Phyllanthus niruri leaves aqueous extract improves kidney functions, ameliorates kidney oxidative stress, inflammation, fibrosis and apoptosis and enhances kidney cell proliferation in adult male rats with diabetes mellitus
Phylanthus niruri has been used to treat ailments related to the urogenital organs. In this study, this herb was hypothesized to help to ameliorate kidney disease in diabetes mellitus (DM). To investigate P. niruri leaves aqueous extract (PN) effects on kidney functions, histopathological changes an...
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| Veröffentlicht in: | Journal of ethnopharmacology 2017-06, Vol.205, p.123-137 |
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| creator | Giribabu, Nelli Karim, Kamarulzaman Kilari, Eswar Kumar Salleh, Naguib |
| description | Phylanthus niruri has been used to treat ailments related to the urogenital organs. In this study, this herb was hypothesized to help to ameliorate kidney disease in diabetes mellitus (DM).
To investigate P. niruri leaves aqueous extract (PN) effects on kidney functions, histopathological changes and levels of oxidative stress, inflammation, fibrosis, apoptosis and proliferation in DM.
PN was orally administered to streptozotocin-nicotinamide-induced male diabetic rats for 28 days. At the end of the treatment, fasting blood glucose (FBG) and kidney functions were measured. Kidney somatic index, histopathological changes and levels of RAGE, Nrf2, oxidative stress markers (TBARS, SOD, CAT and GPx), inflammatory markers (NFkβ-p65, Ikk-β, TNF-α, IL-1β and IL-6), apoptosis markers (caspase-3, caspase-9 and Bax), fibrosis markers (TGF-β1, VEGF and FGF-1) and proliferative markers (PCNA and Ki-67) were determined by biochemical assays, qPCR, Western blotting, immunohistochemistry or immunofluorescence.
Administration of PN helps to maintain near normal FBG, creatinine clearance (CCr), blood urea nitrogen (BUN), BUN/Cr ratio, serum electrolytes, uric acid and urine protein levels in DM. Decreased RAGE, TBARS and increased Nrf2, SOD-1, CAT and GPx-1 were observed in PN-treated diabetic rat kidneys. Expression of inflammatory, fibrosis and apoptosis markers in the kidney reduced but expression of proliferative markers increased following PN treatment. Lesser histopathological changes were observed in the kidney of PN-treated diabetic rats.
PN helps to preserve near normal kidney function and prevents histopathological changes via ameliorating oxidative stress, inflammation, fibrosis and apoptosis while enhancing proliferation of the kidney in DM.
[Display omitted] |
| doi_str_mv | 10.1016/j.jep.2017.05.002 |
| format | Article |
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To investigate P. niruri leaves aqueous extract (PN) effects on kidney functions, histopathological changes and levels of oxidative stress, inflammation, fibrosis, apoptosis and proliferation in DM.
PN was orally administered to streptozotocin-nicotinamide-induced male diabetic rats for 28 days. At the end of the treatment, fasting blood glucose (FBG) and kidney functions were measured. Kidney somatic index, histopathological changes and levels of RAGE, Nrf2, oxidative stress markers (TBARS, SOD, CAT and GPx), inflammatory markers (NFkβ-p65, Ikk-β, TNF-α, IL-1β and IL-6), apoptosis markers (caspase-3, caspase-9 and Bax), fibrosis markers (TGF-β1, VEGF and FGF-1) and proliferative markers (PCNA and Ki-67) were determined by biochemical assays, qPCR, Western blotting, immunohistochemistry or immunofluorescence.
Administration of PN helps to maintain near normal FBG, creatinine clearance (CCr), blood urea nitrogen (BUN), BUN/Cr ratio, serum electrolytes, uric acid and urine protein levels in DM. Decreased RAGE, TBARS and increased Nrf2, SOD-1, CAT and GPx-1 were observed in PN-treated diabetic rat kidneys. Expression of inflammatory, fibrosis and apoptosis markers in the kidney reduced but expression of proliferative markers increased following PN treatment. Lesser histopathological changes were observed in the kidney of PN-treated diabetic rats.
PN helps to preserve near normal kidney function and prevents histopathological changes via ameliorating oxidative stress, inflammation, fibrosis and apoptosis while enhancing proliferation of the kidney in DM.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2017.05.002</identifier><identifier>PMID: 28483637</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Diabetes Mellitus, Experimental - complications ; Diabetic Nephropathies - prevention & control ; Fibrosis ; Fibrosis - drug therapy ; Inflammation ; Inflammation - drug therapy ; Kidney - cytology ; Kidney - drug effects ; Kidney - physiology ; Male ; Phylanthus niruri ; Phyllanthus - chemistry ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Proliferation ; Rats ; Rats, Wistar ; Renal function ; Renal oxidative stress</subject><ispartof>Journal of ethnopharmacology, 2017-06, Vol.205, p.123-137</ispartof><rights>2017 Elsevier Ireland Ltd</rights><rights>Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-594f23ef301ba08b5313bbff25c1b888302b18919fc19dc4bd004d73ef98fc543</citedby><cites>FETCH-LOGICAL-c353t-594f23ef301ba08b5313bbff25c1b888302b18919fc19dc4bd004d73ef98fc543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S037887411630993X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28483637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giribabu, Nelli</creatorcontrib><creatorcontrib>Karim, Kamarulzaman</creatorcontrib><creatorcontrib>Kilari, Eswar Kumar</creatorcontrib><creatorcontrib>Salleh, Naguib</creatorcontrib><title>Phyllanthus niruri leaves aqueous extract improves kidney functions, ameliorates kidney oxidative stress, inflammation, fibrosis and apoptosis and enhances kidney cell proliferation in adult male rats with diabetes mellitus</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Phylanthus niruri has been used to treat ailments related to the urogenital organs. In this study, this herb was hypothesized to help to ameliorate kidney disease in diabetes mellitus (DM).
To investigate P. niruri leaves aqueous extract (PN) effects on kidney functions, histopathological changes and levels of oxidative stress, inflammation, fibrosis, apoptosis and proliferation in DM.
PN was orally administered to streptozotocin-nicotinamide-induced male diabetic rats for 28 days. At the end of the treatment, fasting blood glucose (FBG) and kidney functions were measured. Kidney somatic index, histopathological changes and levels of RAGE, Nrf2, oxidative stress markers (TBARS, SOD, CAT and GPx), inflammatory markers (NFkβ-p65, Ikk-β, TNF-α, IL-1β and IL-6), apoptosis markers (caspase-3, caspase-9 and Bax), fibrosis markers (TGF-β1, VEGF and FGF-1) and proliferative markers (PCNA and Ki-67) were determined by biochemical assays, qPCR, Western blotting, immunohistochemistry or immunofluorescence.
Administration of PN helps to maintain near normal FBG, creatinine clearance (CCr), blood urea nitrogen (BUN), BUN/Cr ratio, serum electrolytes, uric acid and urine protein levels in DM. Decreased RAGE, TBARS and increased Nrf2, SOD-1, CAT and GPx-1 were observed in PN-treated diabetic rat kidneys. Expression of inflammatory, fibrosis and apoptosis markers in the kidney reduced but expression of proliferative markers increased following PN treatment. Lesser histopathological changes were observed in the kidney of PN-treated diabetic rats.
PN helps to preserve near normal kidney function and prevents histopathological changes via ameliorating oxidative stress, inflammation, fibrosis and apoptosis while enhancing proliferation of the kidney in DM.
[Display omitted]</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetic Nephropathies - prevention & control</subject><subject>Fibrosis</subject><subject>Fibrosis - drug therapy</subject><subject>Inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Kidney - cytology</subject><subject>Kidney - drug effects</subject><subject>Kidney - physiology</subject><subject>Male</subject><subject>Phylanthus niruri</subject><subject>Phyllanthus - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Proliferation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Renal function</subject><subject>Renal oxidative stress</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQtRCIbhd-ABfkI4cm2HGyccQJVUCRKsEBzpZjj7WzOB_YztL9tfwVHLa0N-SD5Zl5b57fI-QVZyVnfPf2UB5gLivG25I1JWPVE7Lhsq2KtmnFU7JhopWFbGt-QS5jPDDGWl6z5-SikrUUO9FuyO-v-5P3ekz7JdIRwxKQetBHiFT_XGDKVbhLQZtEcZjDtDZ-oB3hRN0ymoTTGK-oHsDjFHR67E53aHXCI9CYAsQ8hKPzehj0irmiDvswRcxrRkv1PM3p4QXjXo_mkcqA9zTv9ugg_IVnLqrt4hMdtAeai5H-wrSnFnUPq4osyGNa4gvyzGkf4eX9vSXfP374dn1T3H759Pn6_W1hRCNS0XS1qwQ4wXivmewbwUXfO1c1hvdSSsGqnsuOd87wzpq6t4zVts2ITjrT1GJL3px5s87sW0xqwLgK1-NqosrgnezEeraEn0dNNiAGcGoOOOhwUpypNVd1UDlXteaqWKNyrhnz-p5-6QewD4h_QeaBd-cByJ88IgQVDUJ20WIAk5Sd8D_0fwAzj7tY</recordid><startdate>20170609</startdate><enddate>20170609</enddate><creator>Giribabu, Nelli</creator><creator>Karim, Kamarulzaman</creator><creator>Kilari, Eswar Kumar</creator><creator>Salleh, Naguib</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170609</creationdate><title>Phyllanthus niruri leaves aqueous extract improves kidney functions, ameliorates kidney oxidative stress, inflammation, fibrosis and apoptosis and enhances kidney cell proliferation in adult male rats with diabetes mellitus</title><author>Giribabu, Nelli ; Karim, Kamarulzaman ; Kilari, Eswar Kumar ; Salleh, Naguib</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-594f23ef301ba08b5313bbff25c1b888302b18919fc19dc4bd004d73ef98fc543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetic Nephropathies - prevention & control</topic><topic>Fibrosis</topic><topic>Fibrosis - drug therapy</topic><topic>Inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Kidney - cytology</topic><topic>Kidney - drug effects</topic><topic>Kidney - physiology</topic><topic>Male</topic><topic>Phylanthus niruri</topic><topic>Phyllanthus - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Proliferation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Renal function</topic><topic>Renal oxidative stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giribabu, Nelli</creatorcontrib><creatorcontrib>Karim, Kamarulzaman</creatorcontrib><creatorcontrib>Kilari, Eswar Kumar</creatorcontrib><creatorcontrib>Salleh, Naguib</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giribabu, Nelli</au><au>Karim, Kamarulzaman</au><au>Kilari, Eswar Kumar</au><au>Salleh, Naguib</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phyllanthus niruri leaves aqueous extract improves kidney functions, ameliorates kidney oxidative stress, inflammation, fibrosis and apoptosis and enhances kidney cell proliferation in adult male rats with diabetes mellitus</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2017-06-09</date><risdate>2017</risdate><volume>205</volume><spage>123</spage><epage>137</epage><pages>123-137</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Phylanthus niruri has been used to treat ailments related to the urogenital organs. In this study, this herb was hypothesized to help to ameliorate kidney disease in diabetes mellitus (DM).
To investigate P. niruri leaves aqueous extract (PN) effects on kidney functions, histopathological changes and levels of oxidative stress, inflammation, fibrosis, apoptosis and proliferation in DM.
PN was orally administered to streptozotocin-nicotinamide-induced male diabetic rats for 28 days. At the end of the treatment, fasting blood glucose (FBG) and kidney functions were measured. Kidney somatic index, histopathological changes and levels of RAGE, Nrf2, oxidative stress markers (TBARS, SOD, CAT and GPx), inflammatory markers (NFkβ-p65, Ikk-β, TNF-α, IL-1β and IL-6), apoptosis markers (caspase-3, caspase-9 and Bax), fibrosis markers (TGF-β1, VEGF and FGF-1) and proliferative markers (PCNA and Ki-67) were determined by biochemical assays, qPCR, Western blotting, immunohistochemistry or immunofluorescence.
Administration of PN helps to maintain near normal FBG, creatinine clearance (CCr), blood urea nitrogen (BUN), BUN/Cr ratio, serum electrolytes, uric acid and urine protein levels in DM. Decreased RAGE, TBARS and increased Nrf2, SOD-1, CAT and GPx-1 were observed in PN-treated diabetic rat kidneys. Expression of inflammatory, fibrosis and apoptosis markers in the kidney reduced but expression of proliferative markers increased following PN treatment. Lesser histopathological changes were observed in the kidney of PN-treated diabetic rats.
PN helps to preserve near normal kidney function and prevents histopathological changes via ameliorating oxidative stress, inflammation, fibrosis and apoptosis while enhancing proliferation of the kidney in DM.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>28483637</pmid><doi>10.1016/j.jep.2017.05.002</doi><tpages>15</tpages></addata></record> |
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| ispartof | Journal of ethnopharmacology, 2017-06, Vol.205, p.123-137 |
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| subjects | Animals Apoptosis Apoptosis - drug effects Diabetes Mellitus, Experimental - complications Diabetic Nephropathies - prevention & control Fibrosis Fibrosis - drug therapy Inflammation Inflammation - drug therapy Kidney - cytology Kidney - drug effects Kidney - physiology Male Phylanthus niruri Phyllanthus - chemistry Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Proliferation Rats Rats, Wistar Renal function Renal oxidative stress |
| title | Phyllanthus niruri leaves aqueous extract improves kidney functions, ameliorates kidney oxidative stress, inflammation, fibrosis and apoptosis and enhances kidney cell proliferation in adult male rats with diabetes mellitus |
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