Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model
Free radicals and neutrophils are potent sources of ischemia-reperfusion injury (I/R) and they can be limited by the use of exogenous application of some therapeutic agents. The objective of this study was to compare the effects of cilostazol and diltiazem hydrochloride in a rat hind limb model of I...
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| Veröffentlicht in: | The Heart surgery forum 2017-04, Vol.20 (2), p.E058-E065 |
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| creator | Inan, Bekir Sönmez Ergün, Selma Nurten, Asiye Küçükgergin, Canan Zengin Türkmen, Aslı Seçkin, Şule Erkalp, Kerem Ziyade, Sedat |
| description | Free radicals and neutrophils are potent sources of ischemia-reperfusion injury (I/R) and they can be limited by the use of exogenous application of some therapeutic agents. The objective of this study was to compare the effects of cilostazol and diltiazem hydrochloride in a rat hind limb model of I/R injury. Methods: Skeletal muscles submitted to 2 hours of ischemia by placing an aneurysm clip to femoral artery and reperfused after 1, 2 and 4 hours. Seventy-two Wistar-Albino rats were randomly divided into mainly four groups according to treatment agents: Group I (control group) was treated with saline; Group II was treated with diltiazem hydrochloride; Group III was treated with cilostazol in 30% dimethyl sulphoxide; and Group IV was treated with 30% dimethyl sulphoxide intraperitoneally. These four main groups also subdivided into three subgroups according to duration of the reperfusion times. Blood samples were taken and all rats were sacrificed. Results: Cilostazol-treated groups demonstrated a significant decrease in tissue and serum malondialdehyde (MDA) levels, and tissue myeloperoxidase (MPO ) activity compared with other groups. Increase in serum nitric oxide (NOx) level was significantly higher in all subgroups of cilastazol, diltiazem hydrochloride, and dimethyl sulphoxide groups versus the control group.
Although these results suggest the beneficial effects of cilostazol and diltiazem hydrochloride on I/R injury, the effect of cilostazol on I/R injury seems to be more efficient than diltiazem hydrochloride. |
| doi_str_mv | 10.1532/hsf.1663 |
| format | Article |
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Although these results suggest the beneficial effects of cilostazol and diltiazem hydrochloride on I/R injury, the effect of cilostazol on I/R injury seems to be more efficient than diltiazem hydrochloride.</description><identifier>ISSN: 1098-3511</identifier><identifier>EISSN: 1522-6662</identifier><identifier>DOI: 10.1532/hsf.1663</identifier><identifier>PMID: 28481745</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Calcium Channel Blockers - therapeutic use ; Diltiazem - therapeutic use ; Disease Models, Animal ; Drug Therapy, Combination ; Hindlimb - blood supply ; Male ; Rats ; Rats, Wistar ; Reperfusion Injury - drug therapy ; Tetrazoles - therapeutic use ; Treatment Outcome ; Vasodilator Agents - therapeutic use</subject><ispartof>The Heart surgery forum, 2017-04, Vol.20 (2), p.E058-E065</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c213t-bcd1346c6f54f91a89c7d713c689c5ff248eb3b2c67fda22a77e074969bbb48d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28481745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inan, Bekir</creatorcontrib><creatorcontrib>Sönmez Ergün, Selma</creatorcontrib><creatorcontrib>Nurten, Asiye</creatorcontrib><creatorcontrib>Küçükgergin, Canan</creatorcontrib><creatorcontrib>Zengin Türkmen, Aslı</creatorcontrib><creatorcontrib>Seçkin, Şule</creatorcontrib><creatorcontrib>Erkalp, Kerem</creatorcontrib><creatorcontrib>Ziyade, Sedat</creatorcontrib><title>Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model</title><title>The Heart surgery forum</title><addtitle>Heart Surg Forum</addtitle><description>Free radicals and neutrophils are potent sources of ischemia-reperfusion injury (I/R) and they can be limited by the use of exogenous application of some therapeutic agents. The objective of this study was to compare the effects of cilostazol and diltiazem hydrochloride in a rat hind limb model of I/R injury. Methods: Skeletal muscles submitted to 2 hours of ischemia by placing an aneurysm clip to femoral artery and reperfused after 1, 2 and 4 hours. Seventy-two Wistar-Albino rats were randomly divided into mainly four groups according to treatment agents: Group I (control group) was treated with saline; Group II was treated with diltiazem hydrochloride; Group III was treated with cilostazol in 30% dimethyl sulphoxide; and Group IV was treated with 30% dimethyl sulphoxide intraperitoneally. These four main groups also subdivided into three subgroups according to duration of the reperfusion times. Blood samples were taken and all rats were sacrificed. Results: Cilostazol-treated groups demonstrated a significant decrease in tissue and serum malondialdehyde (MDA) levels, and tissue myeloperoxidase (MPO ) activity compared with other groups. Increase in serum nitric oxide (NOx) level was significantly higher in all subgroups of cilastazol, diltiazem hydrochloride, and dimethyl sulphoxide groups versus the control group.
Although these results suggest the beneficial effects of cilostazol and diltiazem hydrochloride on I/R injury, the effect of cilostazol on I/R injury seems to be more efficient than diltiazem hydrochloride.</description><subject>Animals</subject><subject>Calcium Channel Blockers - therapeutic use</subject><subject>Diltiazem - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Drug Therapy, Combination</subject><subject>Hindlimb - blood supply</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reperfusion Injury - drug therapy</subject><subject>Tetrazoles - therapeutic use</subject><subject>Treatment Outcome</subject><subject>Vasodilator Agents - therapeutic use</subject><issn>1098-3511</issn><issn>1522-6662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAAhYMoTqfgXyA5eulskjZJjzKnG0yEoeeSnzQjbWbSHra_3g6nnt7j8fEOHwB3KJ-hkuDHJtkZopScgStUYpxRSvH52POKZ6REaAKuU9rmOaYY00swwbzgiBXlFWgW1hrVJxgsnDsfUi8OwUPRafjsfO_EwbRwudcxqMaH6LSBoYOrpBrTOpFtzM5EOyR3HLvtEPfQdVDAjejh0nXau1bCt6CNvwEXVvhkbk85BZ8vi4_5Mlu_v67mT-tMYUT6TCqNSEEVtWVhKyR4pZhmiCg6ttJaXHAjicSKMqsFxoIxk7OiopWUsuCaTMHDz-8uhq_BpL5uXVLGe9GZMKQa8YpyzktW_aMqhpSisfUuulbEfY3y-ui1Hr3WR68jen96HWRr9B_4K5J8A1t9c_s</recordid><startdate>20170429</startdate><enddate>20170429</enddate><creator>Inan, Bekir</creator><creator>Sönmez Ergün, Selma</creator><creator>Nurten, Asiye</creator><creator>Küçükgergin, Canan</creator><creator>Zengin Türkmen, Aslı</creator><creator>Seçkin, Şule</creator><creator>Erkalp, Kerem</creator><creator>Ziyade, Sedat</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170429</creationdate><title>Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model</title><author>Inan, Bekir ; Sönmez Ergün, Selma ; Nurten, Asiye ; Küçükgergin, Canan ; Zengin Türkmen, Aslı ; Seçkin, Şule ; Erkalp, Kerem ; Ziyade, Sedat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c213t-bcd1346c6f54f91a89c7d713c689c5ff248eb3b2c67fda22a77e074969bbb48d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Calcium Channel Blockers - therapeutic use</topic><topic>Diltiazem - therapeutic use</topic><topic>Disease Models, Animal</topic><topic>Drug Therapy, Combination</topic><topic>Hindlimb - blood supply</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reperfusion Injury - drug therapy</topic><topic>Tetrazoles - therapeutic use</topic><topic>Treatment Outcome</topic><topic>Vasodilator Agents - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inan, Bekir</creatorcontrib><creatorcontrib>Sönmez Ergün, Selma</creatorcontrib><creatorcontrib>Nurten, Asiye</creatorcontrib><creatorcontrib>Küçükgergin, Canan</creatorcontrib><creatorcontrib>Zengin Türkmen, Aslı</creatorcontrib><creatorcontrib>Seçkin, Şule</creatorcontrib><creatorcontrib>Erkalp, Kerem</creatorcontrib><creatorcontrib>Ziyade, Sedat</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Heart surgery forum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inan, Bekir</au><au>Sönmez Ergün, Selma</au><au>Nurten, Asiye</au><au>Küçükgergin, Canan</au><au>Zengin Türkmen, Aslı</au><au>Seçkin, Şule</au><au>Erkalp, Kerem</au><au>Ziyade, Sedat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model</atitle><jtitle>The Heart surgery forum</jtitle><addtitle>Heart Surg Forum</addtitle><date>2017-04-29</date><risdate>2017</risdate><volume>20</volume><issue>2</issue><spage>E058</spage><epage>E065</epage><pages>E058-E065</pages><issn>1098-3511</issn><eissn>1522-6662</eissn><abstract>Free radicals and neutrophils are potent sources of ischemia-reperfusion injury (I/R) and they can be limited by the use of exogenous application of some therapeutic agents. The objective of this study was to compare the effects of cilostazol and diltiazem hydrochloride in a rat hind limb model of I/R injury. Methods: Skeletal muscles submitted to 2 hours of ischemia by placing an aneurysm clip to femoral artery and reperfused after 1, 2 and 4 hours. Seventy-two Wistar-Albino rats were randomly divided into mainly four groups according to treatment agents: Group I (control group) was treated with saline; Group II was treated with diltiazem hydrochloride; Group III was treated with cilostazol in 30% dimethyl sulphoxide; and Group IV was treated with 30% dimethyl sulphoxide intraperitoneally. These four main groups also subdivided into three subgroups according to duration of the reperfusion times. Blood samples were taken and all rats were sacrificed. Results: Cilostazol-treated groups demonstrated a significant decrease in tissue and serum malondialdehyde (MDA) levels, and tissue myeloperoxidase (MPO ) activity compared with other groups. Increase in serum nitric oxide (NOx) level was significantly higher in all subgroups of cilastazol, diltiazem hydrochloride, and dimethyl sulphoxide groups versus the control group.
Although these results suggest the beneficial effects of cilostazol and diltiazem hydrochloride on I/R injury, the effect of cilostazol on I/R injury seems to be more efficient than diltiazem hydrochloride.</abstract><cop>United States</cop><pmid>28481745</pmid><doi>10.1532/hsf.1663</doi></addata></record> |
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| subjects | Animals Calcium Channel Blockers - therapeutic use Diltiazem - therapeutic use Disease Models, Animal Drug Therapy, Combination Hindlimb - blood supply Male Rats Rats, Wistar Reperfusion Injury - drug therapy Tetrazoles - therapeutic use Treatment Outcome Vasodilator Agents - therapeutic use |
| title | Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model |
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