In vivo two-photon imaging of retina in rabbits and rats
The purpose of this study was to evaluate the retina using near-infrared (NIR) two-photon scanning laser ophthalmoscopy. New Zealand white rabbits, albino rats, and brown Norway rats were used in this study. An autofluorescence image of the retina, including the retinal cells and its associated vasc...
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| Veröffentlicht in: | Experimental eye research 2018-01, Vol.166, p.40-48 |
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| creator | Jayabalan, Gopal Swamy Wu, Yi-Kai Bille, Josef F. Kim, Samuel Mao, Xiao Wen Gimbel, Howard V. Rauser, Michael E. Fan, Joseph T. |
| description | The purpose of this study was to evaluate the retina using near-infrared (NIR) two-photon scanning laser ophthalmoscopy. New Zealand white rabbits, albino rats, and brown Norway rats were used in this study. An autofluorescence image of the retina, including the retinal cells and its associated vasculatures was obtained by a real-time scan using the ophthalmoscope. Furthermore, the retinal vessels, nerve fiber layers and the non-pigmented retina were recorded with two-photon fluorescein angiography (FA); and the choroidal vasculatures were recorded using two-photon indocyanine green angiography (ICGA). Two-photon ICGA was achieved by exciting a second singlet state at ∼398 nm. Simultaneous two-photon FA and two-photon ICGA were performed to characterize the retinal and choroidal vessels with a single injection. The minimum laser power threshold required to elicit two-photon fluorescence was determined. The two-photon ophthalmoscope could serve as a promising tool to detect and monitor the disease progression in animal models. Moreover, these high-resolution images of retinal and choroidal vessels can be acquired in a real-time scan with a single light source, requiring no additional filters for FA or ICGA. The combination of FA and ICGA using the two-photon ophthalmoscope will help researchers to characterize the retinal diseases in animal models, and also to classify the types (classic, occult or mixed) of choroidal neovascularization (CNV) in macular degeneration. Furthermore, the prototype can be adapted to image the retina of rodents and rabbits.
•Two-photon excited fluorescence imaging at 780 nm allows multimodal fundus imaging with high quality and contrast.•Two-photon fundus imaging with a 30° field of view is significant.•Demonstrated two-photon fluorescein angiography (FA) and two-photon indocyanine green angiography (ICGA).•Investigated the two-photon excitation and emission of second singlet state of indocyanine green dye.•Simultaneous two-photon FA and ICGA with a single light source were achieved requiring no additional filters. |
| doi_str_mv | 10.1016/j.exer.2017.04.009 |
| format | Article |
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•Two-photon excited fluorescence imaging at 780 nm allows multimodal fundus imaging with high quality and contrast.•Two-photon fundus imaging with a 30° field of view is significant.•Demonstrated two-photon fluorescein angiography (FA) and two-photon indocyanine green angiography (ICGA).•Investigated the two-photon excitation and emission of second singlet state of indocyanine green dye.•Simultaneous two-photon FA and ICGA with a single light source were achieved requiring no additional filters.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2017.04.009</identifier><identifier>PMID: 28483661</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animal models ; Femtosecond laser ; Fluorescein angiography ; Indocyanine green angiography ; Nonlinear optics ; Retinal imaging ; Two-photon ophthalmoscope</subject><ispartof>Experimental eye research, 2018-01, Vol.166, p.40-48</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-bfe1e77d8dbc0f69ac716752fd555c2809508f12d7dd19984951f565469e306b3</citedby><cites>FETCH-LOGICAL-c422t-bfe1e77d8dbc0f69ac716752fd555c2809508f12d7dd19984951f565469e306b3</cites><orcidid>0000-0002-0494-7459 ; 0000-0002-5648-9664</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exer.2017.04.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28483661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jayabalan, Gopal Swamy</creatorcontrib><creatorcontrib>Wu, Yi-Kai</creatorcontrib><creatorcontrib>Bille, Josef F.</creatorcontrib><creatorcontrib>Kim, Samuel</creatorcontrib><creatorcontrib>Mao, Xiao Wen</creatorcontrib><creatorcontrib>Gimbel, Howard V.</creatorcontrib><creatorcontrib>Rauser, Michael E.</creatorcontrib><creatorcontrib>Fan, Joseph T.</creatorcontrib><title>In vivo two-photon imaging of retina in rabbits and rats</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The purpose of this study was to evaluate the retina using near-infrared (NIR) two-photon scanning laser ophthalmoscopy. New Zealand white rabbits, albino rats, and brown Norway rats were used in this study. An autofluorescence image of the retina, including the retinal cells and its associated vasculatures was obtained by a real-time scan using the ophthalmoscope. Furthermore, the retinal vessels, nerve fiber layers and the non-pigmented retina were recorded with two-photon fluorescein angiography (FA); and the choroidal vasculatures were recorded using two-photon indocyanine green angiography (ICGA). Two-photon ICGA was achieved by exciting a second singlet state at ∼398 nm. Simultaneous two-photon FA and two-photon ICGA were performed to characterize the retinal and choroidal vessels with a single injection. The minimum laser power threshold required to elicit two-photon fluorescence was determined. The two-photon ophthalmoscope could serve as a promising tool to detect and monitor the disease progression in animal models. Moreover, these high-resolution images of retinal and choroidal vessels can be acquired in a real-time scan with a single light source, requiring no additional filters for FA or ICGA. The combination of FA and ICGA using the two-photon ophthalmoscope will help researchers to characterize the retinal diseases in animal models, and also to classify the types (classic, occult or mixed) of choroidal neovascularization (CNV) in macular degeneration. Furthermore, the prototype can be adapted to image the retina of rodents and rabbits.
•Two-photon excited fluorescence imaging at 780 nm allows multimodal fundus imaging with high quality and contrast.•Two-photon fundus imaging with a 30° field of view is significant.•Demonstrated two-photon fluorescein angiography (FA) and two-photon indocyanine green angiography (ICGA).•Investigated the two-photon excitation and emission of second singlet state of indocyanine green dye.•Simultaneous two-photon FA and ICGA with a single light source were achieved requiring no additional filters.</description><subject>Animal models</subject><subject>Femtosecond laser</subject><subject>Fluorescein angiography</subject><subject>Indocyanine green angiography</subject><subject>Nonlinear optics</subject><subject>Retinal imaging</subject><subject>Two-photon ophthalmoscope</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kM1KAzEUhYMotv68gAuZpZsZbzJJJgE3UvyDghtdh5kkoyltUpNp1bfxWXwyU1pdurqXwzkHzofQGYYKA-aXs8p-2FgRwE0FtAKQe2iMQfISAJp9NAbAtKSiZiN0lNIsqzVt6CEaEZFVzvEYyQf__bV261AM76FcvoYh-MIt2hfnX4rQF9EOzreF80Vsu84NqWi9yf-QTtBB386TPd3dY_R8e_M0uS-nj3cPk-tpqSkhQ9n1FtumMcJ0GnouW91g3jDSG8aYJgIkA9FjYhpjsJSCSoZ7xhnl0tbAu_oYXWx7lzG8rWwa1MIlbefz1tuwSgoLyYWsBafZSrZWHUNK0fZqGfOW-KkwqA0yNVMbZGqDTAFVGVkOne_6V93Cmr_IL6NsuNoabF65djmetLNeW-Oi1YMywf3X_wNVJXw9</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Jayabalan, Gopal Swamy</creator><creator>Wu, Yi-Kai</creator><creator>Bille, Josef F.</creator><creator>Kim, Samuel</creator><creator>Mao, Xiao Wen</creator><creator>Gimbel, Howard V.</creator><creator>Rauser, Michael E.</creator><creator>Fan, Joseph T.</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0494-7459</orcidid><orcidid>https://orcid.org/0000-0002-5648-9664</orcidid></search><sort><creationdate>20180101</creationdate><title>In vivo two-photon imaging of retina in rabbits and rats</title><author>Jayabalan, Gopal Swamy ; Wu, Yi-Kai ; Bille, Josef F. ; Kim, Samuel ; Mao, Xiao Wen ; Gimbel, Howard V. ; Rauser, Michael E. ; Fan, Joseph T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-bfe1e77d8dbc0f69ac716752fd555c2809508f12d7dd19984951f565469e306b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal models</topic><topic>Femtosecond laser</topic><topic>Fluorescein angiography</topic><topic>Indocyanine green angiography</topic><topic>Nonlinear optics</topic><topic>Retinal imaging</topic><topic>Two-photon ophthalmoscope</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jayabalan, Gopal Swamy</creatorcontrib><creatorcontrib>Wu, Yi-Kai</creatorcontrib><creatorcontrib>Bille, Josef F.</creatorcontrib><creatorcontrib>Kim, Samuel</creatorcontrib><creatorcontrib>Mao, Xiao Wen</creatorcontrib><creatorcontrib>Gimbel, Howard V.</creatorcontrib><creatorcontrib>Rauser, Michael E.</creatorcontrib><creatorcontrib>Fan, Joseph T.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jayabalan, Gopal Swamy</au><au>Wu, Yi-Kai</au><au>Bille, Josef F.</au><au>Kim, Samuel</au><au>Mao, Xiao Wen</au><au>Gimbel, Howard V.</au><au>Rauser, Michael E.</au><au>Fan, Joseph T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo two-photon imaging of retina in rabbits and rats</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>166</volume><spage>40</spage><epage>48</epage><pages>40-48</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>The purpose of this study was to evaluate the retina using near-infrared (NIR) two-photon scanning laser ophthalmoscopy. New Zealand white rabbits, albino rats, and brown Norway rats were used in this study. An autofluorescence image of the retina, including the retinal cells and its associated vasculatures was obtained by a real-time scan using the ophthalmoscope. Furthermore, the retinal vessels, nerve fiber layers and the non-pigmented retina were recorded with two-photon fluorescein angiography (FA); and the choroidal vasculatures were recorded using two-photon indocyanine green angiography (ICGA). Two-photon ICGA was achieved by exciting a second singlet state at ∼398 nm. Simultaneous two-photon FA and two-photon ICGA were performed to characterize the retinal and choroidal vessels with a single injection. The minimum laser power threshold required to elicit two-photon fluorescence was determined. The two-photon ophthalmoscope could serve as a promising tool to detect and monitor the disease progression in animal models. Moreover, these high-resolution images of retinal and choroidal vessels can be acquired in a real-time scan with a single light source, requiring no additional filters for FA or ICGA. The combination of FA and ICGA using the two-photon ophthalmoscope will help researchers to characterize the retinal diseases in animal models, and also to classify the types (classic, occult or mixed) of choroidal neovascularization (CNV) in macular degeneration. Furthermore, the prototype can be adapted to image the retina of rodents and rabbits.
•Two-photon excited fluorescence imaging at 780 nm allows multimodal fundus imaging with high quality and contrast.•Two-photon fundus imaging with a 30° field of view is significant.•Demonstrated two-photon fluorescein angiography (FA) and two-photon indocyanine green angiography (ICGA).•Investigated the two-photon excitation and emission of second singlet state of indocyanine green dye.•Simultaneous two-photon FA and ICGA with a single light source were achieved requiring no additional filters.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28483661</pmid><doi>10.1016/j.exer.2017.04.009</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0494-7459</orcidid><orcidid>https://orcid.org/0000-0002-5648-9664</orcidid></addata></record> |
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| subjects | Animal models Femtosecond laser Fluorescein angiography Indocyanine green angiography Nonlinear optics Retinal imaging Two-photon ophthalmoscope |
| title | In vivo two-photon imaging of retina in rabbits and rats |
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