Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans
Aims/hypotheses Ceramides and other sphingolipids comprise a family of lipid molecules that accumulate in skeletal muscle and promote insulin resistance. Chronic endurance exercise training decreases muscle ceramides and other sphingolipids, but less is known about the effects of a single bout of ex...
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| Veröffentlicht in: | Diabetologia 2016-04, Vol.59 (4), p.785-798 |
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| creator | Bergman, Bryan C. Brozinick, Joseph T. Strauss, Allison Bacon, Samantha Kerege, Anna Bui, Hai Hoang Sanders, Phil Siddall, Parker Wei, Tao Thomas, Melissa K. Kuo, Ming Shang Perreault, Leigh |
| description | Aims/hypotheses
Ceramides and other sphingolipids comprise a family of lipid molecules that accumulate in skeletal muscle and promote insulin resistance. Chronic endurance exercise training decreases muscle ceramides and other sphingolipids, but less is known about the effects of a single bout of exercise.
Methods
We measured basal relationships and the effect of acute exercise (1.5 h at 50%
V
.
O
2
max
) and recovery on muscle sphingolipid content in obese volunteers, endurance trained athletes and individuals with type 2 diabetes.
Results
Muscle C18:0 ceramide (
p =
0.029), dihydroceramide (
p =
0.06) and glucosylceramide (
p =
0.03) species were inversely related to insulin sensitivity without differences in total ceramide, dihydroceramide, and glucosylceramide concentration. Muscle C18:0 dihydroceramide correlated with markers of muscle inflammation (
p =
0.04). Transcription of genes encoding sphingolipid synthesis enzymes was higher in athletes, suggesting an increased capacity for sphingolipid synthesis. The total concentration of muscle ceramides and sphingolipids increased during exercise and then decreased after recovery, during which time ceramide levels reduced to significantly below basal levels.
Conclusions/interpretation
These data suggest ceramide and other sphingolipids containing stearate (18:0) are uniquely related to insulin resistance in skeletal muscle. Recovery from an exercise bout decreased muscle ceramide concentration; this may represent a mechanism promoting the insulin-sensitising effects of acute exercise. |
| doi_str_mv | 10.1007/s00125-015-3850-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1897371381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1897371381</sourcerecordid><originalsourceid>FETCH-LOGICAL-c518t-68cc45736d03a701af9cca6338d99b5ea75492d63f9c346dc145f7526fdaa7ab3</originalsourceid><addsrcrecordid>eNqFkU1rFTEUhoNY7G31B7iRgBs3ozmTycd0Jxe1QqWbFtyF3CTTpszNjDk30PvvzTBVRBBXISfPe06Sh5DXwN4DY-oDMgataBiIhmvBmuMzsoGOtw3rWv2cbJbjBrT8fkrOEB8YY1x08gU5baXivQaxIfZbQTcGivN9THfTGOfokfqS647mgAdqk6fhMWQXMVxQS7egLxjFeJfsoeRAhynTmLCMMS2BiAebXKglel_2NuFLcjLYEcOrp_Wc3H7-dLO9bK6uv3zdfrxqnAB9aKR2rhOKS8-4VQzs0DtnJefa9_1OBKtE17de8lrnnfQOOjEo0crBW6vsjp-Td2vfOU8_Sr252Ud0YRxtClNBA7pXXAHX8H9UKabr9whd0bd_oQ9Tyak-ZKV4D6KvFKyUyxNiDoOZc9zbfDTAzKLKrKpMVWUWVeZYM2-eOpfdPvjfiV9uKtCuAM6LjZD_GP3Prj8B6QeeRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770839159</pqid></control><display><type>article</type><title>Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Bergman, Bryan C. ; Brozinick, Joseph T. ; Strauss, Allison ; Bacon, Samantha ; Kerege, Anna ; Bui, Hai Hoang ; Sanders, Phil ; Siddall, Parker ; Wei, Tao ; Thomas, Melissa K. ; Kuo, Ming Shang ; Perreault, Leigh</creator><creatorcontrib>Bergman, Bryan C. ; Brozinick, Joseph T. ; Strauss, Allison ; Bacon, Samantha ; Kerege, Anna ; Bui, Hai Hoang ; Sanders, Phil ; Siddall, Parker ; Wei, Tao ; Thomas, Melissa K. ; Kuo, Ming Shang ; Perreault, Leigh</creatorcontrib><description>Aims/hypotheses
Ceramides and other sphingolipids comprise a family of lipid molecules that accumulate in skeletal muscle and promote insulin resistance. Chronic endurance exercise training decreases muscle ceramides and other sphingolipids, but less is known about the effects of a single bout of exercise.
Methods
We measured basal relationships and the effect of acute exercise (1.5 h at 50%
V
.
O
2
max
) and recovery on muscle sphingolipid content in obese volunteers, endurance trained athletes and individuals with type 2 diabetes.
Results
Muscle C18:0 ceramide (
p =
0.029), dihydroceramide (
p =
0.06) and glucosylceramide (
p =
0.03) species were inversely related to insulin sensitivity without differences in total ceramide, dihydroceramide, and glucosylceramide concentration. Muscle C18:0 dihydroceramide correlated with markers of muscle inflammation (
p =
0.04). Transcription of genes encoding sphingolipid synthesis enzymes was higher in athletes, suggesting an increased capacity for sphingolipid synthesis. The total concentration of muscle ceramides and sphingolipids increased during exercise and then decreased after recovery, during which time ceramide levels reduced to significantly below basal levels.
Conclusions/interpretation
These data suggest ceramide and other sphingolipids containing stearate (18:0) are uniquely related to insulin resistance in skeletal muscle. Recovery from an exercise bout decreased muscle ceramide concentration; this may represent a mechanism promoting the insulin-sensitising effects of acute exercise.</description><identifier>ISSN: 0012-186X</identifier><identifier>EISSN: 1432-0428</identifier><identifier>DOI: 10.1007/s00125-015-3850-y</identifier><identifier>PMID: 26739815</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Blotting, Western ; Ceramides - metabolism ; Diabetes ; Exercise ; Exercise - physiology ; Fitness training programs ; Human Physiology ; Humans ; Insulin resistance ; Insulin Resistance - physiology ; Internal Medicine ; Kinases ; Lipids ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Muscle, Skeletal - metabolism ; Musculoskeletal system ; Physical fitness ; Rest - physiology ; Sphingolipids - metabolism</subject><ispartof>Diabetologia, 2016-04, Vol.59 (4), p.785-798</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-68cc45736d03a701af9cca6338d99b5ea75492d63f9c346dc145f7526fdaa7ab3</citedby><cites>FETCH-LOGICAL-c518t-68cc45736d03a701af9cca6338d99b5ea75492d63f9c346dc145f7526fdaa7ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00125-015-3850-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00125-015-3850-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26739815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bergman, Bryan C.</creatorcontrib><creatorcontrib>Brozinick, Joseph T.</creatorcontrib><creatorcontrib>Strauss, Allison</creatorcontrib><creatorcontrib>Bacon, Samantha</creatorcontrib><creatorcontrib>Kerege, Anna</creatorcontrib><creatorcontrib>Bui, Hai Hoang</creatorcontrib><creatorcontrib>Sanders, Phil</creatorcontrib><creatorcontrib>Siddall, Parker</creatorcontrib><creatorcontrib>Wei, Tao</creatorcontrib><creatorcontrib>Thomas, Melissa K.</creatorcontrib><creatorcontrib>Kuo, Ming Shang</creatorcontrib><creatorcontrib>Perreault, Leigh</creatorcontrib><title>Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans</title><title>Diabetologia</title><addtitle>Diabetologia</addtitle><addtitle>Diabetologia</addtitle><description>Aims/hypotheses
Ceramides and other sphingolipids comprise a family of lipid molecules that accumulate in skeletal muscle and promote insulin resistance. Chronic endurance exercise training decreases muscle ceramides and other sphingolipids, but less is known about the effects of a single bout of exercise.
Methods
We measured basal relationships and the effect of acute exercise (1.5 h at 50%
V
.
O
2
max
) and recovery on muscle sphingolipid content in obese volunteers, endurance trained athletes and individuals with type 2 diabetes.
Results
Muscle C18:0 ceramide (
p =
0.029), dihydroceramide (
p =
0.06) and glucosylceramide (
p =
0.03) species were inversely related to insulin sensitivity without differences in total ceramide, dihydroceramide, and glucosylceramide concentration. Muscle C18:0 dihydroceramide correlated with markers of muscle inflammation (
p =
0.04). Transcription of genes encoding sphingolipid synthesis enzymes was higher in athletes, suggesting an increased capacity for sphingolipid synthesis. The total concentration of muscle ceramides and sphingolipids increased during exercise and then decreased after recovery, during which time ceramide levels reduced to significantly below basal levels.
Conclusions/interpretation
These data suggest ceramide and other sphingolipids containing stearate (18:0) are uniquely related to insulin resistance in skeletal muscle. Recovery from an exercise bout decreased muscle ceramide concentration; this may represent a mechanism promoting the insulin-sensitising effects of acute exercise.</description><subject>Adult</subject><subject>Blotting, Western</subject><subject>Ceramides - metabolism</subject><subject>Diabetes</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Fitness training programs</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Insulin resistance</subject><subject>Insulin Resistance - physiology</subject><subject>Internal Medicine</subject><subject>Kinases</subject><subject>Lipids</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Musculoskeletal system</subject><subject>Physical fitness</subject><subject>Rest - physiology</subject><subject>Sphingolipids - metabolism</subject><issn>0012-186X</issn><issn>1432-0428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkU1rFTEUhoNY7G31B7iRgBs3ozmTycd0Jxe1QqWbFtyF3CTTpszNjDk30PvvzTBVRBBXISfPe06Sh5DXwN4DY-oDMgataBiIhmvBmuMzsoGOtw3rWv2cbJbjBrT8fkrOEB8YY1x08gU5baXivQaxIfZbQTcGivN9THfTGOfokfqS647mgAdqk6fhMWQXMVxQS7egLxjFeJfsoeRAhynTmLCMMS2BiAebXKglel_2NuFLcjLYEcOrp_Wc3H7-dLO9bK6uv3zdfrxqnAB9aKR2rhOKS8-4VQzs0DtnJefa9_1OBKtE17de8lrnnfQOOjEo0crBW6vsjp-Td2vfOU8_Sr252Ud0YRxtClNBA7pXXAHX8H9UKabr9whd0bd_oQ9Tyak-ZKV4D6KvFKyUyxNiDoOZc9zbfDTAzKLKrKpMVWUWVeZYM2-eOpfdPvjfiV9uKtCuAM6LjZD_GP3Prj8B6QeeRQ</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Bergman, Bryan C.</creator><creator>Brozinick, Joseph T.</creator><creator>Strauss, Allison</creator><creator>Bacon, Samantha</creator><creator>Kerege, Anna</creator><creator>Bui, Hai Hoang</creator><creator>Sanders, Phil</creator><creator>Siddall, Parker</creator><creator>Wei, Tao</creator><creator>Thomas, Melissa K.</creator><creator>Kuo, Ming Shang</creator><creator>Perreault, Leigh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TS</scope></search><sort><creationdate>20160401</creationdate><title>Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans</title><author>Bergman, Bryan C. ; Brozinick, Joseph T. ; Strauss, Allison ; Bacon, Samantha ; Kerege, Anna ; Bui, Hai Hoang ; Sanders, Phil ; Siddall, Parker ; Wei, Tao ; Thomas, Melissa K. ; Kuo, Ming Shang ; Perreault, Leigh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-68cc45736d03a701af9cca6338d99b5ea75492d63f9c346dc145f7526fdaa7ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Blotting, Western</topic><topic>Ceramides - metabolism</topic><topic>Diabetes</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Fitness training programs</topic><topic>Human Physiology</topic><topic>Humans</topic><topic>Insulin resistance</topic><topic>Insulin Resistance - physiology</topic><topic>Internal Medicine</topic><topic>Kinases</topic><topic>Lipids</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Musculoskeletal system</topic><topic>Physical fitness</topic><topic>Rest - physiology</topic><topic>Sphingolipids - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bergman, Bryan C.</creatorcontrib><creatorcontrib>Brozinick, Joseph T.</creatorcontrib><creatorcontrib>Strauss, Allison</creatorcontrib><creatorcontrib>Bacon, Samantha</creatorcontrib><creatorcontrib>Kerege, Anna</creatorcontrib><creatorcontrib>Bui, Hai Hoang</creatorcontrib><creatorcontrib>Sanders, Phil</creatorcontrib><creatorcontrib>Siddall, Parker</creatorcontrib><creatorcontrib>Wei, Tao</creatorcontrib><creatorcontrib>Thomas, Melissa K.</creatorcontrib><creatorcontrib>Kuo, Ming Shang</creatorcontrib><creatorcontrib>Perreault, Leigh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Physical Education Index</collection><jtitle>Diabetologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bergman, Bryan C.</au><au>Brozinick, Joseph T.</au><au>Strauss, Allison</au><au>Bacon, Samantha</au><au>Kerege, Anna</au><au>Bui, Hai Hoang</au><au>Sanders, Phil</au><au>Siddall, Parker</au><au>Wei, Tao</au><au>Thomas, Melissa K.</au><au>Kuo, Ming Shang</au><au>Perreault, Leigh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans</atitle><jtitle>Diabetologia</jtitle><stitle>Diabetologia</stitle><addtitle>Diabetologia</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>59</volume><issue>4</issue><spage>785</spage><epage>798</epage><pages>785-798</pages><issn>0012-186X</issn><eissn>1432-0428</eissn><abstract>Aims/hypotheses
Ceramides and other sphingolipids comprise a family of lipid molecules that accumulate in skeletal muscle and promote insulin resistance. Chronic endurance exercise training decreases muscle ceramides and other sphingolipids, but less is known about the effects of a single bout of exercise.
Methods
We measured basal relationships and the effect of acute exercise (1.5 h at 50%
V
.
O
2
max
) and recovery on muscle sphingolipid content in obese volunteers, endurance trained athletes and individuals with type 2 diabetes.
Results
Muscle C18:0 ceramide (
p =
0.029), dihydroceramide (
p =
0.06) and glucosylceramide (
p =
0.03) species were inversely related to insulin sensitivity without differences in total ceramide, dihydroceramide, and glucosylceramide concentration. Muscle C18:0 dihydroceramide correlated with markers of muscle inflammation (
p =
0.04). Transcription of genes encoding sphingolipid synthesis enzymes was higher in athletes, suggesting an increased capacity for sphingolipid synthesis. The total concentration of muscle ceramides and sphingolipids increased during exercise and then decreased after recovery, during which time ceramide levels reduced to significantly below basal levels.
Conclusions/interpretation
These data suggest ceramide and other sphingolipids containing stearate (18:0) are uniquely related to insulin resistance in skeletal muscle. Recovery from an exercise bout decreased muscle ceramide concentration; this may represent a mechanism promoting the insulin-sensitising effects of acute exercise.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26739815</pmid><doi>10.1007/s00125-015-3850-y</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-186X |
| ispartof | Diabetologia, 2016-04, Vol.59 (4), p.785-798 |
| issn | 0012-186X 1432-0428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1897371381 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adult Blotting, Western Ceramides - metabolism Diabetes Exercise Exercise - physiology Fitness training programs Human Physiology Humans Insulin resistance Insulin Resistance - physiology Internal Medicine Kinases Lipids Medicine Medicine & Public Health Metabolic Diseases Muscle, Skeletal - metabolism Musculoskeletal system Physical fitness Rest - physiology Sphingolipids - metabolism |
| title | Muscle sphingolipids during rest and exercise: a C18:0 signature for insulin resistance in humans |
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