Fluorescent quantitative PCR detection of Mycobacterium tuberculosis in tissue sections from granulomatous lesions retrieved using EDTA

AimsThis study aimed to use EDTA to retrieve paraffin-embedded tissue sections of inflammatory granulomatous lesions and increase the detection rate of tuberculosis (TB)/non-tuberculous mycobacteria. Due to the influence of chemical reagents during the fixation process, the amplification of fluoresc...

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Veröffentlicht in:	Journal of clinical pathology 2017-05, Vol.70 (5), p.390-394
Hauptverfasser:	Wang, Xuzhou, Xie, Feilai, Zheng, Qiaoling, Qi, Xingfeng, Li, Min, Zhou, Xiaoling, Zheng, Zhiyong
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Biopsy - methods DNA, Bacterial - isolation & purification Edetic Acid Female Fluorescence Granuloma - diagnosis Granuloma - microbiology Granuloma - pathology Humans Lung - microbiology Lung - pathology Male Middle Aged Mycobacterium Infections, Nontuberculous - diagnosis Mycobacterium Infections, Nontuberculous - microbiology Mycobacterium Infections, Nontuberculous - pathology Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Nontuberculous Mycobacteria - genetics Nontuberculous Mycobacteria - isolation & purification Paraffin Embedding Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Tuberculosis - diagnosis Tuberculosis - microbiology Tuberculosis - pathology
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