Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity
Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin...
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| Veröffentlicht in: | Parasitology 2017-08, Vol.144 (9), p.1179-1190 |
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| creator | CEOLE, LIGIA FERNANDA CARDOSO, MARIA DAS GRAÇAS SOARES, MAURILIO JOSÉ |
| description | Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL−1. The main constituent (nerolidol: 25·22%) presented a similar inhibitory effect (IC50/24 h = 74·3 µg mL−1). Ultrastructural observation of nerolidol-treated parasites by scanning and transmission electron microscopies revealed cell shrinkage and morphological alterations in the mitochondrion, nuclear chromatin and flagellar pocket. Flow cytometry analysis showed a reduction in the cell size, loss of mitochondrial membrane potential, phosphatidylserine exposure and DNA degradation, which when associated with the morphological changes indicated that nerolidol induced incidental cell death in the L. braziliensis promastigotes. The results presented here indicate that nerolidol derivatives are promising compounds for further evaluation against Leishmania parasites. |
| doi_str_mv | 10.1017/S0031182017000452 |
| format | Article |
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The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL−1. The main constituent (nerolidol: 25·22%) presented a similar inhibitory effect (IC50/24 h = 74·3 µg mL−1). Ultrastructural observation of nerolidol-treated parasites by scanning and transmission electron microscopies revealed cell shrinkage and morphological alterations in the mitochondrion, nuclear chromatin and flagellar pocket. Flow cytometry analysis showed a reduction in the cell size, loss of mitochondrial membrane potential, phosphatidylserine exposure and DNA degradation, which when associated with the morphological changes indicated that nerolidol induced incidental cell death in the L. braziliensis promastigotes. The results presented here indicate that nerolidol derivatives are promising compounds for further evaluation against Leishmania parasites.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182017000452</identifier><identifier>PMID: 28482935</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Apoptosis ; Cell death ; Cell size ; Chromatin ; Cytometry ; Deoxyribonucleic acid ; DNA ; Essential oils ; Flagella ; Flow cytometry ; Herbal medicine ; Humans ; Infectious diseases ; Leishmania ; Leishmania braziliensis ; Leishmania braziliensis - drug effects ; Leishmaniasis, Mucocutaneous - drug therapy ; Leishmaniasis, Mucocutaneous - parasitology ; Membrane potential ; Microbicides ; Mitochondria ; Mucocutaneous leishmaniasis ; Nerolidol ; Oils & fats ; Oils, Volatile - chemistry ; Oils, Volatile - pharmacology ; Parasites ; Parasitic diseases ; Phosphatidylserine ; Piper - chemistry ; Piper aduncum ; Piper longum ; Plant mitochondria ; Plant Oils - chemistry ; Plant Oils - pharmacology ; Promastigotes ; Protozoa ; Sesquiterpenes - chemistry ; Sesquiterpenes - pharmacology ; Shrinkage ; THP-1 Cells ; Vector-borne diseases</subject><ispartof>Parasitology, 2017-08, Vol.144 (9), p.1179-1190</ispartof><rights>Copyright © Cambridge University Press 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-3f91b803ac5eeb2e915c42558ba3fc3a4e55d22a5df2cc5a8bd6e8500292fede3</citedby><cites>FETCH-LOGICAL-c373t-3f91b803ac5eeb2e915c42558ba3fc3a4e55d22a5df2cc5a8bd6e8500292fede3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182017000452/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27903,27904,55606</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28482935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CEOLE, LIGIA FERNANDA</creatorcontrib><creatorcontrib>CARDOSO, MARIA DAS GRAÇAS</creatorcontrib><creatorcontrib>SOARES, MAURILIO JOSÉ</creatorcontrib><title>Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL−1. The main constituent (nerolidol: 25·22%) presented a similar inhibitory effect (IC50/24 h = 74·3 µg mL−1). Ultrastructural observation of nerolidol-treated parasites by scanning and transmission electron microscopies revealed cell shrinkage and morphological alterations in the mitochondrion, nuclear chromatin and flagellar pocket. Flow cytometry analysis showed a reduction in the cell size, loss of mitochondrial membrane potential, phosphatidylserine exposure and DNA degradation, which when associated with the morphological changes indicated that nerolidol induced incidental cell death in the L. braziliensis promastigotes. The results presented here indicate that nerolidol derivatives are promising compounds for further evaluation against Leishmania parasites.</description><subject>Apoptosis</subject><subject>Cell death</subject><subject>Cell size</subject><subject>Chromatin</subject><subject>Cytometry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Essential oils</subject><subject>Flagella</subject><subject>Flow cytometry</subject><subject>Herbal medicine</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Leishmania</subject><subject>Leishmania braziliensis</subject><subject>Leishmania braziliensis - drug effects</subject><subject>Leishmaniasis, Mucocutaneous - drug therapy</subject><subject>Leishmaniasis, Mucocutaneous - parasitology</subject><subject>Membrane potential</subject><subject>Microbicides</subject><subject>Mitochondria</subject><subject>Mucocutaneous leishmaniasis</subject><subject>Nerolidol</subject><subject>Oils & fats</subject><subject>Oils, Volatile - chemistry</subject><subject>Oils, Volatile - pharmacology</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Phosphatidylserine</subject><subject>Piper - chemistry</subject><subject>Piper aduncum</subject><subject>Piper longum</subject><subject>Plant mitochondria</subject><subject>Plant Oils - chemistry</subject><subject>Plant Oils - pharmacology</subject><subject>Promastigotes</subject><subject>Protozoa</subject><subject>Sesquiterpenes - chemistry</subject><subject>Sesquiterpenes - pharmacology</subject><subject>Shrinkage</subject><subject>THP-1 Cells</subject><subject>Vector-borne diseases</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kU9P3DAQxS1E1V22_QBckCUuHBrwnzg4R4SgVFoBEu05mjiT7qySeLETJPj09YptVYE4eez3e8-jGcYOpTiVQp6fPQihpbQq1UKI3Kg9Npd5UWZWFnKfzbdyttVn7CDGdWIKXajPbKZsblWpzZzRLQbfUeO7b3xcIe-BBu78EEcaJxxG7lt-TxsMHJppcFPPMcb0TtBxT8m0gsgh3bMlUlz1MBDwOsALdYRDpCS6kZ5ofP7CPrXQRfy6Oxfs1_XVz8ubbHn3_cflxTJz-lyPmW5LWVuhwRnEWmEpjcuVMbYG3ToNORrTKAWmaZVzBmzdFGiNEKpULTaoF-zkNXcT_OOEcax6ig67Dgb0U6ykLQtb6kLLhB6_Qdd-CkPqrpKlya3IdZrYgslXygUfY8C22gTqITxXUlTbPVTv9pA8R7vkqe6x-ef4O_gE6F0o9HWg5jf-9_eHsX8ATpOSyg</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>CEOLE, LIGIA FERNANDA</creator><creator>CARDOSO, MARIA DAS GRAÇAS</creator><creator>SOARES, MAURILIO JOSÉ</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity</title><author>CEOLE, LIGIA FERNANDA ; CARDOSO, MARIA DAS GRAÇAS ; SOARES, MAURILIO JOSÉ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-3f91b803ac5eeb2e915c42558ba3fc3a4e55d22a5df2cc5a8bd6e8500292fede3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Apoptosis</topic><topic>Cell death</topic><topic>Cell size</topic><topic>Chromatin</topic><topic>Cytometry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Essential oils</topic><topic>Flagella</topic><topic>Flow cytometry</topic><topic>Herbal medicine</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Leishmania</topic><topic>Leishmania braziliensis</topic><topic>Leishmania braziliensis - 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Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CEOLE, LIGIA FERNANDA</au><au>CARDOSO, MARIA DAS GRAÇAS</au><au>SOARES, MAURILIO JOSÉ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>144</volume><issue>9</issue><spage>1179</spage><epage>1190</epage><pages>1179-1190</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><abstract>Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL−1. The main constituent (nerolidol: 25·22%) presented a similar inhibitory effect (IC50/24 h = 74·3 µg mL−1). Ultrastructural observation of nerolidol-treated parasites by scanning and transmission electron microscopies revealed cell shrinkage and morphological alterations in the mitochondrion, nuclear chromatin and flagellar pocket. Flow cytometry analysis showed a reduction in the cell size, loss of mitochondrial membrane potential, phosphatidylserine exposure and DNA degradation, which when associated with the morphological changes indicated that nerolidol induced incidental cell death in the L. braziliensis promastigotes. The results presented here indicate that nerolidol derivatives are promising compounds for further evaluation against Leishmania parasites.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>28482935</pmid><doi>10.1017/S0031182017000452</doi><tpages>12</tpages></addata></record> |
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| subjects | Apoptosis Cell death Cell size Chromatin Cytometry Deoxyribonucleic acid DNA Essential oils Flagella Flow cytometry Herbal medicine Humans Infectious diseases Leishmania Leishmania braziliensis Leishmania braziliensis - drug effects Leishmaniasis, Mucocutaneous - drug therapy Leishmaniasis, Mucocutaneous - parasitology Membrane potential Microbicides Mitochondria Mucocutaneous leishmaniasis Nerolidol Oils & fats Oils, Volatile - chemistry Oils, Volatile - pharmacology Parasites Parasitic diseases Phosphatidylserine Piper - chemistry Piper aduncum Piper longum Plant mitochondria Plant Oils - chemistry Plant Oils - pharmacology Promastigotes Protozoa Sesquiterpenes - chemistry Sesquiterpenes - pharmacology Shrinkage THP-1 Cells Vector-borne diseases |
| title | Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity |
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