunthian, S.</creatorcontrib><creatorcontrib>Sastraruji, T.</creatorcontrib><creatorcontrib>Krisanaprakornkit, S.</creatorcontrib><title>Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objectives
The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chronic periodontitis tissues compared with that in normal tissues. ADAM8 expression and its inductive mechanism were examined in human gingival epithelial cells (HGECs) and human gingival fibroblasts.
Material and Methods
Total RNA and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. ADAM8 mRNA and protein expression was analyzed by real‐time polymerase chain reaction, immunoblotting and immunohistochemistry. ADAM8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen‐activated protein kinase pathways was assayed by real‐time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence.
Results
ADAM8 mRNA and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues (p < 0.01). Significantly increased ADAM8 expression was detected in the gingival epithelium of chronic periodontitis tissues (p < 0.001). ADAM8 mRNA expression in HGECs, but not in human gingival fibroblasts, was significantly induced by stimulation with Fusobacterium nucleatum (p < 0.05), partially via the p44/42 mitogen‐activated protein kinase pathway. ADAM8 expression in the cell lysates and on the surface of HGECs was induced by stimulation with F. nucleatum.
Conclusion
ADAM8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of ADAM8 expression in F. nucleatum‐stimulated HGECs, suggesting a possible role of ADAM8 in innate immunity of periodontal tissue.</description><subject>A Disintegrin and Metalloproteinase 8</subject><subject>ADAM protein</subject><subject>ADAM Proteins - metabolism</subject><subject>ADAM8 protein</subject><subject>Adult</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>chronic periodontitis</subject><subject>Chronic Periodontitis - metabolism</subject><subject>Dentistry</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium</subject><subject>Fibroblasts</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fusobacterium nucleatum</subject><subject>Gene expression</subject><subject>Gingiva</subject><subject>Gingiva - cytology</subject><subject>Gingiva - metabolism</subject><subject>Gum disease</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunofluorescence</subject><subject>Immunohistochemistry</subject><subject>Inflammatory diseases</subject><subject>Innate immunity</subject><subject>Kinases</subject><subject>Lysates</subject><subject>MAP kinase</subject><subject>Matrix Metalloproteinase 8 - metabolism</subject><subject>Membrane Proteins - metabolism</subject><subject>Middle Aged</subject><subject>Neutrophil collagenase</subject><subject>Periodontitis</subject><subject>Polymerase chain reaction</subject><subject>Protein expression</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Young Adult</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1rFTEQhoNY7LF64R-QgDf1Ytt8bD72stSqLRVB9DrkJLOnOeQka7Jr7b837aleCIIDw8yQZ14mvAi9ouSEtjjdFjihrGfyCVpRSUhHlBRP0YoQxjre6_4QPa91S9os1fAMHTKlxcAkWaH5MvnFhXUEDD-nArWGnHAe8Rl-F2pIM2xKSNgmjz_BbGPMU8kzhGQrYI3bk7spOQWHJygh-5zmMIf6sLAJaRN-2IhhCvMNxNBaBzHWF-hgtLHCy8d6hL69v_h6_rG7_vzh8vzsunNcS9lZ7b113o2cS06IGKxfWw09EXztmKdO694BsHEkLZlWPR2d5kqAZ5w4xY_Q8V633fx9gTqbXaj3F9gEeamG6kFx1Qut_wPtqRo416Khb_5Ct3kpqX3E0IERQQUZZKPe7ilXcq0FRjOVsLPlzlBi7l0zzTXz4FpjXz8qLusd-D_kb5sacLoHbkOEu38rmasvF3vJX-Nhohg</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Aung, W. P. P.</creator><creator>Chotjumlong, P.</creator><creator>Pata, S.</creator><creator>Montreekachon, P.</creator><creator>Supanchart, C.</creator><creator>Khongkhunthian, S.</creator><creator>Sastraruji, T.</creator><creator>Krisanaprakornkit, S.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells</title><author>Aung, W. P. P. ; Chotjumlong, P. ; Pata, S. ; Montreekachon, P. ; Supanchart, C. ; Khongkhunthian, S. ; Sastraruji, T. ; Krisanaprakornkit, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3866-a8ddacdcf33630059adba8e4053bc2d1c884cee2ff02ff28741fc8375ed230c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>A Disintegrin and Metalloproteinase 8</topic><topic>ADAM protein</topic><topic>ADAM Proteins - metabolism</topic><topic>ADAM8 protein</topic><topic>Adult</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>chronic periodontitis</topic><topic>Chronic Periodontitis - metabolism</topic><topic>Dentistry</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium</topic><topic>Fibroblasts</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fusobacterium nucleatum</topic><topic>Gene expression</topic><topic>Gingiva</topic><topic>Gingiva - cytology</topic><topic>Gingiva - metabolism</topic><topic>Gum disease</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunofluorescence</topic><topic>Immunohistochemistry</topic><topic>Inflammatory diseases</topic><topic>Innate immunity</topic><topic>Kinases</topic><topic>Lysates</topic><topic>MAP kinase</topic><topic>Matrix Metalloproteinase 8 - metabolism</topic><topic>Membrane Proteins - metabolism</topic><topic>Middle Aged</topic><topic>Neutrophil collagenase</topic><topic>Periodontitis</topic><topic>Polymerase chain reaction</topic><topic>Protein expression</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aung, W. P. P.</creatorcontrib><creatorcontrib>Chotjumlong, P.</creatorcontrib><creatorcontrib>Pata, S.</creatorcontrib><creatorcontrib>Montreekachon, P.</creatorcontrib><creatorcontrib>Supanchart, C.</creatorcontrib><creatorcontrib>Khongkhunthian, S.</creatorcontrib><creatorcontrib>Sastraruji, T.</creatorcontrib><creatorcontrib>Krisanaprakornkit, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aung, W. P. P.</au><au>Chotjumlong, P.</au><au>Pata, S.</au><au>Montreekachon, P.</au><au>Supanchart, C.</au><au>Khongkhunthian, S.</au><au>Sastraruji, T.</au><au>Krisanaprakornkit, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2017-06</date><risdate>2017</risdate><volume>52</volume><issue>3</issue><spage>582</spage><epage>593</epage><pages>582-593</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objectives
The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chronic periodontitis tissues compared with that in normal tissues. ADAM8 expression and its inductive mechanism were examined in human gingival epithelial cells (HGECs) and human gingival fibroblasts.
Material and Methods
Total RNA and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. ADAM8 mRNA and protein expression was analyzed by real‐time polymerase chain reaction, immunoblotting and immunohistochemistry. ADAM8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen‐activated protein kinase pathways was assayed by real‐time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence.
Results
ADAM8 mRNA and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues (p < 0.01). Significantly increased ADAM8 expression was detected in the gingival epithelium of chronic periodontitis tissues (p < 0.001). ADAM8 mRNA expression in HGECs, but not in human gingival fibroblasts, was significantly induced by stimulation with Fusobacterium nucleatum (p < 0.05), partially via the p44/42 mitogen‐activated protein kinase pathway. ADAM8 expression in the cell lysates and on the surface of HGECs was induced by stimulation with F. nucleatum.
Conclusion
ADAM8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of ADAM8 expression in F. nucleatum‐stimulated HGECs, suggesting a possible role of ADAM8 in innate immunity of periodontal tissue.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27859260</pmid><doi>10.1111/jre.12426</doi><tpages>12</tpages></addata></record> |
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| language | eng |
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| subjects | A Disintegrin and Metalloproteinase 8 ADAM protein ADAM Proteins - metabolism ADAM8 protein Adult Blotting, Western Case-Control Studies chronic periodontitis Chronic Periodontitis - metabolism Dentistry Epithelial cells Epithelial Cells - metabolism Epithelium Fibroblasts Flow Cytometry Fluorescent Antibody Technique Fusobacterium nucleatum Gene expression Gingiva Gingiva - cytology Gingiva - metabolism Gum disease Humans Immunoblotting Immunofluorescence Immunohistochemistry Inflammatory diseases Innate immunity Kinases Lysates MAP kinase Matrix Metalloproteinase 8 - metabolism Membrane Proteins - metabolism Middle Aged Neutrophil collagenase Periodontitis Polymerase chain reaction Protein expression Protein kinase Proteins Real-Time Polymerase Chain Reaction Young Adult |
| title | Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells |
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